RESUMEN
Rehabilitation of aged drinking water pipes is an extensive renovation and increasingly topical in many European cities. Spray-on-lining of drinking water pipes is an alternative cost-effective rehabilitation technology in which the insides of pipes are relined with organic polymer. A commonly used polymer is epoxy resin consisting of monomer bisphenol A (BPA). Leaching of BPA from epoxy lining to drinking water has been a concern among public and authorities. Currently epoxy lining is not recommended in some countries. BPA leaching has been demonstrated in laboratory studies but the behavior and ageing process of epoxy lining in situ is not well known. In this study 6 locations with different age epoxy linings of drinking water pipes done using two distinct technologies were studied. While bisphenol F, 4-n-nonylphenol, and 4-t-octylphenol were rarely found and in trace concentrations, BPA was detected in majority of samples. Pipes lined with the older technology (LSE) leached more BPA than those with more recent technology (DonPro): maxima in cold water were 0.25 µg/L and 10 ng/L, respectively. Incubation of water in pipes 8-10 h prior to sampling increased BPA concentration in cold water 1.1-43-fold. Hot water temperature caused even more BPA leaching - at maximum 23.5 µg/L. The influence of ageing of epoxy lining on BPA leaching on could be shown in case of LSE technology: locations with 8-9 years old lining leached 4-20-fold more BPA compared to a location with 2-year-old lining. Analysis of metals showed that epoxy lining can reduce especially iron concentration in water. No significant burden to water could be shown by the analyzed 72 volatile organic compounds, including epichlorhydrin, precursor used in epoxy resin. Estrogenicity was detected in water samples with the highest BPA loads. Comparable responses of two yeast bioreporters (estrogen receptor α and BPA-targeted) indicated that bisphenol-like compounds were the main cause of estrogenicity. Compared to the estimated average daily BPA exposure, additional BPA load via cold drinking water in the studied locations was low, maximum 8.7%. However, hot water should also be considered as exposure source due to higher BPA concentrations. Epoxy lined locations should be monitored in future in order to evaluate ageing process and control increasing leaching of potentially harmful chemicals.
Asunto(s)
Agua Potable/química , Resinas Epoxi , Compuestos de Bencidrilo , Ciudades , Humanos , Fenoles/química , Contaminantes Químicos del Agua , Contaminación del AguaRESUMEN
The continuously increasing genome sequencing data has revealed numerous cryptic pathways, which might encode novel secondary metabolites with interesting biological activities. However, utilization of this hidden potential has been hindered by the observation that many of these gene clusters remain silent (or poorly expressed) under laboratory conditions. Here we present reporter-guided mutant selection (RGMS) as an effective and widely applicable method for targeted activation of silent gene clusters in the native producers. The strategy takes advantage of genome-scale random mutagenesis for generation of genetic diversity and a reporter-guided selection system for the identification of the desired target-activated mutants. It was first validated in the re-activation of jadomycin biosynthesis in Streptomyces venezuelae ISP5230, where high efficiency of activation was achieved. The same strategy was then applied to a hitherto unactivable pga gene cluster in Streptomyces sp. PGA64 leading to the identification of two new anthraquinone aminoglycosides, gaudimycin D and E.
Asunto(s)
Genes Bacterianos , Genes Reporteros , Familia de Multigenes , Mutación , Streptomyces , Antraquinonas/metabolismo , Isoquinolinas/metabolismo , Streptomyces/genética , Streptomyces/metabolismoRESUMEN
Bioluminescent Saccharomyces cerevisiae yeast-based bioreporters were used to monitor bisphenol A and other estrogenic chemicals in thermal paper samples collected mainly from Finland on two occasions in 2010/2011, and 2013. The bisphenol A-targeted (BPA-R) and the human oestrogen receptor (hERα) bioreporters were applied to analyse both non-treated and extracted paper samples. Bisphenol A was readily bioavailable to the yeast bioreporters on the non-treated paper samples without any pre-treatment. Detected concentrations ranged from a detection limit of 9-142 µg/g to over 20 mg/g of bisphenol A equivalents in the thermal papers. Low bisphenol A like activities were detected in many samples, and were considered to be caused by residual bisphenol A or other types of bisphenols, such as bisphenol S. Most of the thermal paper samples were toxic to the yeast bioreporters. The toxicity did not, however, depend on the bisphenol A concentration of the samples. The yeast bioreporters were demonstrated to be a robust and cost-efficient method to monitor thermal paper samples for their bisphenol A content and estrogenicity. Thermal paper was considered as a potential BPA source for both human exposure and environmental emission.
