RESUMEN
The controlled release of drug from drug carrier has been a point of concern for the researchers to ensure the bioavailability of drug with reduced side effects. The formulation in this study is based upon biopolymers; carrageenan (CG), sodium alginate (SA) and various molecular weights of polyethylene glycol (PEG), cross-linked with (3-Aminopropyl)triethoxysilane, APTES for the sustained release of model drug (lidocaine). The physicochemical properties of the formulated hydrogel blends include bonding pattern (using Fourier Transform Infra-Red Spectroscopy (FTIR), Thermogravimetric analysis (TGA), X-ray diffraction (XRD), swelling study, antimicrobial activity and morphology of hydrogel films was analyzed by scanning electron microscopy (SEM). The as-prepared hydrogels show an improved cell compatibility against 3T3 cell line as well as cell proliferation and kinetics of drug release showed that these hydrogels are potential for controlled release of lidocaine, a numbing agent. GAP 60 exhibited maximum swelling percent (910%) and was employed to load the drug. By using in vitro model, the drug release was studied in PBS solution. Non-Fickian and other kinetic models (Zero order, Higuchi, Hixson, Korsmeyer Peppas and Baker-Lonsdale) for diffusion were followed in results. The improved properties showed that the formulated hydrogels can easily be used for the sustain drug release studies.
Asunto(s)
Alginatos/química , Carragenina/química , Liberación de Fármacos , Hidrogeles/química , Lidocaína/farmacología , Células 3T3 , Animales , Antiinfecciosos/farmacología , Tampones (Química) , Supervivencia Celular/efectos de los fármacos , Preparaciones de Acción Retardada/farmacología , Escherichia coli/efectos de los fármacos , Hidrogeles/síntesis química , Concentración de Iones de Hidrógeno , Iones , Cinética , Ratones , Pruebas de Sensibilidad Microbiana , Polietilenglicoles/química , Soluciones , Espectroscopía Infrarroja por Transformada de Fourier , Termogravimetría , Factores de Tiempo , Difracción de Rayos XRESUMEN
Chitosan (biopolymer) and polyvinyl pyrolidone (PVP) with aminopropyletriethoxy silane (cross linker) based hydrogels were prepared and tested for controlled drug release. The drug release and kinetics were studied as a function of pH. Formulations were characterized by Fourier Transform Infrared (FTIR) and Thermogravimetric (TGA) analysis and TAP 32 hydrogel formulation was the most stable and hydrogel samples showed promising antibacterial activity against E. coli strain. The maximum swelling (4386%) was observed for TAP 32 formulation in distilled water, which was decreased with the concentration of ions. The diffusion exponent (n) values of all hydrogel formulations were recorded to be <0.5, which is an indication of Quasi-Fickian diffusion. The maximum swelling was observed at pHâ¯2 and decreased at higher pH. The pH sensitivity of hydrogels found to be promising for their use in drug delivery, which was tested for cefixime drug. Drug release of 81.6% was observed for the period of 12â¯h in a simulated gastric fluid (SGF). The values of R2 for zero order, first order, Higuchi, Hixson, Korsmeyer-Peppas and Baker-Lonsdale were 0.97, 0.9818, 0.99, 0.99, 0.88 and 0.80, respectively. The hydrogels based on chitosan and PVP revealed potential for controlled cefixime drug release in gastric pH medium.