RESUMEN
We report herein the design, synthesis, and transfection biology of a novel series of non-glycerol-based cationic lipids with lactic acid-derived head groups The synthetic procedure adopted herein for preparing 1-hydroxy-prop-2-yl head-group-based monocationic transfection lipids 1-7 is fairly straightforward and potentially applicable in designing other cationic lipids with lactic acid-derived head groups. A striking anchor-length dependency was observed in NIH3T3 cells in the sense that except lipid 4, all the other lipids were essentially transfection-inefficient. Ethidium bromide assay for the lipid:DNA interactions is consistent with the general observation that significant lipid:DNA interactions do not guarantee on improved transfection efficiency cationic lipid mediated gene delivery. Given its remarkable transfection properties and low cellular toxicity, lipid 4 is likely to find future use in the area of liposomal gene delivery.
Asunto(s)
Lípidos/química , Lípidos/síntesis química , Células 3T3 , Animales , Células COS , ADN/administración & dosificación , ADN/química , Sistemas de Liberación de Medicamentos , Etidio , Colorantes Fluorescentes , Terapia Genética , Glicerol/química , Ácido Láctico/química , Lípidos/toxicidad , Liposomas , Espectroscopía de Resonancia Magnética , Ratones , Estructura Molecular , Espectrometría de Fluorescencia , TransfecciónRESUMEN
Injury to the inferior alveolar and lingual nerves may occur during removal of third molar teeth. An understanding of the possible mechanisms leading to nerve injury should reduce this occurrence. Careful assessment and documentation should follow a nerve injury, and early specialist referral considered. Although spontaneous recovery of low-grade nerve injuries is common, microneurosurgical techniques offer hope for more serious injuries.