Asunto(s)
Compuestos de Bencidrilo/análisis , Técnicas Biosensibles/métodos , Receptor alfa de Estrógeno/química , Fenoles/análisis , Saccharomyces cerevisiae/metabolismo , Compuestos de Bencidrilo/metabolismo , Técnicas Biosensibles/instrumentación , Receptor alfa de Estrógeno/genética , Receptor alfa de Estrógeno/metabolismo , Humanos , Límite de Detección , Papel , Fenoles/metabolismo , Saccharomyces cerevisiae/químicaRESUMEN
The first Saccharomyces cerevisiae yeast bioreporter for analysis of a single endocrine disrupting compound, bisphenol A (BPA), was developed. The bioreporter contains mutated human estrogen receptor α (hERα), called bisphenol A-targeted receptor (BPA-R). The BPA-R bioreporter was characterized with mixtures of estrogenic chemicals and tested with spiked influent wastewater samples. The detection limit for BPA was 4.2-fold lower (0.107 µM, i.e., 24 µg L(-1)), while that of the native hormone 17ß-estradiol (E2) (1 µM, i.e., 272 µg L(-1)) was 166,000-fold higher compared to the wild type hERα bioreporter. The BPA-R bioreporter responded only to BPA in a chemical cocktail and spiked concentrated wastewater samples with high concentrations of other estrogenic chemicals. As a conclusion, wastewater and other environmental water samples can be concentrated and specifically analyzed for BPA without risk of the mixture effect caused by other estrogenic chemicals. The BPA-R bioreporter is a robust and cost-efficient choice for high-throughput monitoring of BPA and its bioavailability in complex samples.
Asunto(s)
Compuestos de Bencidrilo/química , Receptor alfa de Estrógeno/análisis , Fenoles/química , Saccharomyces cerevisiae/química , Humanos , Límite de DetecciónRESUMEN
Organotin compounds are toxic and endocrine disrupting compounds, which have been intensively used as antifouling paints for ship hulls and thus are widely spread in the environment. They are suspected to cause imposex, the formation of male characteristics in female gastropods, because of the activation of retinoid X receptor (RXR) at very low environmental concentrations. Here we report the development and optimization of a bioluminescent yeast assay for the detection of organotin compounds based on the interaction with a hybrid RXR and subsequent expression of a reporter luciferase gene. This assay is highly specific toward organotin compounds and natural ligands of the RXR. It detects tributyltin and triphenyltin in nanomolar concentrations (detection limits were found to be 30 nM and 110 nM, respectively) and allows small-scale high-throughput analyses. Furthermore it was possible to measure tributyltin directly in untreated spiked sediments. Thus, the results provided within one working day can be used for the assessment of bioavailability and mixture effect of organotin compounds in environmental samples.
Asunto(s)
Proteínas Fúngicas/análisis , Mediciones Luminiscentes/métodos , Compuestos Orgánicos de Estaño/análisis , Saccharomyces cerevisiae/química , Receptor alfa de Estrógeno/química , Receptor alfa de Estrógeno/metabolismo , Humanos , Unión Proteica/fisiologíaRESUMEN
The quality of sewage sludge-based products, such as composts and growth media, is affected by the contamination of sewage sludge with, potentially, hundreds of different substances. Therefore, it is difficult to achieve the reliable environmental quality assessment of sewage sludge-based products solely based on chemical analysis. In the present work, we demonstrate the use of the kinetic luminescent bacteria test (ISO 21338) to evaluate acute toxicity and the Vitotox™ test to monitor genotoxicity of sewage sludge and composted sewages sludge. In addition, endocrine-disrupting and dioxin-like activity was studied using yeast-cell-based assays. The relative contribution of industrial waste water treated at the Waste Water Treatment Plants led to elevated concentrations of polyaromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and polychlorinated dibenzo-p-dioxins and -furans (PCDD/F) in sewage sludge. The effect of elevated amounts of organic contaminants could also be identified with biotests able to demonstrate higher acute toxicity, genotoxicity, and potential for endocrine-disruptive properties. Additional extraction steps in kinetic luminescent bacteria test with DMSO and hexane increased the level of toxicity detected. Composting in a pilot-scale efficiently reduced the amounts of linear alkylbenzensulphonates (LASs), nonylphenols and nonylphenolethoxylates (NPE/NPs) and PAH with relative removal efficiencies of 84%, 61% and 56%. In addition, decrease in acute toxicity, genotoxicity and endocrorine-disrupting and dioxin-like activity during composting could be detected. However, the biotests did have limitations in accessing the ecotoxicity of test media rich with organic matter, such as sewage sludge and compost, and effects of sample characteristics on biotest organisms must be acknowledged. The compost matrix itself, however, which contained a high amount of nutrients, bark, and peat, reduced the sensitivity of the genotoxicity tests and yeast bioreporter assays.
