RESUMEN
BACKGROUND: The aim of this study was to investigate whether the perception of experimental pain was different during a mindfulness manipulation than during a distraction manipulation. Furthermore, it was examined if effects were moderated by dispositional pain catastrophizing. METHODS: Undergraduate students (n = 51) completed self-report measures of pain catastrophizing and mindfulness. Subsequently, they were administered a series of mildly painful heat stimuli, which they had to rate. During pain induction, participants listened to either a pre-recorded mindfulness instruction (mindfulness group) or a pre-recorded story (distraction group). RESULTS: After controlling for baseline experimental pain ratings, we found no overall group effect, indicating that there was no difference in experienced pain between the mindfulness group and the distraction group. However, a significant moderation effect was found. When dispositional pain catastrophizing was high, pain was less pronounced in the mindfulness group than in the distraction group, whereas the opposite effect was found when the level of pain catastrophizing was low. CONCLUSIONS: The findings suggest that in persons with a high level of catastrophic thinking about pain, mindfulness-based coping may be a better approach than distraction.
Asunto(s)
Atención/fisiología , Catastrofización/psicología , Individualidad , Atención Plena , Percepción del Dolor/fisiología , Adulto , Femenino , Humanos , Masculino , Adulto JovenRESUMEN
Data from the Genetic Association Information Network (GAIN) genome-wide association study (GWAS) in major depressive disorder (MDD) were used to explore previously reported candidate gene and single-nucleotide polymorphism (SNP) associations in MDD. A systematic literature search of candidate genes associated with MDD in case-control studies was performed before the results of the GAIN MDD study became available. Measured and imputed candidate SNPs and genes were tested in the GAIN MDD study encompassing 1738 cases and 1802 controls. Imputation was used to increase the number of SNPs from the GWAS and to improve coverage of SNPs in the candidate genes selected. Tests were carried out for individual SNPs and the entire gene using different statistical approaches, with permutation analysis as the final arbiter. In all, 78 papers reporting on 57 genes were identified, from which 92 SNPs could be mapped. In the GAIN MDD study, two SNPs were associated with MDD: C5orf20 (rs12520799; P=0.038; odds ratio (OR) AT=1.10, 95% CI 0.95-1.29; OR TT=1.21, 95% confidence interval (CI) 1.01-1.47) and NPY (rs16139; P=0.034; OR C allele=0.73, 95% CI 0.55-0.97), constituting a direct replication of previously identified SNPs. At the gene level, TNF (rs76917; OR T=1.35, 95% CI 1.13-1.63; P=0.0034) was identified as the only gene for which the association with MDD remained significant after correction for multiple testing. For SLC6A2 (norepinephrine transporter (NET)) significantly more SNPs (19 out of 100; P=0.039) than expected were associated while accounting for the linkage disequilibrium (LD) structure. Thus, we found support for involvement in MDD for only four genes. However, given the number of candidate SNPs and genes that were tested, even these significant may well be false positives. The poor replication may point to publication bias and false-positive findings in previous candidate gene studies, and may also be related to heterogeneity of the MDD phenotype as well as contextual genetic or environmental factors.
Asunto(s)
Trastorno Depresivo Mayor/genética , Predisposición Genética a la Enfermedad , Polimorfismo de Nucleótido Simple/genética , Biología Computacional , Frecuencia de los Genes , Estudio de Asociación del Genoma Completo , Genotipo , Humanos , Desequilibrio de Ligamiento , Proteínas del Tejido Nervioso/genética , Neuropéptido Y/genética , Proteínas de Transporte de Noradrenalina a través de la Membrana Plasmática/genética , Oportunidad Relativa , Peptidil-Dipeptidasa A/genética , PubMed/estadística & datos numéricos , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
Symptoms, diagnosis and therapy of equine botulism are discussed by the presentation of two detailed reports of horses with neurological symptoms and the results of laboratory investigations over the period 2003-2008 in the Netherlands. In addition a brief summary of the available literature is presented. Prevailing symptoms of botulism in horses include paralysis of the tongue, salvation, dysphagia and paresis and paralysis of the skeletal muscles, as well as signs of colic. Symptoms and prognosis vary with the amount of botulinum neurotoxin (BoNT) involved. For early clinical diagnosis of botulism thorough investigation of the facial nerves is important, for instance by the use of the 'Tongue Stress Test'. Laboratory results often remain negative, probably due to the sampling time, the high sensitivity of horses for botulinum neurotoxin or treatment with antitoxins. Most clinical cases in horses are caused by botulinum neurotoxin B (BoNT/B). For therapy to be successful antiserum needs to be administered in the earliest possible stage of the disease and this should be supported by symptomatic therapy. Botulism is a feed-related intoxication caused by either carcasses in the roughage or BoNT/B production after poor conservation of grass silage. This is the main source of botulism in horses due to the popularity of individually packed grass silage as feed for horses. As long as no vaccine is available in the Netherlands quality control of silage and haylage is strictly recommended in order to reduce the risk of botulism in horses.
