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OBJECTIVES: The knowledge and awareness surrounding dental implants have significantly increased over the years, driven by advancements in technology, improved educational resources, and increased accessibility to dental care. Despite their widespread use, it is essential to assess the level of knowledge and awareness among patients regarding dental implants. The purpose of the present study is to measure the knowledge, awareness and decision making in dental implant therapy in North Indian population. SETTINGS AND DESIGN: An analytical closed ended questionnaire based study. METHODS: A cross-sectional well structured questionnaire survey was conducted amongst 300 out patients randomly either by interview or Google form to assess the knowledge and awareness about dental implant as a treatment modality. There were seven questions which were close ended multiple choice type concerning patient knowledge and 10 questions for assessing awareness and decision making towards dental implants. Date was collected and descriptive analysis was done. RESULTS: 43.67 % of population was either well informed or moderately informed about replacement of missing teeth. For replacement of missing tooth, most of them (83 %) had the knowledge of dental implant therapy. Dentist was the main source of information about implants. 90.67 % studied population believed that implants provide better treatment because of increased chewing efficiency of implants as compared to removable partial dentures or fixed partial dentures. 86.67 % patients thought that implantologist is better qualified that general dental surgeon. 94 % patients were keen to know more about implants. There is non-significant difference in knowledge and awareness as well as decision making for males and females (p<0.05). CONCLUSION: People have partial knowledge about dental implants and there is need of spreading awareness about implant therapy in the region. Dentist plays a crucial role for dissemination of knowledge. Cost is a constraining factor for this implant therapy to be chosen as a treatment modality.

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Background: Stresses on prosthetic crown directly influences the survival rate of implants hence it should be considered while selecting prosthetic material.The aim of the study is to evaluate stress analysis on implant, abutment and peripheral bone with change in different abutment and different crown materials by 3D finite element study. Materials and Methods: A numerical procedure based on finite element method was adopted to investigate the influence of different prosthetic materials and abutment materials on stress situation. Eight different three-dimensional (3D) models of a bone-level implant system and an abutment were created by using the standard tessellation language (STL) data of original implant components. Combinations included of abutment materials i.e., Titanium (Ti), Polyetheretherketone (PEEK), Polyetherketoneketone (PEKK), Polymer infilterated hybrid ceramic (TZI) along with different restoration materials Monolithic Zirconia (MZ) and lithium disilicate glass ceramic (IPS e-max). In each model, the implants were loaded obliquely (150 N). The stress distribution in the implant, Abutment and peripheral bone was evaluated through the von Mises stress analysis. Results: Higher stresses were found on neck of implants irrespective of abutment material and restorative material. Highest stress was found with PEEK material. The pattern of stress distribution in implant and peripheral bone was similar in all models. Conclusion: There is no difference in stresses with the change in restorative material but the change in abutment material has effect on stresses on implants.

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Background: Occlusal loading of osseointegrated implants is believed to be an essential determining factor in the long-term success of an implant treatment. Numerous studies have been conducted on the evaluation of stress distribution by definitive restoration materials for Implant-supported fixed prosthesis, but very few have evaluated provisional restoration materials for the same. This study aims to evaluate the influence of provisional restoration material - Milled Polymethylmethacrylate (PMMA) and Milled Polyetheretherketone (PEEK), over stress distribution on the peri-implant bone around an implant-supported three-unit, fixed dental prosthesis using finite element analysis method. Materials and Methods: Three-dimensional models of a pair of bone-level implant system and titanium base abutments were created using the standard tessellation language data of original implant components. A bone block representing the mandibular posterior area was created, and the implants were placed in the bone block with 100% osseointegration in the 2nd premolar to 2nd molar region. A superstructure of an implant-supported 3-unit bridge was modeled on top of the abutments, each crown to be 8 mm in height and with an outer diameter of 6 mm in 2nd premolar region and 10 mm in 1st molar and 2nd molar region. Two different models were created according to combinations of provisional restoration materials, namely, Milled PMMA and Milled PEEK based on. In each model, the implants were loaded vertically (300 N) and obliquely (150 N at 30°). The stress distribution in the cortical bone, cancellous bone, and implant was evaluated through the von Mises stress analysis. Results: The results showed no difference in stress distribution due to the different provisional restorations - Milled PMMA and Milled PEEK. In addition, the vertical load resulted in higher stress values in the implant components, cortical bone, and cancellous bone in both PEEK and PMMA models as compared to oblique loading. Conclusion: The new polymer, PEEK was seen to provide comparable stress generation in the current study without exceeding the physiological limits of peri-implant bone. Thus, it can be considered as a good alternative to PMMA resin as a provisional crown material since it provides certain additional benefits.