Asunto(s)
Aguas del Alcantarillado , Suelo , Pruebas de Toxicidad/métodos , Contaminantes Químicos del Agua/toxicidad , Aliivibrio fischeri/efectos de los fármacos , Benzofuranos/análisis , Benzofuranos/toxicidad , Dibenzofuranos Policlorados , Dioxinas/análisis , Dioxinas/toxicidad , Ecotoxicología/métodos , Disruptores Endocrinos/análisis , Disruptores Endocrinos/toxicidad , Mediciones Luminiscentes , Pruebas de Mutagenicidad , Bifenilos Policlorados/análisis , Bifenilos Policlorados/toxicidad , Hidrocarburos Policíclicos Aromáticos/análisis , Hidrocarburos Policíclicos Aromáticos/toxicidad , Aguas del Alcantarillado/análisis , Aguas del Alcantarillado/química , Pruebas de Toxicidad Aguda/métodos , Contaminantes Químicos del Agua/análisis , Levaduras/efectos de los fármacosRESUMEN
Directed evolution has become a successful approach to alter ligand binding properties of nuclear receptors. In this study, directed evolution was used to generate a mutant human estrogen receptor α library, which was then used to screen for receptors having enhanced responses to the known endocrine-disrupting chemical, bisphenol A (BPA). A single round of multi-site mutation was combined with an efficient positive/negative library screening method in which positive growth-based selection for the desired activity with BPA was combined with flow cytometric removal of cells having undesired activity with the natural ligand, 17ß-estradiol. The screening steps were performed in a Saccharomyces cerevisiae yeast strain containing a genome-integrated his3-yEGFP reporter gene fusion construct. A single round of mutation and screening yielded nine mutants with enhanced responses towards BPA but no detectable induction by 17ß-estradiol (up to 90 nM). These BPA-specific mutant receptors may prove useful in the field of environmental analytics, where they could be used to monitor and evaluate the proportion of BPA in hormonally active samples.
Asunto(s)
Evolución Molecular Dirigida/métodos , Receptor alfa de Estrógeno/metabolismo , Fenoles/metabolismo , Compuestos de Bencidrilo , Receptor alfa de Estrógeno/genética , Humanos , Tamizaje Masivo/métodos , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , Unión Proteica , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismoRESUMEN
Bisphenol A is an endocrine disrupting compound, which is ubiquitous in the environment due to its wide use in plastic and resin production. Seven day old cultures of the litter-decomposing fungus Stropharia coronilla removed the estrogenic activity of bisphenol A (BPA) rapidly and enduringly. Treatment of BPA with purified neutral manganese peroxidase (MnP) from this fungus also resulted in 100% reduction of estrogenic activity, as analyzed using a bioluminescent yeast assay, and in the formation of polymeric compounds. In cultures of Stropharia rugosoannulata, estrogenic activity also quickly disappeared but temporarily re-emerged in the further course of cultivation. LC-MS analysis of the extracted estrogenic culture liquid revealed [M-H](-) ions with m/z values of 219 and 235. We hypothesize that these compounds are ring fission products of BPA, which still exhibit one intact hydroxyphenyl group to interact with estrogen receptors displayed by the yeast.
Asunto(s)
Agaricales/metabolismo , Disruptores Endocrinos/metabolismo , Fenoles/metabolismo , Contaminantes del Suelo/metabolismo , Agaricales/clasificación , Agaricales/enzimología , Compuestos de Bencidrilo , Biodegradación Ambiental , Disruptores Endocrinos/análisis , Disruptores Endocrinos/química , Peroxidasas/metabolismo , Fenoles/análisis , Fenoles/química , Contaminantes del Suelo/análisis , Contaminantes del Suelo/químicaRESUMEN
A panel of luminescent Saccharomyces cerevisiae cell-based nuclear receptor assays, consisting of human estrogen receptors α and ß, androgen receptor, and aryl hydrocarbon receptor, was miniaturized from the standard 96-well microplate format to high-throughput 384- and 1536-well microplate formats. In these assays, firefly luciferase lacking the peroxisome targeting sequence was used as a reporter and D-luciferin substrate was pre-mixed with the yeast cells before the incubation step, eliminating cell lysis and substrate addition steps, and allowing multiple readings at any desired time point. All of the assays were highly functional in the 384-well format, and most functioned well in the 1536-well format. The detection limit of the estrogen receptor α assay was even lower in the miniaturized microplate formats than in the original 96-well format. The panel of yeast-cell-based nuclear receptor assays can be used for high-throughput chemical testing and environmental monitoring of potential endocrine-disrupting activity of compounds and samples.