RESUMEN
O objetivo deste estudo foi verificar a oferta da disciplina de Bioética nos currículos dos cursosde odontologia brasileiros.Para isso foi realizado a revisão de todas as matrizes curriculares disponíveis no site do Ministério de Educação, no período de fevereiro a abril de 2019. A busca foi realizada individualmente na matriz de cada curso buscando informações sobre: a presença da disciplina de Bioética, a carga horária da disciplina, o semestre e o regime (optativa ou obrigatória) de oferta na matriz curricular. No levantamento realizado encontrou-se 41 (17,67%) cursos que ofertavam a disciplina de Bioética de forma isolada e 36 (15,52%) na forma associada, totalizando 77 (33,19%) com oferta da disciplina. Concluiu-se que, no ano de 2019, a maioria dos cursos não disponibilizava a disciplina de Bioética, inferindo-se a necessidade de haver sua inclusão em todos os cursos como forma de cumprir as diretrizes curriculares nacionais existentes e proporcionar adequada formação humanística aos futuros profissionais (AU).
El objetivo de este estudio fue verificar la oferta de la asignatura de Bioética en los planes de estudio de los cursosde odontología brasileños. Para eso fue realizado una revisión de todas las matrices curriculares disponibles en el sitio del Ministerio de Educación de Brasil, de febrero a abril de 2019. La búsqueda fue realizada de forma individualizada en la matriz de cada curso buscando las informaciones sobre: presencia de la asignatura de Bioética, la carga horaria de la asignatura, el semestre y el régimen (obligatoria u optativa) que era ofertada. En la búsqueda encontramos que 41 (17,67%) cursos ofrecían la asignatura de Bioética de forma aislada y 36 (15,52%) en forma asociada, totalizando 77 (33,19%) con oferta de la asignatura. Se concluyó que, en 2019, la mayoría de los cursos no proporcionaban la asignatura de Bioética, infiriendo la necesidad de ser incluida en todos los cursos como una forma de cumplir con las directrices curriculares existentes y proporcionar capacitación humanística adecuada para los futuros profesionales (AU).
The aim of this study was to verify the offer of the Bioethics course in the curricular matrices of Brazilian dentistry schools. For this purpose, a review of all curricular matrices available on the Ministry of Education website was conducted from February to April 2019. The search was carried out individually in the matrix of each course, seeking information about the presence of the Bioethics course, the course load, the semester, and the regime (optional or mandatory) of the course offering in the curricular matrix. In the survey, 41 (17.67%) courses were found to offer the Bioethics course singly, and 36 (15.52%) offered it in the associated form, totaling 77 (33.19%) with an offer of the courses. It was concluded that, in 2019, the majority of courses did not provide the Bioethics subject, inferring the need for its inclusion in all courses as a way to comply withthe existing national curricular guidelines and provide adequate humanistic education to future professionals (AU).
Asunto(s)
Bioética/educación , Educación en Odontología , Ética Odontológica/educación , Encuestas y Cuestionarios , Análisis de DocumentosRESUMEN
The aim of this case series was to evaluate the effects of blue®m mouthwash on oral surgical wounds. Eleven patients underwent bilateral preprosthetic surgery and were instructed to apply the product only to the right side of the surgery. In this way, the right side corresponds to the test side and the left side (place without applying any type of solution) to the control side. After seven days of using the product (3 times a day), the following parameters were evaluated by means of a visual analogue scale: pain, changes in taste, and acceptance by the patient. Then, the level of tissue inflammation was assessed, by the number of pixels, using ImageJ® software. The main results show that the blue®m mouthwash was widely accepted by patients, reducing their pain. The number of inflammation pixels was lower on the test side (p < 0.05), indicating improved healing. It is suggested that blue®m mouthwash positively influences tissue healing reducing pain and the postsurgical inflammatory process; however, randomized clinical trials should be done to prove this clinical observation.