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Bovine mastitis has become a significant economic importance for the dairy industry. Concerns regarding poor milk quality and emergence of bacterial resistance have necessitated to develop an alternative therapeutic approach to antibiotics for the treatment of mastitis. Saturated medium-chain fatty acids (MCFAs) and essential oils (EOs) are known natural antimicrobials, but their combined effect has not been investigated extensively. The objective of the present investigation was to examine the bactericidal effect of various combined treatments of eight EOs and three saturated MCFAs to inactivate predominant mastitis pathogens, including Staphylococcus aureus ATCC 29213; Escherichia coli ATCC 25922; Klebsiella pneumoniae ATCC 27736 and Streptococcus agalactiae ATCC 27956. The minimum inhibitory concentration (MIC) values confirmed that all the tested pathogens were variably susceptible to both EOs and saturated MCFAs. Among essential oils, carvacrol (CAR), trans-cinnamaldehyde (TC) and thymol (TM) showed the highest inhibitory activity at concentration 0·38-1·32 mg/mL. Carvacrol exhibited effective additive antibacterial activity in combined treatment with octanoic acid (OA) in terms of its fractional inhibitory index (0·63-0·88) and time-kill effect in reducing about 6 log CFU/mL bacterial cells in less than 5 min. The effort was also made to elucidate the mechanism of antibacterial action of CAR and OA against selected mastitis pathogens by observing changes in cell microstructure, permeability and integrity of cell membrane and their membrane potential. After adding CAR and OA at MIC level, there were obvious changes in cell morphology, leakage of small electrolytes and macromolecules at the initial few hours of treatment i.e. within 1-2 h were observed. Our results indicated that CAR and OA could be evaluated as alternatives or adjuncts to antibiotics as intramammary infusion or topical application to treat bovine mastitis, significantly improving the microbiological safety of milk.

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In the present investigation, we determined the in vitro antimicrobial activity of eight essential oils (EOs) and three medium-chain fatty acids (MCFAs) alone and in combination against Staphylococcus aureus ATCC 700698, Klebsiella pneumoniae ATCC 700603, and E. coli FcW5. The interactions between EOs and MCFAs were determined by fractional inhibitory concentration indices. Moreover, mode of action of selected bioactive components was studied by changes in bacterial surface charge, morphology, and membrane integrity assays. Among EOs, carvacrol (CAR), trans-cinnamaldehyde (TC), and thymol (TM) showed strong antimicrobial activity. In combination study, CAR+OA (octanoic acid), CAR+DA (decanoic acid), and TM+OA were observed as the most significant (P≤0.05) which were also confirmed through time-kill plots. Based on these results, CAR+OA were found to be most efficacious in terms of killing time (P≤0.05). Changes in the surface charge, morphology, and membrane integrity upon the combined treatment of CAR+OA were also observed, which ultimately leads to cell death. Results suggest that CAR+OA when used in combination offer a significant (P≤0.05) additive antimicrobial activity against the selected pathogenic bacteria. Therefore, these natural bioactive molecules could be interesting alternatives to conventional therapy for the control of mastitis caused by multi-drug-resistant pathogens in bovine animals to ensure the milk safety.

