RESUMEN
Clinical examination with immune status assessment of more than 300 healthy persons, who worked in 30-km Chernobyl NPP zone four years after the accident was carried out. The clinical characters of immune deficiency were detected in 6.67% of examined persons and appeared as repeated respiratory diseases. Significant increase in mean level of leukocytes and lymphocytes was revealed. Activation of T-helper link of cell immunity was determined by increase of CD4+ cells in 35.2% of examined persons, that were lower than in people who took part in Chernobyl accident amelioration in 1986. Decrease in content of T-cells and B-cells was observed in 1/4 and 3/4 of persons correspondingly by rosette assays. No changes in serum immunoglobulins level was observed.
Asunto(s)
Síndromes de Inmunodeficiencia/etiología , Centrales Eléctricas , Liberación de Radiactividad Peligrosa , Adulto , Formación de Anticuerpos/efectos de la radiación , Relación CD4-CD8 , Femenino , Humanos , Inmunidad Celular/efectos de la radiación , Síndromes de Inmunodeficiencia/inmunología , Masculino , Persona de Mediana Edad , Factores de Tiempo , UcraniaRESUMEN
The presence of HIV provirus in the cell culture and in the patients' blood was studied by polymerase chain reaction followed by hybridization in solution. It was shown that: (i) the hybridized product could be detected both by gel electrophoresis and by binding on hydroxyapatite; (ii) the detection level achieved was no more than 10 infected lymphocytes per million; (iii) the hybridization signal and sensitivity of detection could be enhanced by the transcription of PCR product by the phage T7 RNA polymerase. The observed lack of complete correlation between the amount of provirus and of the p24 antigen in the patients' blood possibly reflects the peculiarities of HIV infection.
Asunto(s)
Pruebas Genéticas/métodos , Infecciones por VIH/genética , VIH-1/genética , Linfocitos/microbiología , Provirus/genética , Células Cultivadas , Cartilla de ADN , Sondas de ADN , ADN Viral/genética , Humanos , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico/métodos , Reacción en Cadena de la Polimerasa/métodosRESUMEN
The authors compared the responses of patients with atopic asthma to two cromolyn drugs of different generations, tilade and intal. As shown by clinical, allergological and immunological findings, tilade (sodium nedocromil) compared to intal is more active against atopic bronchial asthma complicated by obstructive bronchitis. It can more efficiently reduce specific and nonspecific bronchial hyperreactivity, is more potent against inflammation. Due to tilade, the need in glucocorticoid inhalation lowered, it became possible to induce a prolonged remission.
Asunto(s)
Asma/tratamiento farmacológico , Cromolin Sódico/uso terapéutico , Hipersensibilidad Inmediata/tratamiento farmacológico , Nedocromil/uso terapéutico , Adolescente , Adulto , Asma/complicaciones , Asma/diagnóstico , Bronquitis/tratamiento farmacológico , Bronquitis/etiología , Enfermedad Crónica , Evaluación de Medicamentos , Femenino , Humanos , Hipersensibilidad Inmediata/complicaciones , Hipersensibilidad Inmediata/diagnóstico , Masculino , Persona de Mediana EdadRESUMEN
Specific antibodies to persistent viruses (CMV, EBV, HBV) were detected by ELISA in groups of HIV-infected patients and persons showing indefinite results of the immunoblotting test for HIV-1 antigens, on the one hand, and in HIV-seronegative donors and patients with clinical manifestations of viral infection (CMVI) on the other. The findings indicate that the persons with indefinite immunoblotting test results show elevated blood CMV and HBV antigen levels than in the matched group of seronegative donors. This fact suggests that persistent viral infections might involve in the formation of an indefinite pattern when the sera were tested for HIV. The patients whose sera behaved in such a way represent a clinical risk group for HIV infection and call for further follow-up.