Asunto(s)
Antígenos/inmunología , Concanavalina A/farmacología , Liberación de Histamina , Leucocitos/inmunología , Absceso Hepático Amebiano/inmunología , Adolescente , Adulto , Anciano , Entamoeba histolytica/inmunología , Femenino , Humanos , Absceso Hepático Amebiano/patología , Masculino , Persona de Mediana EdadRESUMEN
Se estudio la liberacion de histamina leucocitaria en pacientes con absceso hepatico amibiano agudo, absceso curado, condiciones agudas no amibianas y testigos sanos, utilizando un antigeno amibiano (histoliticina) y un liberador inespecifico (concanavalina A).La liberacion de histamina en leucocitos de pacientes con absceso hepatico amibiano agudo requirio de cantidades significativamente menores de histoliticina que en los leucocitos de los otros tres grupos estudiados, empero no fue diferente utilizando concanavalina A.Se sugiere un estado de hipersensibilidad inmediata a la histoliticina com probable explicacion de este fenomeno en los pacientes con absceso hepatico amibiano agudo. La histamina asi liberada podria contribuir a modular la inflamacion temprana y tardia en la amibiasis invasora
Asunto(s)
Adolescente , Adulto , Persona de Mediana Edad , Humanos , Masculino , Femenino , Liberación de Histamina , Técnicas In Vitro , Absceso Hepático Amebiano , Concanavalina A , LeucocitosAsunto(s)
Implantación del Embrión , Fertilización , Cigoto , Animales , Femenino , Humanos , Mamíferos/fisiologíaRESUMEN
A transmission electron microscopy study was carried out in prospective implantation sites in uterine horns of rats of the Long Evans strain. The purpose was to search for possible modifications in glycoproteins of outer coats of endometrial epithelium shortly before blastocyst-endometrial contact Shea's technique (Lanthanum-Alcian blue) was used to visualize glycoproteins. Prospective implantation sites disclosed a rather thin finely granular electrondense layer along the plasma membrane of the surface endometrial cells. In addition, there was a decrease in the number of microvilli as well as a lack of Lanthanum precipitate in junctional complexes. Sites remote from prospective implantation areas, disclosed a thicker electrondense layer of glycoproteins in cell plasma membranes including those of well developed microvilli. The junctional complexes were well depicted by the electrodense lanthanum salt precipitates. These observations suggest that a mechanism other than a more physical contact take place and induce the above changes.
Asunto(s)
Blastómeros/análisis , Endometrio/citología , Glicoproteínas/análisis , Animales , Blastómeros/ultraestructura , Implantación del Embrión , Endometrio/análisis , Femenino , Membrana Mucosa/citología , RatasRESUMEN
The fixed position of trophoblast in the uterus is preceded by zygote orientation and its contact, adhession and fusion with endometrial cell membrane. These interactions take place in a precise site called "implantation site". The identification of this area by the administration of pontamine blue has allowed biochemical studies which showed an increase (in the site) of glycolytic enzymes activity of the pentose cycle, of beta-glucuramidase and of acid and alkaline phosphatase; with a decrease in catepsine D activity. In non implanted tissue, isocitric dehydrogenase activity is decreased. From the biochemical standpoint the implanted endometrium may be different from the rest, which allows a better knowledge of possible molecular mechanisms related with implantation, as well as the possible inhibition with medication.