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AIM: This study aims to evaluate and compare the crestal bone levels around implants as influenced by conventional and diode laser during second-stage surgery in an edentulous mandible using cone beam computed tomography (CBCT) and digital radiography (DR). SETTINGS AND DESIGN: A split-mouth in vivo prospective study on edentulous patients involving placement of two implants in mandible followed by the comparison of two different techniques for second-stage surgery. MATERIALS AND METHODS: The study was undertaken to evaluate the crestal bone change around 16 implant sites distributed in two groups (eight implants each) following two methods during second-stage surgery, i.e., Group 1 - Conventional second stage and Group 2 - Diode laser. Measurements were made on two sides (mesial and distal) using intraoral periapical and four sides (labial, lingual, mesial, and distal) using CBCT scans for both groups. These measurements were conducted at two time intervals for both, i.e., immediately after implant loading and twenty 4 weeks after implant loading. The values obtained were subjected to statistical analysis. STATISTICAL ANALYSIS USED: The normality of data was checked by Shapiro-Wilk's test. Intragroup comparison was compared using independent t-test by post hoc comparison by Bonferroni method (P < 0.05). RESULTS: Crestal bone loss at the time of loading for Group 1 evaluated by CBCT was 0.950 ± 0.988 while after 24 weeks of loading, it was 1.388 ± 0.576. For Group 2, mean crestal bone loss was 1.200 ± 0.925 at the time of loading, and after 24 weeks, it was 1.512 ± 0.674. Crestal bone loss at the time of loading for Group 1 evaluated by DR was 1.075 ± 0.849 while after 24 weeks of loading, it was 1.562 ± 0.480. For Group 2, mean crestal bone loss was 1.162 ± 0.833 at the time of loading and after 24 weeks, it was 1.700 ± 0.498. CONCLUSIONS: In the present study, no statistically significant difference was observed in crestal bone loss between conventional and diode laser technique.

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The effect of exogenous microbial lipase enzyme on the ripening of Swiss cheese (0, 200, and 800U lipase in 30 L milk) was investigated for the physico-chemical, textural and sensory properties, along with its microstructure. The aim of this study was to investigate the application of microbial lipase to accelerate the ripening without affecting its original desirable quality characteristics. The effect of the microbial lipase was studied at different time periods (2, 30, 45, and 60 days) of the Swiss cheese ripening stages. Statistical analysis of the results showed that the physico-chemical parameters of cheese slightly increased during the ripening. Also, at all stages of the ripening hardness of Swiss cheese increased while the brittleness decreased. The number and size of the fat globules were also affected by the addition of the lipases. This study also showed that increase in the lipase amount had no significant change in quality and sensory parameters. Therefore, 200U of lipase was found to be sufficient to reduce the ripening time from 90 to 60 days by maintaining its genuine quality. Thus, this study suggested that the addition of microbial lipase may significantly reduce the cost of the cheese production by lowering the ripening period by 1 month and maintaining the quality of the final product.

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BACKGROUND/PURPOSE: The purpose of this study was to compare the shear bond strength of high strength ceramics to cut dentine as influenced by different resin cement types after thermocycling. MATERIALS AND METHODS: Shear bond strength testing was carried out for 56 sound, freshly extracted first permanent molars. Specimens were divided at random into 2 groups (n = 28) Lithium disilicate and Zirconia. Lithium disilicate and Zirconia specimens were further subdivided depending upon luting with Rely X ultimate cement bonded with single bond universal adhesive and Rely X U200 cement. Half of the specimens of each material luted with cements were subjected to thermocycling. Shear bond strength was evaluated using Universal testing machine at a crosshead speed of 5 mm/min. Results were compared and evaluated using t-test at a significance level of 0.05. The nature of bond failure was observed under a stereomicroscope for each sample. RESULTS: The mean difference of Lithium disilicate test specimens bonded with Rely X ultimate cement bonded with single bond universal adhesive with and without thermocycling was found to be 42.95+/-17.41MPa and 120.62+/-56.46 MPa respectively. The mean difference of Zirconia test specimens with Rely X ultimate cement bonded with single bond universal adhesive, with and without thermocycling, was found to be 8.74+/-2.90 MPa and 164.28+/-43.78 MPa respectively. The mean difference of Lithium disilicate test specimens bonded with Rely X U200 with and without thermocycling was found to be 2.36+/-0.63 MPa and 36.79.62+/-17.21MPa respectively. The mean difference of Zirconia test specimens bonded with Rely X U200 with and without thermocycling was found to be 5.96+/-3.11MPa and 122.46+/-23.01MPa respectively. CONCLUSION: Zirconia was found to have better shear bond strength than Lithium discilicate. The use of single bond universal adhesive improves bonding to newer higher strength ceramics such as Zirconia. Cohesive failure was predominant at cement dentine interface.

