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INTRODUCTION: A third of hypertensive patients have major depression, a relationship that is associated with a worse prognosis. The objective of the study was to estimate the association between depression and high blood pressure, as well as to establish the possible bidirectionality of the conditions. MATERIALS AND METHODS: Retrospective cohort study. People between 18 and 65 years old with high blood pressure, depression or use of medications for their management were included. To analyze the antecedent, a comorbidity model was performed. A bivariate analysis was performed and then a multivariate logistic regression. The association was estimated using the Chi-square test and the odds ratios that were crude and adjusted to the other variables included in the analysis. The Hosmer-Lemeshow test was used to assess the goodness of fit. SPSS® v.21 was used as the statistical package. RESULTS: A total of 1,721 people were included in the study. The prevalence of depression in patients with and without hypertension was 17.4 and 12.6%, respectively, with a 43% risk of hypertension in people with depression. In patients with depression, it preceded the diagnosis of hypertension in 64.8% of cases and in hypertensive patients, 22.2% were later diagnosed with depression. The association between high blood pressure and major depression remained significant after adjusting for the other risk factors. CONCLUSIONS: Depression was found as a risk factor for high blood pressure, with a 2-way risk relationship between depression and high blood pressure.
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Trastorno Depresivo Mayor/epidemiología , Hipertensión/epidemiología , Adolescente , Adulto , Anciano , Estudios de Cohortes , Colombia , Trastorno Depresivo Mayor/complicaciones , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de Riesgo , Adulto JovenRESUMEN
The goal of this study was to evaluate whether a maternal energy - restricted diet could program ovarian angiogenesis and growth factor and gonadotropin receptors expression in rats . After parturition, s ix Wistar rats with 6 pups each were random ly assigned to one of the following groups: control group (C), with free access to a standard laboratory diet containing 23% protein; and energy - restricted gr oup (ER), receiving 60% of the amount consumed by the control group. After weaning, all female pup s had free access to the standard laboratory diet until 90 days of age, when they were sacrificed during the proestru s stage . Blood was collected for further evaluation of serum estradiol levels . One ovary was stored at - 80ºC for RT - PCR analysis while the other one was paraffin embedded, sectioned at 5 - μm thickness and processed by routine histological analys i s for evaluation of vessel density . The ER group had a reduction in follicle stimulating hormone (C = 2.36 ± 0.21, ER = 0.97 ± 0.15, P < 0.001) and lu teinizing hormone ( C = 1.81 ± 0.11, ER = 0.74 ± 0.11, P < 0.0004) receptor expression . This group also had an increase in gene expression of basic fibroblast growth (C = 0.51 ± 0.07, ER = 0.72 ± 0.06 , P < 0.04) and vascular endothelial growth factors (C = 0.82 ± 0.03, ER = 1.06 ± 0.08, P < 0.02). E stradiol serum concentration, vessel density, kinase - insert domain receptor, Fms - like tyrosine kinase - 1 and fibroblast growth factor receptor - 1 gene expressions were unchanged. The results indicated that a materna l energy - restricted diet during lactation programs the ovarian response to FSH, LH and angiogenic factors.