RESUMEN
OBJECTIVES: To analyze the inflammatory millieu in oral squamous cell carcinoma (OSCC) tumors and the influence of macrophages related-cytokines on the tumor cell migration. MATERIALS AND METHODS: Inflammatory protein profile and macrophage population (M2/M1 ratio) of human OSCC fragments were analyzed by proteomic analysis and flow cytometry assay respectively. To evaluate the effects of inflammation on OSCC behavior, we analyzed the role of polarized macrophages and cytokines (IL-6, IL-1ß and TNF-α) on OSCC cell lines (SCC25 and Cal27) responsiveness by western blotting (cell signaling) and time-lapse (cell migration). Also, it was addressed the crosstalk of IL-6-STAT3 axis with cell migration signaling using a STAT3 inhibitor (Stattic®) and a pull down assay for the RhoGTPase Rac1 activity. RESULTS: It was observed a ~2 fold predominance of M2 over M1 macrophages and a pro-inflammatory state in OSCC fragments. The M2 conditioned media increased migration speed and directionality of highly invasive OSCC cells (SCC25). OSCC cell lines were responsive to cytokine stimuli (IL6, IL-1ß and TNF-α), but only IL-6 increased migration properties of OSCC cells. This effect was dependent on STAT3-phosphorylation levels, which interfered with Rac1 activation levels. CONCLUSION: Our results suggest that the inflammatory milieu might favor invasion and metastasis of OSCC by the direct effect of macrophage-related cytokines on tumor migration.
Asunto(s)
Movimiento Celular , Citocinas/metabolismo , Neoplasias de la Boca , Carcinoma de Células Escamosas de Cabeza y Cuello , Microambiente Tumoral , Macrófagos Asociados a Tumores , Análisis de Varianza , Cadherinas/metabolismo , Comunicación Celular , Línea Celular Tumoral , Forma de la Célula , Medios de Cultivo Condicionados/farmacología , Citometría de Flujo , Humanos , Inflamación , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Neoplasias de la Boca/metabolismo , Neoplasias de la Boca/patología , Invasividad Neoplásica , Fosforilación , Proteómica , Factor de Transcripción STAT3/antagonistas & inhibidores , Factor de Transcripción STAT3/metabolismo , Carcinoma de Células Escamosas de Cabeza y Cuello/metabolismo , Carcinoma de Células Escamosas de Cabeza y Cuello/patología , Factor de Necrosis Tumoral alfa/metabolismo , Macrófagos Asociados a Tumores/citología , Macrófagos Asociados a Tumores/metabolismo , Macrófagos Asociados a Tumores/fisiología , Proteína de Unión al GTP rac1/metabolismoRESUMEN
Objetivo: O objetivo deste estudo foi revisar sistematicamente na literatura publicações relacionadas ao protocolo de atendimento do cirurgião-dentista frente a pacientes com fissura labiopalatal. Métodos: Foram revisados artigos publicados até março de 2018, nas línguas inglesa, portuguesa e espanhola, obtidos por meio de buscas nas bases eletrônicas PubMed, Scopus, LILACS, Bireme, SciELO e busca manual nas referências dos artigos encontrados. Resultados: A busca resultou em 141 artigos, onde após a aplicação dos critérios de exclusão foram obtidos quatro artigos. Os artigos selecionados eram de revisão de literatura, o que impossibilitou a realização de uma revisão sistemática da literatura. Todos os artigos foram lidos na íntegra e resultaram em um protocolo de atendimento dividido de acordo com as fases de erupção dental, com ênfase na prevenção. O protocolo foi separado em: fase pré-eruptiva e de dentição decídua, dentadura mista e dentição permanente. Na fase pré-eruptiva e de dentição decídua a orientação é dar ênfase na prevenção, na fase de dentadura mista tem-se o início da ortodontia preventiva além da manutenção das ações preventivas e na fase de dentição permanente trabalha-se novamente com a prevenção inicia-se a ortodontia fixa e quando necessário a realização de cirurgia ortognática. Conclusão: Em relação aos achados, fica evidente a importância da prevenção durante todas as fases, visando a manutenção do perímetro da arcada, e manutenção da integridade dos dentes permanentes. O cirurgião-dentista deve estar apto a realizar o atendimento tendo como base um protocolo para efetuar o manejo correto conforme a faixa etária e a fase de erupção dental em que o paciente se encontra. O aprimoramento do cirurgião-dentista na abordagem aos pacientes portadores de fissura labiopalatal deve ser constante, bem como a criação de vínculo afetivo com o paciente e a família visando um tratamento preciso e humanizado. (AU)
