RESUMEN

In this study, a laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) method for in-situ determination of yttrium and trace elements in yttrium-doped barium fluoride (BaF2: Y) crystals was proposed. A facile, micro-damage procedure for quantifying the segregation coefficient of doping elements was investigated, and it was found that the actual yttrium doping concentration increases from the seed end to the tail end in BaF2: Y crystals. In micro-area analysis, this method has higher mass sensitivity which was applied to quantify the impurity content and distribution during the growth of BaF2: Y crystals. Regression coefficient of calibration curve for each element ranged from 0.9918 to 0.9995. Detection limits (DLs) were 0.05, 0.03, 0.01 and 0.01 µg g-1 for Mg, Zn, Sr and Pb, respectively. The accuracy of the proposed method was verified by inductively coupled plasma mass spectrometry/atomic emission spectroscopy (ICP-MS/AES) with wet-chemical pretreatment. The objective of the presented work was to provide a less damaging and more novelty approach for crystal sample analysis.

Asunto(s)

Terapia por Láser , Oligoelementos , Oligoelementos/análisis , Itrio , Análisis Espectral , Espectrometría de Masas/métodos

RESUMEN

Mg-based stent is a promising candidate of the next generation fully degradable vascular stents. The latest progress includes the CE approval of the Magmaris ® WE43 based drug eluting stent. However, so far, the long term (more than 1 year implantation) in vivo degradation and the physiological effects caused by the degradation products were still unclear. In this study, a 20 month observation was carried out after the bare Mg-Nd-Zn-Zr (abbr. JDBM) stent prototype was implanted into the common carotid artery of New Zealand white rabbit in order to evaluate its safety, efficacy and especially degradation behavior. The degradation of the main second phase Mg12Nd was also studied. Results showed that the bare JDBM stent had good safety and efficacy with a complete re-endothelialization within 28 days. The JDBM stent struts were mostly replaced in situ by degradation products in 4 month. The important finding was that the volume and Ca concentration of the degradation products decreased in the long term, eliminating the clinicians' concern of possible vessel calcification. In addition, the alloying elements Mg and Zn in the stent could be safely metabolized as continuous enrichment in any of the main organs were not detected although Nd and Zr showed an abrupt increase in spleen and liver after 1 month implantation. Collectively, the long term in vivo results showed the rapid re-endothelialization of JDBM stent and the long term safety of the degradation products, indicating its great potential as the backbone of the fully degradable vascular stent. STATEMENT OF SIGNIFICANCE: Mg-based stent is a promising candidate of the next generation fully degradable stents, especially after the recent market launch of one of its kind (Magmaris). However the fundamental question about the long term degradation and metabolic mechanism of Mg-based stent and its degradation products remain unanswered. We implanted our patented Mg-Nd-Zn-Zr bare stent into the common carotid artery of rabbits and conducted a 20 months observation. We found that the Ca containing degradation products could be further degraded in vivo. All the alloying elements showed no continuous enrichment in the main organs of rabbits. These findings eliminate the clinicians' concern of possible vessel calcification and element enrichment after the implantation of Mg alloy based stents to some extent.

Asunto(s)

Implantes Absorbibles , Aleaciones , Arteria Carótida Común/cirugía , Ensayo de Materiales , Stents , Aleaciones/química , Aleaciones/farmacocinética , Aleaciones/farmacología , Animales , Transporte Biológico Activo , Magnesio/química , Magnesio/farmacocinética , Magnesio/farmacología , Neodimio/química , Neodimio/farmacocinética , Neodimio/farmacología , Conejos , Estroncio/química , Estroncio/farmacocinética , Estroncio/farmacología , Zinc/química , Zinc/farmacocinética , Zinc/farmacología

RESUMEN

Chronic osteomyelitis and infected bone defects are substantial challenges faced by orthopaedic surgeons. In this study, vancomycin was loaded into mesoporous bioactive glass (MBG) to form a local antibiotic delivery system and then a bone tissue-engineering scaffold combining MBG and poly-(L-lactic-co-glycolic acid) (PLGA) was prepared by freeze-drying fabrication. In vitro degradation and water contact angle analysis suggested that the MBG-incorporated PLGA scaffold exhibited controlled degradability, stabilizing the pH of the surrounding environment and improved the hydrophilicity. Moreover, the presence of MBG provides a well-interconnected pore structure, to which human bone marrow-derived mesenchymal stem cells can attach, spread and proliferate, promoting upregulation of the expression of osteogenic markers. Thus, MBG/PLGA scaffolds exhibit better cytocompatibility and osteoblastic difierentiation properties compared with pure PLGA scaffolds. Vancomycin-loaded scaffolds have been found to yield sustained release that lasts for more than eight weeks in vitro. We tested the antibacterial performance of vancomycin-loaded scaffolds against Staphylococcus aureus, the most common bacteria isolated from infected bone. In vitro experiments demonstrated that loading vancomycin onto the scaffold promoted antibacterial activity and inhibited biofilm formation without deleterious effect on cytocompatibility. In conclusion, the novel inorganic-organic composites are considered potential materials for the treatment of infected bone defects.

