RESUMEN
Lowering extracellular calcium in cultures of human diploid fibroblast-like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca2+ and was inhibited by 3-aminobenzamide, an inhibitor of ADP-ribosyl transfer reactions. The concentrations of 3-aminobenzamide needed to inhibit the loss of NAD were consistent with those required to inhibit cellular mono(ADP-ribosyl) rather than poly(ADP-ribosyl) reactions. Calcium depletion did not inhibit the biosynthesis of NAD. These results suggest that mono(ADP-ribosyl)ation is involved in the regulation of cellular Ca2+ levels.
Asunto(s)
Adenosina Difosfato Ribosa/metabolismo , Calcio/metabolismo , Espacio Extracelular/metabolismo , Piel/metabolismo , Células Cultivadas , Fibroblastos/metabolismo , Homeostasis , Humanos , Masculino , NAD/metabolismo , Piel/citología , Estimulación QuímicaRESUMEN
The Scale of Knowledge and Attitudes Toward Epilepsy and Persons with Epilepsy (ATPE) was developed as a contemporary, brief, easy to administer and score, psychometrically sound instrument. The 32 item scale consists of 25 statements from which an attitude score is derived and 13 statements from which a knowledge score is derived. Data were collected and analyzed which indicate satisfactory item characteristics, scale reliability and internal consistency. Scale development supports the content validity of the scale, while data from multiple regression and factor analyses support its construct validity. The scale should be useful for the investigation of questions concerning the formation, structure, correlates and modification of knowledge and attitudes regarding epilepsy and persons with epilepsy.