Aim: This study sought to systematically review publications in the literature related to the Dentist's Protocol as regards palatal cleft patients. Methods:This study reviewed articles published up to March 2018, in English, Portuguese, and Spanish, obtained through data searches on PubMed, Scopus, LILACS, Bireme, SciELO, as well as through a manual search in the references of the identified articles. Results: The search resulted in 141 articles; after the application of the exclusion criteria, four articles were selected. The selected articles were literature reviews, making it impossible to conduct a systematic review of the literature. All of the obtained articles were read in full, resulting in a care protocol that was divided according to the dental eruption stages, with emphasis on prevention. The protocol was divided into: the pre-eruptive and deciduous dentition stage, mixed denture, and permanent dentition. In the pre-eruptive and deciduous dentition stage, the dental advice is to emphasize prevention; in the mixed dentition stage, there is the beginning of preventive orthodontics as well as the maintenance of preventive actions; while in the permanent dentition stage, one again works with prevention. fixed orthodontics is begun, and when necessary, orthognathic surgery is also applied. Conclusion: Regarding the findings, the importance of prevention during all stages is clear, in an attempt maintain the perimeter of the dental arch and the integrity of the permanent teeth. The dentist should be able to perform the service based on a protocol for the correct handling according to the age range and dental eruption stage of the patient. The improvement of the dentist in providing dental care to patients with cleft lip and palate should be constant, as should the creation of an affective bond with the patient and the family, aimed at providing a precise and humanized treatment. (AU)
Asunto(s)
Hueso Paladar/anomalías , Protocolos Clínicos , Labio Leporino , Fisura del Paladar , Atención Odontológica , OdontólogosRESUMEN
Carcinoma cuniculatum (CC) is a rare variant of squamous cell carcinoma (SCC). Only 27 cases have been published in English. A 50-year-old male, who presented a white nodule with erythematous areas, localized in the lateral border of the tongue with 2 months of duration. The patient presents oral lichen planus lesions on the tongue, commissure, and buccal mucosa. The microscopy evaluation of the nodular lesion of the tongue revealed a malignant epithelial neoplasia characterized by cuniculatum architecture, similar in appearance to "rabbit burrows" and the final diagnosis was of CC. The management of CC needs cooperation between surgeons and pathologists to establish a correct diagnosis and treatment. CC is a rare entity and must be recognized by oral pathologist so that it is not misdiagnosed as verrucous carcinoma or oral SCC (OSCC). Regarding prognosis, CC must be evaluated and distinguished from other variants of OSCC.
Asunto(s)
Carcinoma de Células Escamosas/complicaciones , Carcinoma de Células Escamosas/patología , Liquen Plano Oral/complicaciones , Liquen Plano Oral/patología , Neoplasias de la Lengua/complicaciones , Neoplasias de la Lengua/patología , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/ultraestructura , Diagnóstico Diferencial , Errores Diagnósticos/prevención & control , Humanos , Liquen Plano Oral/diagnóstico , Masculino , Microscopía , Persona de Mediana Edad , Neoplasias de la Lengua/diagnóstico , Neoplasias de la Lengua/ultraestructuraRESUMEN
BACKGROUND INFORMATION: Cell migration requires the coordinated activation of structural and signalling molecules, such as the RhoGTPase Rac1. It is known that the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex assembly, which generates reactive oxygen species (ROS) at the cell membrane, also relies on Rac1 activation, indicating a possible effect of ROS during cell migration. In this study, we evaluated the effect of NADPH-oxidase-derived ROS on the migration process. RESULTS: Using time-lapse videos of CHO.K1 cells plated on fibronectin (2 µg/ml) or collagen (5 µg/cm2 ), we observed that depletion of ROS by N-acetyl-cysteine (NAC, 10 mM), an unspecific antioxidant, or diphenyliodonium (DPI, 10 µM), a NADPH-oxidase inhibitor, induced a â¼50% decrease in migration speed and severely impacted migration directionality. Then, we analysed the effects of NADPH oxidase on three migratory events: protrusion rate, adhesion process and signalling pathways related to cell migration. DPI induced an increase of â¼3 protrusion/cell, which were 2× faster but had a â¼50% retraction when compared with control. By pull-down assay, we observed no changes on Rac1 activation, indicating that ROS-mediated effects were related to downstream molecules, such as adhesion-related molecules. A reduction of the adhesion marker FAK-Y397 levels in cells treated with NAC and DPI was observed. In order to analyse adhesion dynamics, CHO.K1 cells transfected with paxillin-GFP analysed with total internal reflectance fluorescence (TIRF) indicated that DPI (5 µM) induced larger adhesions when compared with control. CONCLUSION: These results indicate that the local generation of NADPH-oxidase-derived ROS can modulate cell migration due to changes on adhesion dynamics and signalling. SIGNIFICANCE: This study highlights the physiological requirement of ROS for cell migration and the potential use of these molecules as targets to modulate the cell migration process at different diseases.