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Animales , Estradiol/biosíntesis , Fibroblastos/citología , Folículo Ovárico/anatomía & histología , Dieta , Ratas WistarRESUMEN
The goal of this study was to evaluate whether a maternal energy - restricted diet could program ovarian angiogenesis and growth factor and gonadotropin receptors expression in rats . After parturition, s ix Wistar rats with 6 pups each were random ly assigned to one of the following groups: control group (C), with free access to a standard laboratory diet containing 23% protein; and energy - restricted gr oup (ER), receiving 60% of the amount consumed by the control group. After weaning, all female pup s had free access to the standard laboratory diet until 90 days of age, when they were sacrificed during the proestru s stage . Blood was collected for further evaluation of serum estradiol levels . One ovary was stored at - 80ºC for RT - PCR analysis while the other one was paraffin embedded, sectioned at 5 - μm thickness and processed by routine histological analys i s for evaluation of vessel density . The ER group had a reduction in follicle stimulating hormone (C = 2.36 ± 0.21, ER = 0.97 ± 0.15, P < 0.001) and lu teinizing hormone ( C = 1.81 ± 0.11, ER = 0.74 ± 0.11, P < 0.0004) receptor expression . This group also had an increase in gene expression of basic fibroblast growth (C = 0.51 ± 0.07, ER = 0.72 ± 0.06 , P < 0.04) and vascular endothelial growth factors (C = 0.82 ± 0.03, ER = 1.06 ± 0.08, P < 0.02). E stradiol serum concentration, vessel density, kinase - insert domain receptor, Fms - like tyrosine kinase - 1 and fibroblast growth factor receptor - 1 gene expressions were unchanged. The results indicated that a materna l energy - restricted diet during lactation programs the ovarian response to FSH, LH and angiogenic factors.(AU)
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Animales , Folículo Ovárico/anatomía & histología , Estradiol/biosíntesis , Fibroblastos/citología , Dieta , Ratas WistarRESUMEN
The percent of lipids in the western diet has been continuously increasing in the last decades and is associated with a decrease in the proportion of protein intake. Recently, we demonstrated that protein malnutrition during lactation is associated with lower body weight and thyroid hypofunction in female rats and their offspring. Our objective in the present study was to determine if a high-fat and low-protein diet was associated with similar changes. Three-month-old female Wistar rats were randomly assigned to one of the following groups with 8 animals each: high-fat and low-protein (40% lipid, 5% protein, and 55% carbohydrate of the total energy content) from the 3rd week of gestation to the end of lactation; control group--standard diet (11% lipid, 23% protein, and 66% carbohydrate of the total energy content). Food consumption and body weight were monitored daily. Serum thyrotropin and thyroid hormone concentrations were determined by specific radioimmunoassay at the end of lactation. Animals receiving high-fat and low-protein diet had a significantly lower body weight (13.9% at weaning, P < 0.05) and serum albumin (25%, P < 0.05) and thyrotropin (26.2%, P < 0.01) concentrations, and a higher serum triiodothyronine concentration (74%, P < 0.005) and 131I-thyroid uptake (77%, P < 0.005). These data show that a high-fat and low-protein diet can promote maternal thyroid hyperfunction that differs from the thyroid hypofunction observed in dams fed a low-protein diet, a phenomenon that can be of adaptive importance for pup nurturing.
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Adaptación Fisiológica/fisiología , Dieta con Restricción de Proteínas , Grasas de la Dieta/metabolismo , Lactancia/metabolismo , Glándula Tiroides/fisiología , Hormonas Tiroideas/análisis , Animales , Peso Corporal , Ingestión de Energía , Femenino , Masculino , Embarazo , Radioinmunoensayo , Ratas , Ratas Wistar , Albúmina Sérica/análisis , Pruebas de Función de la Tiroides , Glándula Tiroides/metabolismo , Hormonas Tiroideas/metabolismoRESUMEN
The percent of lipids in the western diet has been continuously increasing in the last decades and is associated with a decrease in the proportion of protein intake. Recently, we demonstrated that protein malnutrition during lactation is associated with lower body weight and thyroid hypofunction in female rats and their offspring. Our objective in the present study was to determine if a high-fat and low-protein diet was associated with similar changes. Three-month-old female Wistar rats were randomly assigned to one of the following groups with 8 animals each: high-fat and low-protein (40% lipid, 5% protein, and 55% carbohydrate of the total energy content) from the 3rd week of gestation to the end of lactation; control group - standard diet (11% lipid, 23% protein, and 66% carbohydrate of the total energy content). Food consumption and body weight were monitored daily. Serum thyrotropin and thyroid hormone concentrations were determined by specific radioimmunoassay at the end of lactation. Animals receiving high-fat and low-protein diet had a significantly lower body weight (13.9% at weaning, P < 0.05) and serum albumin (25%, P < 0.05) and thyrotropin (26.2%, P < 0.01) concentrations, and a higher serum triiodothyronine concentration (74%, P < 0.005) and 131I-thyroid uptake (77%, P < 0.005). These data show that a high-fat and low-protein diet can promote maternal thyroid hyperfunction that differs from the thyroid hypofunction observed in dams fed a low-protein diet, a phenomenon that can be of adaptive importance for pup nurturing.
