RESUMEN
A simple and efficient procedure was employed for the synthesis of N'-(1,4-naphtho-quinone-2-yl) isonicotinohydrazide (NIH) by the reaction of 2-hydroxy-1,4-naphthaquinone (lawsone) and isonicotinoyl hydrazine in methanol using ultrasonic irradiation. Lawsone is the principal dye, isolated from the leaves of henna (Lawsonia inermis). Structural modification was done on the molecule aiming to get a more active derivative. The structure of the parent compound and the derivative was characterized by elemental analyses, infrared, electronic, (1)H, (13)C NMR and GC-MS spectra. The fluorescence spectral investigation of the compound was studied in DMSO and ethanol. Single crystal X-ray diffraction studies reveal that NIH crystallizes in monoclinic space group. The DNA cleavage study was monitored by gel electrophoresis method. The synthesized compound was found to have significant antioxidant activity against DPPH radical (IC50=58 µM). The in vitro cytotoxic studies of the derivative against two human cancer cell lines MCF-7 (human breast cancer) and HCT-15 (human colon carcinoma cells) using MTT assay revealed that the compound exhibited higher cytotoxic activity with a lower IC50 value indicating its efficiency in killing the cancer cells even at low concentrations. These results suggest that the structural modifications performed on lawsone could be considered a good strategy to obtain a more active drug.
Asunto(s)
Hidrazinas/química , Hidrazinas/síntesis química , Hidrazinas/farmacología , Isoniazida/análogos & derivados , Ácido Ascórbico/química , Compuestos de Bifenilo/química , Espectroscopía de Resonancia Magnética con Carbono-13 , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Cristalografía por Rayos X , División del ADN/efectos de los fármacos , Electroforesis en Gel de Agar , Depuradores de Radicales Libres/química , Cromatografía de Gases y Espectrometría de Masas , Humanos , Hidrazinas/toxicidad , Enlace de Hidrógeno , Isoniazida/síntesis química , Isoniazida/química , Isoniazida/farmacología , Conformación Molecular , Picratos/química , Espectroscopía de Protones por Resonancia Magnética , Estándares de Referencia , Espectrometría de Fluorescencia , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de FourierAsunto(s)
Gingivitis/diagnóstico , Granuloma Piogénico/diagnóstico , Complicaciones del Embarazo/diagnóstico , Lengua , Placa Dental/diagnóstico , Placa Dental/terapia , Femenino , Gingivitis/terapia , Granuloma Piogénico/terapia , Humanos , Embarazo , Complicaciones del Embarazo/terapia , Índice de Severidad de la Enfermedad , Adulto JovenRESUMEN
Advanced abdominal pregnancy is a rare event with a high fetal and maternal morbidity and mortality. We present 3 cases of abdominal pregnancy seen at our hospital. The perinatal mortality was 100% with no maternal deaths. Literature has been reviewed and management of abdominal pregnancy is discussed. Awareness of this condition and its clinical features is important in preventing abdominal pregnancy related morbidity, mortality. Careful clinical examination coupled with investigatory aids like X-rays and ultrasound will clinch the diagnosis. Most of the patients require early laparotomy following diagnosis and the morbidity and mortality are high particularly for the fetus.
Asunto(s)
Muerte Fetal , Embarazo Abdominal , Adulto , Femenino , Humanos , Embarazo , Embarazo Abdominal/diagnósticoRESUMEN
Twenty-seven cases of endometriosis in abdominal scars seen over a 10-year period have been reviewed. Multiparous women within the age group of 25-35 years were affected more frequently. Hysterotomy for termination of midterm pregnancy was the most common surgical procedure done prior to the occurrence of scar endometriosis. Associated pelvic endometriosis was present in 25.9% of the cases. Treatment of choice is complete surgical excision. Progestogen therapy appears to be less effective. Good technique and proper care during surgery may help in preventing this complication.