Asunto(s)
Adhesión Celular , Movimiento Celular , NADPH Oxidasas/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Animales , Compuestos de Bifenilo , Células CHO , Adhesión Celular/efectos de los fármacos , Movimiento Celular/efectos de los fármacos , Cricetulus , Compuestos Onio , Transducción de Señal/efectos de los fármacosRESUMEN
Cell migration is regulated by adhesion to the extracellular matrix (ECM) through integrins and activation of small RhoGTPases, such as RhoA and Rac1, resulting in changes to actomyosin organization. During invasion, epithelial-derived tumor cells switch from laminin-enriched basal membrane to collagen and fibronectin-enriched connective tissue. How this switch affects the tumor migration is still unclear. We tested the hypothesis that ECM dictates the invasiveness of Oral Squamous Cell Carcinoma (OSCC). We analyzed the migratory properties of two OSCC lines, a low invasive cell line with high e-cadherin levels (Linv/HE-cad) or a highly invasive cell line with low e-cadherin levels (Hinv/LE-cad), plated on different ECM components. Compared to laminin, fibronectin induced non-directional collective migration and decreased RhoA activity in Linv/HE-cad OSCC. For Hinv/LE-cad OSCC, fibronectin increased Rac1 activity and induced smaller adhesions, resulting in a fast single cell migration in both 2D and 3D environments. Consistent with these observations, human OSCC biopsies exhibited similar changes in cell-ECM adhesion distribution at the invasive front of the tumor, where cells encounter fibronectin. Our results indicate that ECM composition might induce a switch from collective to single cell migration according to tumor invasiveness due to changes in cell-ECM adhesion and the resulting signaling pathways that alter actomyosin organization.
Asunto(s)
Carcinoma de Células Escamosas/patología , Adhesión Celular/efectos de los fármacos , Movimiento Celular/efectos de los fármacos , Fibronectinas/farmacología , Neoplasias de la Boca/patología , Transducción de Señal/efectos de los fármacos , Cadherinas/metabolismo , Carcinoma de Células Escamosas/metabolismo , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Humanos , Neoplasias de la Boca/metabolismoRESUMEN
Tufted angioma (TA) is a benign vascular tumor with endothelial origin. It is extremely rare in oral mucosa; only seven cases have been reported in the literature so far. Here, we describe two cases of tufted angioma observed in children and we also present a review of the literature about this pathology, concerning the differential diagnosis and management of this lesion in children.
RESUMEN
The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.
Asunto(s)
Ameloblastoma/patología , Neoplasias Maxilomandibulares/patología , Tumores Odontogénicos/patología , Biopsia con Aguja Fina , Citodiagnóstico/métodos , Células Epiteliales/patología , Eritrocitos/patología , Humanos , Inmunohistoquímica , Queratinas/inmunología , Adhesión en ParafinaRESUMEN
Odontogenic tumors (OTs) are lesions that develop exclusively on maxillary bones, and form a heterogeneous group. They vary from hamartomatous lesions to benign and malign tumors. Although they are rarely observed in dentistry clinics, it is extremely important for the dentist to be aware of them. The aim of this study was to investigate the incidence of odontogenic tumors diagnosed in the population of Florianópolis, Santa Catarina, Brazil. Cases of odontogenic tumors were selected from the anatomopathological diagnostic services at Federal University of Santa Catarina from 1998 to 2011. Clinical data on these cases were collected from biopsy reports and patient files. Seventy-eight cases of odontogenic tumors were surveyed. Of these diagnoses, 51% were keratocystic odontogenic tumors (KCOTs); the remaining cases were mainly ameloblastomas and odontomas. The most frequently observed lesion in this retrospective study was KCOT (more than half of cases). Thus, this study shows that modifying the classification of the OTs altered the frequency of the lesions, possibly making KCOT the most common lesion observed in diagnostic services worldwide.
