RESUMEN
The influence of multipotent mesenchymal stromal cells (MMSC), got from allogenic bone marrow, on local radiation injury in rats after beta-irradiation by a source 90Sr/90Y in a dose 140 Gy under various conditions and ways transplantation was studied. It was established, that transplantation MMSC, allocated on biodegradative membranes, which have been carried out for 21 day after an irradiation in conditions minor surgical of ulcers, resulted in reduction of the area of local beam defeats and acceleration of healing of skin, in comparison with the control of an irradiation. The introduction of suspension MMSC subcutaneous around of the center of a defeat at 8 day after an irradiation caused earlier healing of ulcers. It was concluded, that application of MMSC is perspective for treatment of local radiation injury and necessity of development of optimum conditions of their use at cell therapy of radiation injury of skin.
Asunto(s)
Traumatismos de la Espalda/cirugía , Trasplante de Células Madre Mesenquimatosas/métodos , Células Madre Mesenquimatosas/fisiología , Células Madre Multipotentes/fisiología , Traumatismos Experimentales por Radiación/cirugía , Úlcera Cutánea/cirugía , Animales , Traumatismos de la Espalda/etiología , Partículas beta , Células Inmovilizadas/trasplante , Masculino , Células Madre Multipotentes/trasplante , Ratas , Ratas Wistar , Úlcera Cutánea/etiología , Cicatrización de HeridasRESUMEN
We compared the effects of transplantation of fetal fibroblasts and fibroblast-like mesenchymal stem cells of the bone marrow on healing of deep burn wound in rats. It was found that transplantation of fetal fibroblasts and fibroblast-like mesenchymal stem cells on the burn surface reduces cell infiltration, promotes the formation of vessels and granulation tissue, which creates conditions for more rapid healing of the burn wounds.
Asunto(s)
Quemaduras/patología , Quemaduras/terapia , Inflamación/prevención & control , Trasplante de Células Madre Mesenquimatosas , Cicatrización de Heridas/fisiología , Animales , Biopsia , Fibroblastos/trasplante , Técnicas Histológicas , Masculino , Ratas , Ratas WistarRESUMEN
A combined graft consisting of a free full-thickness skin flap and cultured autologous fibroblast-like bone marrow mesenchymal stem cells was effectively implanted and healed on the facial soft tissue defect after removal of a pathological vascular conglomeration in a female patient with congenital arteriovenous macrofistulous dysplasia. In order to reduce bloodflow intensity and arteriovenous shunting, repeated endovascular occlusion and transcutaneous ligature of regional vessels from the carotid artery basin feeding the pathological zone was carried out followed by resection of this tumor-like vascular formation.
Asunto(s)
Fístula Arteriovenosa/congénito , Fístula Arteriovenosa/terapia , Trasplante de Médula Ósea/métodos , Trasplante de Células Madre Mesenquimatosas/métodos , Trasplante de Piel/métodos , Cicatrización de Heridas/fisiología , Adulto , Fístula Arteriovenosa/patología , Fístula Arteriovenosa/cirugía , Vasos Sanguíneos/diagnóstico por imagen , Cara/irrigación sanguínea , Cara/patología , Femenino , Humanos , Ligadura/métodos , Microscopía Fluorescente , Resultado del Tratamiento , UltrasonografíaRESUMEN
Original recombinant adenoviral construction carrying E. coli beta-galactosidase LacZ gene designed by the authors is convenient for labeling and monitoring of bone marrow mesenchymal (stromal) progenitor cells and myocardial and skin fetal cells transplanted in damaged rat tissues (in the perinecrotic zone of the myocardium and onto burnt skin surface) for their reparation. This genetic construction after pre-inactivation of endogenous beta-galactosidase allows to detect transplanted cells in the foci of injury; positive effects of transplantation on tissue reparation processes can be attributed to the presence of transplanted cells.
Asunto(s)
Técnicas de Transferencia de Gen , Vectores Genéticos/genética , Trasplante de Células Madre Mesenquimatosas/métodos , Células Madre Mesenquimatosas/citología , Adenoviridae , Animales , Escherichia coli , Técnicas Histológicas , Operón Lac/genética , Ratas , beta-Galactosidasa/genéticaRESUMEN
The effects of autologous bone marrow stromal cells immobilized on ElastoPHB membranes on reparative processes were studied on a model of rat skeletal muscle injury. Bone marrow stromal cells inhibited substitute (sclerosing) regeneration and activated reparative (reconstructive) regeneration of tissues.
