RESUMEN

Tubulin glutamylation is a post-translational modification that occurs predominantly in the ciliary axoneme and has been suggested to be important for ciliary function. However, its relationship to disorders of the primary cilium, termed ciliopathies, has not been explored. Here we mapped a new locus for Joubert syndrome (JBTS), which we have designated as JBTS15, and identified causative mutations in CEP41, which encodes a 41-kDa centrosomal protein. We show that CEP41 is localized to the basal body and primary cilia, and regulates ciliary entry of TTLL6, an evolutionarily conserved polyglutamylase enzyme. Depletion of CEP41 causes ciliopathy-related phenotypes in zebrafish and mice and results in glutamylation defects in the ciliary axoneme. Our data identify CEP41 mutations as a cause of JBTS and implicate tubulin post-translational modification in the pathogenesis of human ciliary dysfunction.

Asunto(s)

Enfermedades Cerebelosas/genética , Cilios/genética , Trastornos de la Motilidad Ciliar/genética , Anomalías del Ojo/genética , Ácido Glutámico/metabolismo , Enfermedades Renales Poliquísticas/genética , Proteínas/genética , Tubulina (Proteína)/metabolismo , Animales , Centrosoma/metabolismo , Mapeo Cromosómico , Cilios/metabolismo , Femenino , Sitios Genéticos , Humanos , Masculino , Ratones , Mutación , Péptido Sintasas/metabolismo , Polimorfismo de Nucleótido Simple , Procesamiento Proteico-Postraduccional , Síndrome