Asunto(s)
Neoplasias Maxilomandibulares/epidemiología , Tumores Odontogénicos/epidemiología , Adolescente , Adulto , Distribución por Edad , Anciano , Brasil/epidemiología , Niño , Preescolar , Femenino , Humanos , Neoplasias Maxilomandibulares/patología , Masculino , Persona de Mediana Edad , Tumores Odontogénicos/patología , Prevalencia , Estudios Retrospectivos , Distribución por Sexo , Adulto JovenRESUMEN
BACKGROUND: Keratocystic odontogenic tumor (KCOT) is a benign tumor that arises sporadically or associated with nevoid basal cell carcinoma syndrome (NBCCS). Its locally aggressive behavior contrasts with its cystic histological appearance. To better understand the interaction between tumor cells and the stroma, the present study aimed to evaluate and compare the immunohistochemical expression of matrix metalloproteinases (MMP-1, -2, and -9), the cellular proliferation index (Ki-67), and the presence of myofibroblasts (MFs) in KCOTs. METHODS: Eleven cases of isolated KCOT (G1) and 12 cases of KCOT associated with NBCCS (G2) were selected for an immunohistochemical investigation of the proteins MMP-1, MMP-2, MMP-9, Ki-67, and α-smooth muscle actin (α-SMA) in MFs. A group of 6 pericoronal follicles (G3) was included as a normal odontogenic tissue control. RESULTS: Significant differences between the G3 and G1/G2 groups regarding the expression of MMP-1, MMP-9 (in connective tissue), and Ki-67 were observed. In KCOT, there was a positive correlation between the Ki-67 antigen and MMP-1 and between MFs and MMP-1 in the parenchyma. No statistical differences were found between G1 and G2 groups. CONCLUSIONS: MMP-1, MMP-9, and proliferative activity appear to play important roles in KCOT pathogenesis. The increased proliferative activity with KCOT was associated with elevated MMP-1 production in the parenchyma, which influenced the growth of the lesion in association with an increased number of MFs.
Asunto(s)
Saco Dental/química , Antígeno Ki-67/análisis , Metaloproteinasa 1 de la Matriz/análisis , Metaloproteinasa 2 de la Matriz/análisis , Metaloproteinasa 9 de la Matriz/análisis , Miofibroblastos/patología , Tumores Odontogénicos/química , Actinas/análisis , Síndrome del Nevo Basocelular/metabolismo , Síndrome del Nevo Basocelular/patología , Proliferación Celular , Tejido Conectivo/química , Tejido Conectivo/patología , Saco Dental/patología , Epitelio/química , Epitelio/patología , Humanos , Inmunohistoquímica , Queratinas/análisis , Tumores Odontogénicos/patología , Células del Estroma/química , Células del Estroma/patologíaRESUMEN
In most cases involving jaw lesions, a biopsy and a histopathological analysis are necessary to establish the final diagnosis. However, biopsy may be a complex procedure at some maxillomandibular sites, and some systemic conditions could contraindicate the procedure. Thus, a search for new, less invasive techniques, which could eventually replace biopsy and simplify the diagnostic process, would benefit both professionals and patients. The aim of this study was to evaluate the cell block technique, prepared from the aspiration of luminal contents, in the preoperative diagnosis of keratocystic odontogenic tumors (KCOT). From 135 cases of lesions aspirated and processed by the cell block technique, we selected those containing keratin. In all cases selected, histological diagnosis was based on surgical biopsy. From 20 cases containing keratin in the cytological analyses, 19 were KCOTs and one was an orthokeratinized odontogenic cyst (OOC). In all KCOT cases, we observed the presence of parakeratin, even in those with intense inflammation. In the cytological analysis of the OOC, parakeratin was not observed. In conclusion, there is strong evidence that KCOT can be confidently diagnosed preoperatively by cytological analyses of lesions punctured and processed by the cell block technique.
Asunto(s)
Técnicas Citológicas , Neoplasias Maxilomandibulares/diagnóstico , Tumores Odontogénicos/diagnóstico , Biopsia con Aguja , HumanosRESUMEN
Odontogenic tumors (OTs) are lesions that develop exclusively on maxillary bones, and form a heterogeneous group. They vary from hamartomatous lesions to benign and malign tumors. Although they are rarely observed in dentistry clinics, it is extremely important for the dentist to be aware of them. The aim of this study was to investigate the incidence of odontogenic tumors diagnosed in the population of Florianópolis, Santa Catarina, Brazil. Cases of odontogenic tumors were selected from the anatomopathological diagnostic services at Federal University of Santa Catarina from 1998 to 2011. Clinical data on these cases were collected from biopsy reports and patient files. Seventy-eight cases of odontogenic tumors were surveyed. Of these diagnoses, 51% were keratocystic odontogenic tumors (KCOTs); the remaining cases were mainly ameloblastomas and odontomas. The most frequently observed lesion in this retrospective study was KCOT (more than half of cases). Thus, this study shows that modifying the classification of the OTs altered the frequency of the lesions, possibly making KCOT the most common lesion observed in diagnostic services worldwide.