Asunto(s)
Células de la Médula Ósea/citología , Trasplante de Médula Ósea/métodos , Membranas Artificiales , Músculo Esquelético/fisiología , Regeneración/fisiología , Adenoviridae , Animales , Galactósidos , Vectores Genéticos/genética , Inmunohistoquímica , Indoles , Operón Lac/genética , Músculo Esquelético/lesiones , Ratas , Ratas Wistar , Células del Estroma/trasplante , beta-Galactosidasa/metabolismoRESUMEN
Female patient with extensive skin burn (I-II-IIIAB skin burn, total area 40%, area of IIIB degree 30%) was treated using transplantation of allogenic fibroblast-like bone marrow mesenchymal stem cells onto the surface of deep thermal burn. The study of wound healing dynamics after transplantation of allogenic fibroblast-like mesenchymal stem cells confirmed high tempo of wound regeneration in the presence of active neoangiogenesis. Due to this, autodermoplasty of burn wounds could be carried out with good results as early as on day 4 after transplantation of fibroblast-like mesenchymal stem cells; this led to more rapid healing of donor zones and accelerated rehabilitation of the patient.
Asunto(s)
Células de la Médula Ósea/citología , Quemaduras/terapia , Fibroblastos/trasplante , Trasplante de Células Madre Mesenquimatosas , Piel/lesiones , Quemaduras/patología , Femenino , Humanos , Persona de Mediana Edad , Piel/patología , Cicatrización de Heridas/fisiologíaRESUMEN
Transplantation of suspention of allogenic fibroblast-like mesenchymal stem cells (AFMSC) of the bone marrow and AFMSC immobilized on the biodegradable membrane (BM) stimulates healing of deep burn wounds compared to control (without cell transplantation) because these cells produce biologically active substances into the wound. AFMSC immobilized on BM activate repair processes in the wounds earlier. This may be due to their higher functional activity created in the monolayer by adequate intercellular interactions. To avoid complications caused by low diffuse properties of BM (protein precipitation), BM should be removed from the surface of the burn wound maximum 3 days after its application.
Asunto(s)
Materiales Biocompatibles/metabolismo , Quemaduras/terapia , Células Inmovilizadas , Células Madre Mesenquimatosas , Trasplante de Células Madre/métodos , Animales , Células de la Médula Ósea/citología , Quemaduras/patología , Técnicas de Cultivo de Célula/instrumentación , Técnicas de Cultivo de Célula/métodos , Fibroblastos/citología , Masculino , Células Madre Mesenquimatosas/citología , Ratas , Ratas WistarRESUMEN
Regeneration of deep burn wounds after transplantation of allogenic and autogenic fibroblast-like bone marrow mesenchymal stem cells and embryonic fibroblasts on burn surface was studied in 40 Wistar rats. Transplantation of allogenic and autogenic fibroblast-like bone marrow mesenchymal stem cells and transplantation of embryonic fibroblasts decreased cell infiltration of the wound and accelerated the formation of new vessels and granulation tissue in the wound in comparison with the control (burn wounds without cell transplantation). Regeneration processes were most active after transplantation of fibroblast-like bone marrow mesenchymal stem cells, in particular, autogenic cells, which was confirmed by more rapid decrease in burn surface area. Wound healing after transplantation of fibroblast-like bone marrow mesenchymal cells and embryonic fibroblasts was associated with long functioning of transplanted cells (as was shown by staining for beta-galactosidase, the cells were transfected with an adenovirus vector carrying the marker gene). It is hypothesized that more rapid regeneration of burn wounds after transplantation of fibroblast-like bone marrow mesenchymal stem cells was due to low differentiation of these cells in comparison with embryonic fibroblasts.
Asunto(s)
Células de la Médula Ósea/metabolismo , Quemaduras , Fibroblastos/metabolismo , Células Madre Hematopoyéticas/fisiología , Regeneración/fisiología , Cicatrización de Heridas , Animales , Biopsia , Embrión de Mamíferos/anatomía & histología , Femenino , Trasplante de Células Madre Hematopoyéticas , Masculino , Mesodermo/citología , Embarazo , Ratas , Ratas Wistar , Piel/metabolismo , Piel/patologíaRESUMEN
The opportunity of faster closing deep burn wounds by using fibroblast-like mesenchymal bone marrow stem cells (FMSC) and embryonic fibroblasts (EF) was investigated in rats. It was shown that FMSC as well as EF transplanted onto burn surfaces reduced the expression of cell infiltration but accelerated the formation of vessels de novo and granulation tissue in the wounds. These changes form the conditions for faster closing the burn wounds as compared to the control wounds (without cell transplantation). High rate of wound closing induced by FMSC and EF is thought to be caused by a long period (up to 30 days) of vital activity of the cells grafted on the burn surface. It was also found out that the rate of wound regeneration induced by FMSC was higher that that induced by EF.