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Animales , Femenino , Masculino , Embarazo , Ratas , Adaptación Fisiológica/fisiología , Dieta con Restricción de Proteínas , Grasas de la Dieta/metabolismo , Lactancia/metabolismo , Glándula Tiroides/fisiología , Hormonas Tiroideas/análisis , Peso Corporal , Ingestión de Energía , Radioinmunoensayo , Ratas Wistar , Albúmina Sérica/análisis , Pruebas de Función de la Tiroides , Glándula Tiroides/metabolismo , Hormonas Tiroideas/metabolismoRESUMEN
BACKGROUND: The Brazilian population has been the focus of intensive genetic study due to admixture characteristics whereas there are few reports on the variability of VNTR loci in Brazil. PRIMARY OBJECTIVE: The aim of this study was to analyse genetic parameters in sample populations from two geographically distant regions: São Luis City, in Maranhão State and Campinas City, in São Paulo State. We investigated if distinct colonization influences could produce detectable differences in genetic background. SUBJECT AND METHODS: DNA samples from peripheral drained blood were obtained from unrelated individuals who underwent paternity testing. Allelic variation in six VNTR loci (D2S44, D4S139, D5S110, D8S358, DI0S28 and D17S79) was evaluated. The results were compared to reference databases available for general Latin-derived European and African-American populations as well as for other Brazilian groups. RESULTS: This study reveals that forensic population parameters did not show differences among regions, although we detected admixture values varying between the south-east and north-east of Brazil. CONCLUSIONS: Differences between the two samples are probably due to different admixture proportions of European- and African-derived alleles in each region: both populations are in Hardy Weinberg equilibrium. In addition, the allelic frequency for all loci, in both populations, can be used as database for forensic purposes.
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Variación Genética , Repeticiones de Minisatélite , Alelos , Población Negra/genética , Southern Blotting , Brasil , Frecuencia de los Genes , Genética de Población , Humanos , Población Blanca/genéticaRESUMEN
BACKGROUND: Necrobiotic xanthogranuloma (NXG) is a rare non-X histiocytosis with conspicuous lesions on the periorbital skin. METHODS: A diabetic patient presented with NXG and a previous diagnosis of necrobiosis lipoidica on the legs over a period of almost 2 years before the development of the typical lesions of NXG on the periorbital regions, back, thighs and legs. The patient was found also to have developed lambda paraproteinemia. RESULTS: Treatment with melphalan and prednisone resulted in great improvement of cutaneous lesions and paraproteinemia remission. CONCLUSION: This case report details how melphalan and prednisone can be administered in the successful treatment of necrobiotic xanthogranuloma with lambda paraproteinemia.
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Antiinflamatorios/uso terapéutico , Histiocitosis/tratamiento farmacológico , Melfalán/uso terapéutico , Necrobiosis Lipoidea/tratamiento farmacológico , Paraproteinemias/tratamiento farmacológico , Prednisona/uso terapéutico , Adulto , Diagnóstico Diferencial , Quimioterapia Combinada , Femenino , Histiocitosis/complicaciones , Histiocitosis/diagnóstico , Humanos , Necrobiosis Lipoidea/complicaciones , Necrobiosis Lipoidea/diagnóstico , Paraproteinemias/complicaciones , Paraproteinemias/diagnósticoRESUMEN
The prevalence of antithrombin (AT) deficiency in 342 unselected Brazilian patients with venous thrombosis was 1.16%, which increased to 3% when only patients under the age of 50 or with a familial history of thrombosis were considered. In two patients, a clinical (contraceptive use) or genetic risk factor (factor V Leiden and C677T in the methylene tetrahydrofolate reductase gene [MTHFR]) was identified and corroborated the hypothesis that an interaction of factors accounted for the appearance of thrombosis. However, no risk factor other than AT deficiency was identified in one patient with an important clinical and family history of spontaneous thrombosis. Three mutations were identified in these patients: a G-->A transition in intron 5 at position +1 (5'-->3'), three base insertions corresponding to arginine at position 5383 in exon 3A, and a G-->A transition at 13328, corresponding to an Ala404Thr de novo mutation. The polymorphisms in the genes coding for coagulation factors XII and XIII and fibrinogen normally associated with an increased risk for venous thrombosis were not related to thrombosis in these patients. This is the first study in South America to assess the prevalence of AT deficiency and to report the molecular characterization of the mutations involved.