Asunto(s)

Antibacterianos , Regeneración Ósea/efectos de los fármacos , Cerámica , Osteogénesis/efectos de los fármacos , Osteomielitis/tratamiento farmacológico , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Infecciones Estafilocócicas/tratamiento farmacológico , Staphylococcus aureus/fisiología , Vancomicina , Antibacterianos/química , Antibacterianos/farmacocinética , Antibacterianos/farmacología , Biopelículas/efectos de los fármacos , Biopelículas/crecimiento & desarrollo , Cerámica/química , Cerámica/farmacocinética , Cerámica/farmacología , Preparaciones de Acción Retardada/química , Preparaciones de Acción Retardada/farmacocinética , Preparaciones de Acción Retardada/farmacología , Humanos , Osteomielitis/metabolismo , Osteomielitis/patología , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/farmacocinética , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/farmacología , Porosidad , Infecciones Estafilocócicas/metabolismo , Infecciones Estafilocócicas/patología , Vancomicina/química , Vancomicina/farmacocinética , Vancomicina/farmacología

RESUMEN

The clinical potentials of radiotherapy could not be achieved completely because of the inaccurate positioning and inherent radioresistance of tumours. In this study, a novel active-targeting upconversion theranostic agent (arginine-glycine-aspartic acid-labelled BaYbF5: 2% Er(3+) nanocube) was developed for the first time to address these clinical demands. Heavy metal-based nanocubes (~10 nm) are potential theranostic agents with bifunctional features: computed tomography (CT) contrast agents for targeted tumour imaging and irradiation dose enhancers in tumours during radiotherapy. Remarkably, they showed low toxicity and excellent performance in active-targeting CT imaging and CT imaging-guided radiosensitizing therapy, which could greatly concentrate and enlarge the irradiation dose deposition in tumours to enhance therapeutic efficacy and minimize the damage to surrounding tissues.

Asunto(s)

Diagnóstico por Imagen/métodos , Metales Pesados/química , Nanoestructuras/química , Neoplasias/radioterapia , Fármacos Sensibilizantes a Radiaciones/química , Tomografía Computarizada por Rayos X/métodos , Línea Celular Tumoral , Medios de Contraste/química , Humanos , Radioterapia/métodos

RESUMEN

Nano-biotechnology has been introduced into cancer theranostics by engineering a new generation of highly versatile hybrid mesoporous composite nanocapsules (HMCNs) for manganese-based pH-responsive dynamic T(1)-weighted magnetic resonance imaging (MRI) to efficiently respond and detect the tumor acidic microenvironment, which was further integrated with ultrasonographic function based on the intrinsic unique hollow nanostructures of HMCNs for potentially in vitro and in vivo dual-modality cancer imaging. The manganese oxide-based multifunctionalization of hollow mesoporous silica nanoparticles was achieved by an in situ redox reaction using mesopores as the nanoreactors. Due to the dissolution nature of manganese oxide nanoparticles under weak acidic conditions, the relaxation rate r(1) of manganese-based mesoporous MRI-T(1) contrast agents (CAs) could reach 8.81 mM(-1)s(-1), which is a 11-fold magnitude increase compared to the neutral condition, and is almost two times higher than commercial Gd(III)-based complex agents. This is also the highest r(1) value ever reported for manganese oxide nanoparticles-based MRI-T(1) CAs. In addition, the hollow interiors and thin mesoporous silica shells endow HMCNs with the functions of CAs for efficient in vitro and in vivo ultrasonography under both harmonic- and B-modes. Importantly, the well-defined mesopores and large hollow interiors of HMCNs could encapsulate and deliver anticancer agents (doxorubicin) intracellularly to circumvent the multidrug resistance (MDR) of cancer cells and restore the anti-proliferative effect of drugs by nanoparticle-mediated endocytosis process, intracellular drug release and P-gp inhibition/ATP depletion in cancer cells.

