RESUMEN
BACKGROUND: Cortisone is derived from cortisol through the action of the enzyme 11ß-hydroxysteroid dehydrogenase type II, and it has gained importance in recent years as a biomarker of stress. This study aimed to develop and validate an assay for the measurement of cortisone in pig saliva and evaluate whether its concentration varies in stressful situations. For this purpose, a specific immunoassay was developed and validated analytically, and a study was performed to evaluate whether cortisone concentrations in saliva can vary under heat stress conditions. RESULTS: The assay proved to be accurate, reliable, and sensitive for the measurement of cortisone in pig saliva. The limit of detection of the assay was set at 0.006 ng/ml, and the lower limit of quantification was 0.023 ng/ml. It also correlated significantly with the results obtained by LCâMS/MS (P = 0.003; r = 0.64). In addition, the cortisone concentration in animals subjected to prolonged heat stress decreased significantly 15 days after treatment (P < 0.0001). CONCLUSIONS: According to these results, cortisone measured by this assay could be used as a tool for the non-invasive evaluation of thermal stress in pig saliva.
Asunto(s)
Cortisona , Saliva , Animales , Cortisona/análisis , Cortisona/metabolismo , Saliva/química , Porcinos , Estrés Fisiológico , Calor , Inmunoensayo/veterinaria , Reproducibilidad de los Resultados , Masculino , Espectrometría de Masas en Tándem/veterinaria , Femenino , Biomarcadores/análisisRESUMEN
Introducción: la musicoterapia se entiende por el uso de la música o sus elementos con fines terapéuticos. Su empleo en la enfermería es escaso más allá del uso de la intervención NIC 4400. Objetivo: conocer y analizar el grado de aceptación por parte de los estudiantes de enfermería ante la posible inclusión de la musicoterapia como asignatura en los programas educativos de los estudios de Grado en Enfermería. Método: se ha realizado un estudio observacional descriptivo de corte transversal con una muestra n=346 participantes. Resultados: los resultados muestran que el 93,93% de los alumnos sí incluirían la musicoterapia como una asignatura Optativa en los estudios de Grado en Enfermería, haciendo uso de ella en su futuro profesional
Introduction: music therapy is understood by the use of music or its elements for therapeutic purposes. Its use in nursing is scarce beyond the use of the NIC 4400 intervention. Objective: the research aims to know and analyze the degree of acceptance by nursing students regarding the possible inclusion of music therapy as a subject in the educational programs of the Nursing Degree studies. Method: a cross-sectional, observational, descriptive study was carried out with a sample of n=346 participants. Conclusion: the results show that 93.93% of the students did include music therapy as an optional subject in Nursing Degree studies, making use of it in their professional future