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Deficiencia de Antitrombina III/genética , Mutación/genética , Trombosis de la Vena/genética , Adulto , Anciano , Antitrombina III/genética , Brasil/epidemiología , Análisis Mutacional de ADN , Femenino , Humanos , Masculino , Persona de Mediana Edad , Linaje , Mutación Puntual , Prevalencia , Sitios de Empalme de ARN , Factores de Riesgo , Trombosis de la Vena/epidemiología , Trombosis de la Vena/etiologíaRESUMEN
Lactating rats were fed with free access to an 8% protein-restricted diet (PR); the control group was fed a 23% protein diet (C). An energy-restricted (pair-fed) group was given the same food as the animals in the control group, but the amounts of food consumed by both PF and PR were about the same. The body weight and serum albumin concentration of PR and PF dams were significantly (P < 0. 05) lower than that of the controls. The PR group had a significant increase in serum-free triiodothyronine (FT3) concentration, 24-hr mammary gland and milk radioiodine (I131) uptake (67%, 278%, and 200%, respectively) as compared with the controls. On the other hand, those animals had a significantly lower serum-free thyroxine (FT4) concentration and 2- and 24-hr thyroid I131 uptake (67%, 64%, and 74%, respectively). Protein malnutrition during lactation did not alter thyroid or liver 5'-deiodinase activity significantly. However, PF dams had a significantly lower (25%) thyroid 5'-deiodinase activity. These data suggest that protein-restricted lactating dams had an adaptive change in the thyroid function, which could be important to increase the transference of iodine or triiodothyronine through the milk to their pups and prevent sequelae of neonatal hypothyroidism.
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Dieta con Restricción de Proteínas/efectos adversos , Lactancia/fisiología , Glándula Tiroides/metabolismo , Animales , Peso Corporal , Femenino , Hipotiroidismo/fisiopatología , Yoduro Peroxidasa/metabolismo , Radioisótopos de Yodo , Hígado/enzimología , Hígado/metabolismo , Glándulas Mamarias Animales/metabolismo , Leche/química , Leche/metabolismo , Trastornos Nutricionales/fisiopatología , Tamaño de los Órganos , Ratas , Ratas Wistar , Albúmina Sérica/análisis , Glándula Tiroides/enzimología , Tirotropina/sangre , Tiroxina/sangre , Triyodotironina/sangreRESUMEN
The effect of malnutrition on the biodistribution of radiopharmaceuticals is not known. We studied the biodistribution of 99Tcm-labelled sodium pertechnetate (Na99TcmO4) in two rat models of malnutrition. Three groups of 2-month-old rats were separated according to their diets: (1) control diet, 23% protein (C); (2) protein-restricted, receiving 8% protein (PR), both ad libitum; and (3) energy-restricted, receiving 60% of control diet (ER). After 21 days of the diet, 99Tcm was injected and the animals were killed after 30 min. The organs were isolated, their weight determined and the absolute per cent (%ID) and the per cent per gram injected dose (%ID x g(-1)) calculated. The %ID and %ID x g(-1) had a similar pattern, increasing in stomach and brain and decreasing in the thyroid, but did not change significantly in kidney, lung, liver, bone or testis in PR rats, except in the heart where the increase was only observed in the %ID x g(-1). In the ER group the %ID x g(-1) was decreased in the bone only, and did not change in the other organs. It is suggested that when using Na99TcmO4 scintigraphy in malnourished patients, the different patterns of distribution must be kept in mind.
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Desnutrición Proteico-Calórica/metabolismo , Radiofármacos/farmacocinética , Pertecnetato de Sodio Tc 99m/farmacocinética , Animales , Dieta , Carbohidratos de la Dieta/farmacología , Grasas de la Dieta/farmacología , Proteínas en la Dieta/farmacología , Masculino , Ratas , Ratas Wistar , Distribución TisularRESUMEN
This study was designed to evaluate the thyroid and pituitary hormone levels in post-weaning rats whose dams were fed a low-protein diet during suckling (21 days). The dams and pups were divided into 2 groups: a control group fed a diet containing 22% protein that supplies the necessary amount of protein for the rat and is the usual content of protein in most commercial rat chow, and a diet group fed with a low-protein (8%) diet in which the protein was substituted by an isocaloric amount of starch. After weaning all dams and pups received the 22% protein diet. Two hours before sacrifice of pups aged 21, 30 and 60 days, a tracer dose (0.6 microCi) of 125I was injected (i.p.) into each animal. Blood and thyroid glands of pups were collected for the determination of serum T4, T3 and TSH and radioiodine uptake. Low protein diet caused a slight decrease in radioiodine uptake at 21 days, and a significant decrease in T3 levels (128 +/- 14 vs 74 +/- 9 ng/dl, P < 0.05), while T4 levels did not change and TSH was increased slightly. At 30 days, T3 and TSH did not change while there was a significant increase in both T4 levels (4.8 +/- 0.3 vs 6.1 +/- 0.2 micrograms/dl, P < 0.05) and in radioiodine uptake levels (0.34 +/- 0.02 vs 0.50 +/- 0.03%/mg thyroid, P < 0.05). At 60 days serum T3, T4 and TSH levels were normal, but radioiodine uptake was still significantly increased (0.33 +/- 0.02 vs 0.41 +/- 0.03%/mg thyroid, P < 0.05). Thus, it seems that protein malnutrition of the dams during suckling causes hypothyroidism in the pups at 21 days that has a compensatory mechanism increasing thyroid function after refeeding with a 22% protein diet. The radioiodine uptake still remained altered at 60 days, when all the hormonal serum levels returned to the normal values, suggesting a permanent change in the thyroid function.