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AIM AND OBJECTIVES: The aim of the study was to assess the knowledge of dental laboratory technicians regarding infection control and modes of infection control employed by them. SETTINGS AND DESIGN: A self-assessment questionnaire-based survey was carried out among dental technicians to assess the knowledge and practice of infection control in dental laboratories. MATERIALS AND METHODS: Survey instrument containing 16 questions were randomly distributed to 70 dental colleges of North India regarding knowledge of infection control methods and infection control practised in laboratories. Data were collected and analyzed. RESULTS: The response showed that 30.76% of dental technicians receive 30-50 or more than 50 impressions in a week. About 96.15% of the technicians used a plastic bag to carry impressions. Twenty-five percent of the dental technicians were aware of infection control protocol. Fifty-five percent of the technicians received impressions while wearing gloves and 61.53% of the institutes had a separate receiving area. Nearly 71.15% of the technicians communicate with the doctor regarding the disinfection of impression received in the laboratory. Almost 30.76% of the dental technicians disinfect all the impressions and 67.30% technicians use immersion for disinfection of impressions. Only 38.46% responded that they immerse impressions for 10 min for disinfection. About 73.07% use gloves, 90.38% use mouth masks, 57.69% wear eye shields, and 88.46% wear aprons while working. Nearly 78.84% of the technicians received vaccination against hepatitis B virus. Almost 69.23% of the technicians change pumice slurry after regular intervals, and 75% do not add any disinfectant. Nearly 59.61% of technicians disinfect the prostheses before sending it to the clinic, and 42.30% disinfect them by immersion technique. About disposal of waste, 80.76% said that they dispose the waste properly. CONCLUSION: To summarize, most of the technicians were not aware of basic infection control protocols.

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Nutrition is important in every stage of child development. A child born with cleft lip and palate may experience difficulties while feeding due to the lack of seal of the oral cavity due to incomplete facial and palatal structures. Difficulty in feeding leads to inadequate nutrition and affects the health. Children with cleft lip and palate need certain modifications to thrive and grow. Feeding difficulties should be assessed and intervened as early as possible, as they are an important aspect of multidisciplinary team approach in management and may have an impact on long-term outcome. This case report presents fabrication of feeding appliance in 6-month-old infant with cleft palate with ethylene vinyl acetate.

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In maxillary defects, role of a prosthodontist is to rehabilitate the intra- and extra-oral structures and to endow with the normal function of mastication, speech, deglutition, and esthetics. Malignancies are common in the oral region and are treated usually through surgical intervention. Surgical intervention creates anatomical defects creating communication between oral and nasal cavity. Patients pose difficulties while performing normal functions such as swallowing and speaking, due to this communication. To overcome the problems encountered by the patient, obturators are fabricated. The main problem with rehabilitation of large defect is the weight of prosthesis; the prosthesis becomes very bulky and nonretentive due to its weight. Hollow obturators are fabricated to conquer this tribulation by different techniques. This case report describes a simplified method of fabrication of a definitive hollow bulb obturator for rehabilitation of a maxillary defect (Aramany's class I) by insertion of balloon.

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Digitalization has become part and parcel of contemporary prosthodontics with the probability of most of the procedures being based on the digital techniques in the near future. This digital revolution started in the latter half of the 20th century by converting analog objects/signals into digital bits and bytes. Recent developments in all-ceramic materials and systems of computer-aided designing and computer-aided manufacturing (CAD/CAM), copy milling, and so forth offer excellent esthetics and superb biocompatibility. Copy milling system for ceramics enables milling of the zirconia cores of all-ceramic restorations precisely and also if this system is properly used the procedure for fabricating all-ceramic restorations can be substantially simplified. This case report presents fabrication of all-ceramic Maryland Bridge and post-core with a copy milling system for esthetics and preservation of integrity of tooth. For both of the patients, the use of biologic, all-ceramic, copy-milled restorations resulted in clinical success and recovered function and esthetics.