Asunto(s)
Adolescente , Adulto , Anciano , Niño , Preescolar , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Neoplasias Maxilomandibulares/epidemiología , Tumores Odontogénicos/epidemiología , Distribución por Edad , Brasil/epidemiología , Neoplasias Maxilomandibulares/patología , Tumores Odontogénicos/patología , Prevalencia , Estudios Retrospectivos , Distribución por SexoRESUMEN
INTRODUÇÃO: A busca por novos métodos que auxiliem e simplifiquem de maneira eficaz o diagnóstico de lesões maxilares, cistos e tumores, objetiva beneficiar os pacientes e facilitar a atuação dos profissionais da área de diagnóstico bucal. Além dos dados clínicos, radiográficos e histopatológicos classicamente utilizados nos protocolos de investigação das lesões maxilares, a adaptação de técnicas já consagradas na medicina pode ser de grande valia. A técnica de cell block se propõe a auxiliar nesse processo, pois consiste na análise citológica de materiais, efusões e líquidos, coletados de lesões passíveis de punção aspirativa, como cistos e tumores císticos dos maxilares. OBJETIVO: Demonstrar a aplicabilidade da técnica de cell block para avaliação citológica de material biológico coletado a partir de lesões císticas dos maxilares. RESULTADOS: Das 20 lesões, das quais o conteúdo foi processado pela técnica, a avaliação citológica de cinco casos de tumores odontogênicos ceratocísticos (TOCs) demonstrou a presença predominante de ceratina, sempre com áreas de paraceratina. Nos demais casos (cinco cistos dentígeros, cinco cistos radiculares e cinco cistos residuais) foi observada a presença de hemácias, células inflamatórias e fendas de cristais de colesterol. CONCLUSÃO: A avaliação citológica, a partir da técnica de cell block, foi útil no estabelecimento do diagnóstico diferencial entre TOC e demais lesões estudadas, cistos radicular, residual e dentígero.
INTRODUCTION: The search for new methods that aid and optimize the diagnosis of cystic and tumoral maxillary lesions aims to benefit both patients and professionals from oral diagnosis. In addition to clinical, radiographic and histological findings traditionally used in research protocol for maxillary lesions, the adaptation of widely used medical techniques may be very helpful. The cell block procedure streamlines this process, inasmuch as it consists of cytopathological analysis of materials, liquids and effusions, collected from aspirated lesions such as maxillary cysts and cystic maxillary tumors. OBJECTIVE: To demonstrate the applicability of cell block technique for cytological assessment of biological material collected from cystic maxillary lesions. RESULTS: The cytological evaluation revealed that five out of 20 lesions, whose content had been processed by this technique, were odontogenic keratocystic tumors (TOCs), predominantly with keratin and always with parakeratin areas. In the other cases (five dentigerous, five radicular and five residual cysts) there were erythrocytes, inflammatory cells, and cholesterol clefts. CONCLUSION: The cytological assessment through cell block technique was useful as to establishing the differential diagnosis between TOC and other lesions, radicular, residual and dentigerous cysts.
Asunto(s)
Humanos , Masculino , Femenino , Adulto Joven , Persona de Mediana Edad , Biopsia con Aguja , Quiste Dentígero/diagnóstico , Quiste Radicular/diagnóstico , Quistes Odontogénicos/diagnóstico , Diagnóstico Diferencial , Técnicas y Procedimientos Diagnósticos , Tumores Odontogénicos/diagnósticoRESUMEN
The development of the oral squamous cell carcinoma (OSCC) is a multistep process that requires the accumulation of multiple genetic alterations usually preceded by detectable mucosal changes, most often leukoplakias and erythroplakias. The clinical appearance of oral precancerous lesions and their degree of epithelium dysplasia suggests the malignization potential. Several techniques have been developed to improve the clinical and cytological diagnosis of oral precancerous lesions. The present paper reviews the main techniques used to improve premalignant lesion diagnosis.