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Animales Lactantes/crecimiento & desarrollo , Dieta con Restricción de Proteínas , Glándula Tiroides/fisiología , Hormonas Tiroideas/biosíntesis , Tirotropina/biosíntesis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Animales Lactantes/fisiología , Femenino , Ratas , DesteteRESUMEN
This study was designed to evaluate the thyroid and pituitary hormone levels in post-weaning rats whose dams were fed a low-protein diet during suckling (21 days). The dams and pups were divided into 2 groups:a control group fed a diet containing 22 per cent protein that supplies the necessary amount of protein for the rat and is the usual content of protein in most commercial rat chow, and a diet group fed a lowprotein (8 per cent) diet in which the protein was substituted by an isocaloric amount of starch. After weaning all dams and pups received the 22 per cent protein diet. Two hours before sacrifice of pups aged 21, 30 and 60 days, a tracer dose (0.6 mui) ofl25I was injected (ip) into each animal. Blood and thyroid glands of pups were collected for the determination of serum T4, T3 and TSH and radioiodine uptake. Low protein diet caused a slight decrease in radioiodine uptake at 21 days, and a significant decrease in T3 levels (l28 ñ 14 vs 74 ñ 9 ng/dl, P<0.05), while T4 levels did not change and TSH was increased slightly. At 30 days, T3 and TSH did not change while there was a significant increase in both T4 levels (4.8 ñ 0.3 vs 6.1 ñ 0.2 mug/dl, P<0.05) and in radioiodine uptake levels (0.34 ñ 0.02 vs 0.50 ñ 0.030 per cent/mg thyroid, P<0.05). At 60 days serum T3, T4 and TSH levels were normal, but radioiodine uptake was still significantly increased (0.33 ñ 0.02 vs 0.41 ñ 0.03 per cent/mg thyroid, P<0.05). Thus, it seems that protein malnutrition of the dams during suckling causes hypothyroidism in the pups at 21 days that has a compensatory mechanism increasing thyroid function after refeeding with a 22 per cent protein diet. The radioiodine uptake still remained altered at 60 days, when all the hormonal serum levels returned to the normal values, suggesting a permanent change in the thyroid function.
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Ratas , Animales , Femenino , Animales Lactantes/crecimiento & desarrollo , Dieta con Restricción de Proteínas , Glándula Tiroides/fisiología , Hormonas Tiroideas/biosíntesis , Tirotropina/biosíntesis , Destete , Fenómenos Fisiológicos Nutricionales de los Animales , Animales Lactantes/fisiologíaRESUMEN
We report on 2 cases of Y; autosome translocations. One is a male with normal external genitalia and 45,X karyotype without evidence of mosaicism or apparent translocation on cytogenetic analysis. In situ hybridization showed that the euchromatic portion of the Y-chromosome is translocated to the chromosome 15. The other case is a clinically trisomy 18 male patient, with modal number of 46, a small metacentric marker with appearance of an i(18p) and cytogenetic and molecular evidence of Y;18 translocation. The occurrence of Y;18 translocation associated with clinical signs of trisomy 18 is reported here for the first time.
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Cromosomas Humanos Par 15 , Cromosomas Humanos Par 18 , Translocación Genética , Trisomía , Cromosoma Y , Secuencia de Bases , Células Cultivadas , Niño , Bandeo Cromosómico , Cartilla de ADN , Humanos , Hibridación in Situ , Lactante , Recién Nacido , Cariotipificación , Masculino , Datos de Secuencia MolecularRESUMEN
1. The function of a Y human chromosomal DNA sequence was evaluated. The Y-5 probe was isolated from a flow-sorted chromosome library and detects Y-specific sequences. 2. The Y-5 probe and other Y-specific probes were used to analyze an XX male patient without ambiguous genitalia. 3. DNA sequences from the short arm of the chromosome Y that were detected with pDP1007 and pDP105 in the patient's genome explain the testis differentiation observed in this case. 4. Failure of the patient's DNA to hybridize to the Y-5 probe shows that the primitive gonads can differentiate into testes even in the absence of this chromosome region. In contrast, a gene controlling spermatogenesis may exist in this region because the patient is azoospermic.