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The aim of this manuscript was to evaluate the major technical problems on the isolation and characterization of the collagen from fish waste materials that were usually faced by the growing researchers. Although the original research article published by authors contributed new information to the literature, some of them were failed to provide sufficient details in order to reproduce the study as well as could not adequately interpret/compared the results with other publications. Therefore, it is required to research the technical problems during the isolation and characterization of the collagen. This technical note provides the information which is crucial for the reader's and growing researchers for understanding as an essential part of the published research studies about the collagen extraction and characterization. Hence, this technical note may be helpful to those working on the collagen extraction and characterization from fish/marine waste materials.

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AIM AND OBJECTIVES: The aim of the study was to evaluate the prevalence of temporomandibular disorder (TMD) by the use of Helkimo's index (anamnestic [Ai] and clinical dysfunction [Di] component) in the nonpatient population (dental students) of Faridabad college. SETTINGS AND DESIGN: A questionnaire-based survey was carried out among students of dental college for signs and symptoms of TMD and also clinical examination was done. The results were scored and according to scoring severity of TMD were assessed in the specified population. MATERIALS AND METHODS: About 580 students were assessed for TMD by the use of Helkimo's index (Ai and Di component). Descriptive statistical analysis was done. RESULTS: Among the study group, 15% were found to have TMDs. Out of the affected students, 79% females and 21% males were having symptoms. Out of the signs and symptoms present, 7% students were found to have sound in temporomandibular joint followed by pain in 3% and fatigue in 2% of students. On clinical examination, limited mouth opening was found in 6% students followed by locked mandible in 1%, deviation of jaw in 0.6%, and jaw rigidity of mandible in 0.6% of individual. CONCLUSION: To summarize, Helkimo index is a well-founded index to assess TMD in a specified population. Signs and symptoms of TMD were present among students although low prevalence of TMD was found in the students.

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At present, implant supported or retained prostheses are considered as a first therapeutic alternative for patients. Although the success rate of dental implants is very high, no treatment is without complications; same applies to implants also. Implant failure can be due to biological factors, i.e., loss of osseointegration or due to technical complications. This case report presents implant complications involving both factors along with the management of these cases. In implant supported overdenture patient, loss of implant on the right side and implant body fracture of the left side implant is reported and in another patient abutment screw fracture and its management is reported.

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BACKGROUND/PURPOSE: The aim of this study was to compare the shear bond strength of computer aided design/computer aided machined ceramic (CAD/CAM), pressable ceramic, and milled metal implant copings on abutment and the effect of surface conditioning on bonding strength. MATERIALS AND METHODS: A total of 90 test samples were fabricated on three titanium abutments. Among 90 test samples, 30 copings were fabricated by CAD/CAM, 30 by pressable, and 30 by milling of titanium metal. These 30 test samples in each group were further subdivided equally for surface treatment. Fifteen out of 30 test samples in each group were surface conditioned with airborne particle abrasion. All the 90 test samples were luted on abutment with glass ionomer cement. Bonding strength was evaluated for all the samples using universal testing machine at a crosshead speed of 5 mm/min. The results obtained were compared and evaluated using one-way ANOVA with post-hoc and unpaired t-test at a significance level of 0.05. RESULTS: The mean difference for CAD/CAM surface conditioned subgroup was 1.28 ± 0.12, for nonconditioned subgroup was 1.20 ± 0.11. The mean difference for pressable surface conditioned subgroup was 1.18 ± 0.04, and for nonconditioned subgroup was 0.75 ± 0.28. The mean difference for milled metal surface conditioned subgroup was 2.57 ± 0.58, and for nonconditioned subgroup was 1.49 ± 0.15. CONCLUSIONS: On comparison of bonding strength, milled metal copings had an edge over the other two materials, and surface conditioning increased the bond strength.