Asunto(s)

Resistencia a Múltiples Medicamentos , Resistencia a Antineoplásicos , Imagen por Resonancia Magnética/métodos , Neoplasias/tratamiento farmacológico , Dióxido de Silicio/química , Ultrasonografía/métodos , Adsorción , Antineoplásicos/farmacología , Ciclo Celular , Línea Celular Tumoral , Medios de Contraste/farmacología , Colorantes Fluorescentes/química , Humanos , Concentración de Iones de Hidrógeno , Compuestos de Manganeso/química , Microscopía Confocal/métodos , Nanotecnología/métodos , Neoplasias/diagnóstico , Neoplasias/patología , Oxidación-Reducción , Óxidos/química , Polietilenglicoles/química

RESUMEN

To address the intractable issues such as the low performance or biocompatibility frequently encountered in previous CT, magnetic resonance (MR) and fluorescence trimodal imaging nanoprobes, a nanocomposite has been constructed by decorating gadolinium ions doped upconversion nanoparticle (Gd-doped UCNP) with radiopaque but fluorescence-transparent tantalum oxide (TaO(x), x ≈ 1). The as-synthesized water-soluble nanoparticle showed a litchi-like shape with an average size of ~30 nm and demonstrated extraordinarily high longitudinal and transverse relaxivity values (r(1) = 11.45 mM(-1)s(-1) and r(2) = 147.3 mM(-1)s(-1)) compared with the reported Gd-doped UCNPs to date. Obvious CT contrast enhancement was obtained by the combined effect between the radiopaque TaO(x) shell and the Gd-doped UCNP inner core. Strong upconversion luminescence (UCL) signal could unobstructedly penetrate out in virtue of high transparency of the TaO(x) shell. No mutual interference among different modalities of the upconversion nanolitchi (UCNL) was found, which ensured that the individual merits of every imaging modality could be brought into full play, demonstrated by in vitro and in vivo imagings. Furthermore, UCNLs showed only a slight effect on macrophages and RBCs in vitro and tissue in vivo.

Asunto(s)

Medios de Contraste/química , Imagen por Resonancia Magnética/métodos , Nanopartículas/química , Óxidos/química , Tantalio/química , Tomografía Computarizada por Rayos X/métodos , Animales , Muerte Celular , Línea Celular , Fluorescencia , Hemólisis , Humanos , Ratones , Microscopía Confocal , Nanopartículas/ultraestructura , Soluciones , Temperatura , Factores de Tiempo

RESUMEN

Early diagnosis that combines the high-resolutional CT and sensitive NIR-fluorescence bioimaging could provide more accurate information for cancerous tissues, which, however, remain a big challenge. Here we report a simple bimodal imaging platform based on PEGylated NaYbF(4): Tm(3+) nanoparticles (NPs) of less than 20 nm in diameter for both CT and NIR-fluorescence bioimaging. The as-designed nanoprobes showed excellent in vitro and in vivo performances in the dual-bioimaging, very low cytotoxicity and no detectable tissue damage in one month. Remarkably, the Yb(3+) in the lattice of NaYbF(4): Tm(3+) NPs functions not only as a promising CT contrast medium due to its high X-ray absorption coefficiency, but also an excellent sensitizer contributing to the strong NIR-fluorescent emissions for its large NIR absorption cross-section. In addition, these NPs could be easily excreted mainly via feces without detectable remnant in the animal bodies.

Asunto(s)

Nanopartículas , Espectrometría de Fluorescencia/métodos , Espectroscopía Infrarroja Corta/métodos , Tulio , Tomografía Computarizada por Rayos X/métodos , Iterbio , Itrio , Animales , Muerte Celular , Línea Celular , Medios de Contraste , Inyecciones Intravenosas , Ratones , Microscopía Confocal , Nanopartículas/ultraestructura , Especificidad de Órganos , Factores de Tiempo , Distribución Tisular , Imagen de Cuerpo Entero

