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An Acad Bras Cienc ; 92(1): e20190658, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32428089

RESUMEN

To investigated the role of HIF-1α in myocardial inflammatory injury in rats induced by CME and its possible mechanism. Forty SD rats were separated randomly and equally into four groups, i.e. CME+HIF-1α stabilizer dimethyloxalyl glycine (CME+DMOG) group, CME+HIF-1α inhibitor YC-1 (CME+YC-1) group, CME group, and Sham group. HBFP staining, myocardial enzyme assessment, and cardiac ultrasound were used to measure microinfarct, serum c-troponin I (cTnI) level, and Cardiac function. ELISA and western blot were applied for detecting NLRP3 inflammasome pathway and TLR4/MyD88/NF-κB signaling level.Pro-inflammatory factors of IL-18, IL-1ß and TNF-α increased their expression levels after CME, which indicated inflammatory responses in the myocardium. Additionally, in the inflammatory process, NLRP3 inflammasome and TLR4/MyD88/NF-κB signaling were involved. DMOG reverses these effects of CME, whereas YC-1 aggravates these effects. HIF-1α may attenuate myocardial inflammatory injury induced by CME and improve cardiac function, which can perhaps be explained by the fact that TLR4/MyD88/NF-κB signaling pathway activation is inhibited.


Asunto(s)
Enfermedad de la Arteria Coronaria/complicaciones , Circulación Coronaria/fisiología , Subunidad alfa del Factor 1 Inducible por Hipoxia/fisiología , Isquemia Miocárdica/complicaciones , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Animales , Enfermedad de la Arteria Coronaria/fisiopatología , Trombosis Coronaria , Modelos Animales de Enfermedad , Ensayo de Inmunoadsorción Enzimática , Inflamasomas/metabolismo , Inflamación , Infarto del Miocardio/complicaciones , Ratas , Ratas Sprague-Dawley , Troponina/sangre
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