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Sondas de ADN , Aberraciones Cromosómicas Sexuales , Diferenciación Sexual , Cromosoma Y/fisiología , Adolescente , Southern Blotting , Humanos , Cariotipificación , Masculino , Hibridación de Ácido NucleicoRESUMEN
The function of a Y human chromosomal DNA sequence was evaluated. The Y-5 probe was isolated from a flow-sorted chromosome library and detects Y-specific sequences. The Y-5 probe and other T-specific probes were used to analyze an XX male patient without ambiguous genitalia. DNA sequences from the short arm of the chromosome Y that were detected with pDP1007 and DP105 in the patient's genome explain the testis differentation observed in this case. Failure of the patient's DNA to hybridize to the Y-5 probe shows that the primitive gonads can differentiate into testes even in the absence of this chromosome region. In contrast, a gene controlling spermatogenesis may exist in this region because the patient azoospermic
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Humanos , Masculino , Adolescente , Sondas de ADN , Aberraciones Cromosómicas Sexuales , Análisis para Determinación del Sexo , Cromosoma Y/fisiología , Southern Blotting , Cariotipificación , Hibridación de Ácido NucleicoRESUMEN
Thyroid peroxidase (TPO) iodination activity is generally evaluated in vitro by the iodination of poorly iodinated thyroglobulin or bovine serum albumin, followed by separation of protein-bound and inorganic iodide by paper chromatography. Precipitation of protein-bound iodine by trichloroacetic acid (TCA) was evaluated as an alternative to the time-consuming paper chromatographic separation (PC) in normal rat TPO preparations. The protein-bound iodine estimates as well as the iodination activities determined by these two procedures were significantly correlated (r = 0.95 and 0.98, respectively, P less than 0.001), and the iodination activities regression line slope (b = 0.97 +/- 0.11) was not different from 1. The protein-bound iodine separation by TCA is simpler and faster, without loss of precision. Thus, it can be a useful alternative step in the thyroid peroxidase iodination assay.
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Yoduro Peroxidasa/metabolismo , Yodo/metabolismo , Glándula Tiroides/fisiología , Animales , Cromatografía en Papel , Técnicas In Vitro , Unión Proteica , Ratas , Ratas Endogámicas , Análisis de Regresión , Ácido Tricloroacético/farmacologíaRESUMEN
Thyroid peroxidase (TPO) iodination activity is generally evaluated in vitro by the iodination of poorly iodinated thyroglobulin or bovine serum albumin, followed by separation of protein-bound and inorganic iodide by paper chromatography,. Precipitation of protein-bound iodine by trichloroacetic acid (TCA) was evaluated as an alternative to the time-consuming paper chromatographic separation (PC) in normal rat TPO preparations. The protein-bound iodine estimates as well as the iodination activities determined by these two procedures were significantly correlated (r = 0.95 and 0.98, respectively, P<0.001), and the iodination activities regression line slope (b = 0.97 + or - 0.11) was not different from 1. The protein-bound iodine separation by TCA is simpler and faster, without loss of precision. Thus, it can be a useful alternative step in the thyroid peroxidase iodination assay
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Ratas , Animales , Glándula Tiroides/fisiología , Técnicas In Vitro , Yoduro Peroxidasa/metabolismo , Yodo/metabolismo , Ácido Tricloroacético/farmacología , Cromatografía en Papel , Unión Proteica , Ratas Endogámicas , Análisis de RegresiónRESUMEN
Serum thyroxine and triiodothyronine, radioiodide thyroid uptake and thyroid peroxidase (TPO) activity were studied over a 2 to 5 day period in fasting rats treated (F+) or not (F-) with TSH. In F- rats, TPO activity was transiently decreased on the 3rd day, whereas in F+ it was always higher than in controls. On the 5th day, the 2 h thyroid uptake of 131I decreased in F-, while the 24 h uptake increased in both F- and F+. Serum T3 and T4 decreased in both fasting groups. Thus, not all effects of fasting on rat thyroid function are reverted by TSH administration, suggesting intrinsic impairment of glandular function.