RESUMEN

A modified SBA-15 mesoporous silica material NH(2)-SBA-15 was synthesized successfully by grafting γ-aminopropyl-triethoxysilane. The material was characterized using transmission electron microscopy (TEM) and Fourier transform infrared/Raman (FT-IR/Raman) spectroscopy, and used for the first time in a flow injection on-line solid phase extraction (SPE) coupled with flame atomic absorption spectrometry (FAAS) to detect trace Cr (VI). Effective sorption of Cr (VI) was achieved at pH 2.0 with no interference from Cr (III) and other ions and 0.5 mol L(-1) NH(3)·H(2)O solution was found optimal for the complete elution of Cr (VI). An enrichment factor of 44 and was achieved under optimized experimental conditions at a sample loading of 2.0 mL min(-1) sample loading (300 s) and an elution flow rate of 2.0 mL min(-1) (24s). The precision of the 11 replicate Cr (VI) measurements was 2.1% at the 100 µg L(-1) level with a detection limit of 0.2 µg L(-1) (3s, n=10) using the FAAS. The developed method was successfully applied to trace chromium determination in waste water. The accuracy was validated using a certified reference material of riverine water (GBW08607).

Asunto(s)

Cromo/análisis , Dióxido de Silicio/química , Extracción en Fase Sólida/métodos , Espectrofotometría Atómica/métodos , Contaminantes Químicos del Agua/análisis , Hidróxido de Amonio , Análisis de Inyección de Flujo , Concentración de Iones de Hidrógeno , Hidróxidos/química , Microscopía Electrónica de Transmisión , Silanos/química , Espectroscopía Infrarroja por Transformada de Fourier , Agua/química

RESUMEN

A microfluidic reactor has been developed for rapid enhancement of protein digestion by constructing an alumina network within a poly(ethylene terephthalate) (PET) microchannel. Trypsin is stably immobilized in a sol-gel network on the PET channel surface after pretreatment, which produces a protein-resistant interface to reduce memory effects, as characterized by X-ray fluorescence spectrometry and electroosmotic flow. The gel-derived network within a microchannel provides a large surface-to-volume ratio stationary phase for highly efficient proteolysis of proteins existing both at a low level and in complex extracts. The maximum reaction rate of the encapsulated trypsin reactor, measured by kinetic analysis, is much faster than in bulk solution. Due to the microscopic confinement effect, high levels of enzyme entrapment and the biocompatible microenvironment provided by the alumina gel network, the low-level proteins can be efficiently digested using such a microreactor within a very short residence time of a few seconds. The on-chip microreactor is further applied to the identification of a mixture of proteins extracted from normal mouse liver cytoplasm sample via integration with 2D-LC-ESI-MS/MS to show its potential application for large-scale protein identification.

Asunto(s)

Óxido de Aluminio , Proteínas/metabolismo , Animales , Cromatografía en Gel , Hidrólisis , Ratones , Polietilenglicoles/química , Tereftalatos Polietilenos , Espectrometría de Fluorescencia , Espectrometría de Masa por Ionización de Electrospray , Propiedades de Superficie , Espectrometría de Masas en Tándem

RESUMEN

A simple preparation scheme is described for the quantitative analysis of a magnesium niobate sample using slurry introduction axially viewed inductively coupled plasma optical emission spectrometry. Relationships between the stability of slurries and the conditions, such as particle size, pH, dispersant and amount of dispersant, were investigated experimentally. The MgNb(2)O(6) slurry sample was prepared by adding the dispersant sodium polyacrylate and agitation in an ultrasonic bath to ensure good dispersion. Under optimization of pH and amount of dispersant, an analysis of minor and trace impurities (Ba, Ca, Cr, Cu, Fe, Mn, Ni, Pb) in magnesium niobate was accomplished. Applying a paired t test, we showed that the results were in agreement at a 95% confidence level with the reference values obtained by a fusion method for a magnesium niobate sample, which verified that the calibration curves could be established by aqueous standards. Analytical results demonstrate that the factors that affected the accuracy of determination for MgNb(2)O(6) are mainly the particle size of the sample and the stability of slurry.

RESUMEN

An on-chip enzymatic reactor providing rapid protein digestion is presented. Trypsin-embedding stationary phase within the microchannel has been prepared by the sol-gel method. Such a microfluidic reactor has been used for low-level protein digestion at 16 fmol per analysis. The analytical potential of the microreactor combined with the strong cation exchange and RPLC ESI-MS/MS for the identification of real samples from the cytoplasma of the human liver tissue has been demonstrated.

Asunto(s)

Técnicas Analíticas Microfluídicas/métodos , Péptido Hidrolasas/metabolismo , Citoplasma/química , Humanos , Hígado/química , Técnicas Analíticas Microfluídicas/instrumentación , Gel de Sílice , Dióxido de Silicio , Espectrometría de Masa por Ionización de Electrospray , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Espectrometría de Masas en Tándem , Tripsina/metabolismo

RESUMEN

An effective method is presented for the on-chip analysis of chiral interactions with a successful depression of nonspecific adsorption. The alumina gel-derived protein network on poly(methyl methacrylate) (PMMA) microchannel was explored to form a protein-stationary phase and then used to carry out electrophoresis for fast enantioseparation coupled with electrochemical detection. On the basis of the chemical modification of a synthesized copolymer containing silane-functionalized scaffold, alumina sol-gel could react readily with the silane groups and form steady microstructure on the chip surface achieving the encapsulation of functional biomolecules. Compared with the native PMMA microchannels, the modified surfaces exhibited much better wettability, more stable and enhanced electroosmotic mobility, and less nonspecific adsorption. The water contact angle and EOF of alumina-gel-derived PMMA substrate were 22 degrees and 4.3 x 10(-4) cm(2) V(-1) s(-1), compared to those of 73 degrees and 1.9 x 10(-4) cm(2) V(-1) s(-1) from the untreated one, respectively. Bovine serum albumin, acting as a target protein, could be stably and homogeneously immobilized in the modified PMMA microchannel to fabricate a protein-stationary phase. Under a mild condition, D- and L-tryptophan were efficiently separated with a resolution of 1.57. The as-prepared microchip can perform chiral separations within short time, indicating that the general protocol has the potential to provide a platform for high throughput screening of enantiomer candidates such as those biochemical drugs with protein targets and the research of receptor interactions.

Asunto(s)

Análisis por Matrices de Proteínas/métodos , Proteínas/química , Adsorción , Sitio Alostérico , Óxido de Aluminio/química , Animales , Bovinos , Electroquímica , Microscopía Confocal , Microscopía Electrónica de Rastreo , Polímeros/química , Polimetil Metacrilato/química , Proteoma , Proteómica/métodos , Albúmina Sérica Bovina/química , Silanos/química , Factores de Tiempo , Triptófano/química

RESUMEN

Chemical modification of a poly(methyl methacrylate) (PMMA) microchannel surface has been explored to functionalize microfluidic chip systems. A craft copolymer was designed and synthesized to introduce the silane functional groups onto the plastic surface first. Furthermore, it has been found that, through a silicon-oxygen-silicon bridge that formed by tethering to these functional groups, a stable patterning network of gel matrix could be achieved. Thus, anchorage of proteins could be realized onto the hydrophobic PMMA microchannels with bioactivity preserved as far as possible. The protein homogeneous patterning in a microfluidic channel has been demonstrated by performing microchip capillary electrophoresis with laser-induced fluorescence detection and confocal fluorescence microscopy. To investigate the bioactivity of enzymes entrapped within stable silica gel-derived microchannels, the suggested scheme was employed to the construction of immobilized enzyme microreactor-on-a-chip. The proteolytic activity of immobilized trypsin has been demonstrated with the digestion of cytochrome c and bovine serum albumin at a fast flow rate of 4.0 microL/min, which affords the short residence time less than 5 s. The digestion products were characterized using MALDI-TOF MS with sequence coverage of 75 and 31% observed, respectively. This research exhibited a simple but effective strategy of plastic microchip surface modification for protein immobilization in biological and proteomic research.

Asunto(s)

Enzimas Inmovilizadas/química , Microfluídica/instrumentación , Proteínas/química , Secuencia de Aminoácidos , Reactores Biológicos , Datos de Secuencia Molecular , Polimetil Metacrilato

RESUMEN

Anthracycline drug daunomycin (DNR) is a widely used clinical drug. But its side effects, especially cardiotoxicity, have greatly restrained its application. The side effects were due to free radical formation in the metabolic process of DNR. The purpose of this study is to diminish the side effects by using antioxidants. Two kinds of free radical scavengers have been investigated, that is, vitamins: vitamin C (V(C)), rutin (V(P)) and vitamin B6 (V(B6)); amino acids: cysteine (CysH) and methionine (Met). Free radical scavenging efficiency (E degrees (eff)) of these antioxidants had been calculated. Under the experimental condition, the values of E degrees (eff) of V(C), V(P), V(B6), CysH and Met were 23.8, 15.3, 6.4, 48.2 and -7.7%, respectively. The relationship between the free radical scavenging activities and its chemical structure has also been discussed.

Asunto(s)

Antibióticos Antineoplásicos/química , Antioxidantes/química , Química Farmacéutica , Daño del ADN , Daunorrubicina/química , Interacciones Farmacológicas , Electroquímica