Asunto(s)
Botulismo/veterinaria , Clostridium botulinum tipo B/aislamiento & purificación , Contaminación de Alimentos , Enfermedades de los Caballos/diagnóstico , Animales , Antitoxinas/uso terapéutico , Botulismo/diagnóstico , Botulismo/tratamiento farmacológico , Resultado Fatal , Femenino , Enfermedades de los Caballos/tratamiento farmacológico , Caballos , MasculinoRESUMEN
The USDA germplasm repositories help to preserve the genetic variability of important crop species by collecting and maintaining representative cultivars and related germplasm. Simple sequence repeat markers with high allelic diversity were used to type 41 grapevines from 40 accessions. All vines were either seedless table grape cultivars or cultivars with names similar to table grape cultivars. The proportion of shared alleles was selected as the most appropriate statistical measure of genetic distance for this population. In conjunction with morphological traits, known synonyms were confirmed and a previously unknown synonym was discovered. An alleged synonym in the literature was disproved by the DNA data. The data were consistent with known parentage, where such data were available. Two mislabeled vines in the USDA collection were identified. UPGMA grouped the cultivars loosely into three groups: a group of nine mostly Middle Eastern cultivars, a group of 22 accessions mostly from Russia and Afghanistan that were morphologically similar to 'Thompson Seedless', and a third very loose group of 11 accessions consisting mostly of eastern European wine grape cultivars. The limitations and usefulness of this type of analysis are discussed.
Asunto(s)
Clonación de Organismos , ADN de Plantas/genética , Repeticiones de Microsatélite/genética , Filogenia , Vitis/clasificación , Vitis/genética , Alelos , Variación Genética , Vitis/citología , Vitis/crecimiento & desarrolloRESUMEN
BACKGROUND: The pathway by which Helicobacter pylori induces apoptosis in gastric epithelial cells is not known. The aim of this study was to determine whether H. pylori-induced apoptosis is associated with SAPK/JNK activity in human gastric cancer KATO III cells. MATERIALS AND METHODS: H. pylori VacA toxin positive strain was incubated with KATO III cells for 0.5, 1, 2 or 24 hours. The SAPK/JNK protein was harvested from the KATO III cell lysate by precipitation with a C-jun fusion protein and its activity was measured by C-jun phosphorylation utilizing transblotting and phosphoserine antibody. Cellular apoptosis was demonstrated by DNA fragmentation. In addition, cell growth in coculture with H. pylori was determined over 72 hours. RESULTS: H. pylori significantly stimulated SAPK/JNK activity in KATO III cells with a peak at the 0.5 hour time point (3.6-fold vs. control, p <.05), but a return to basal levels by 2 hours. In addition, significant DNA fragmentation was observed after 24 hours in these cells but not in the control KATO III cells. Cell growth was inhibited in a dose dependent fashion in coculture with H. pylori. CONCLUSION: These results show that H. pylori triggers an increase in apoptosis in KATO III cells as reflected by DNA fragmentation. This effect was preceded and correlated with an increase in SAPK/JNK activity suggesting that the H. pylori-induced apoptosis in human gastric epithelial cells may be mediated by the SAPK/JNK pathway.
Asunto(s)
Apoptosis , Células Epiteliales/microbiología , Mucosa Gástrica/microbiología , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Fragmentación del ADN , Humanos , Transducción de Señal , Células Tumorales CultivadasRESUMEN
It has been demonstrated that human plasma contains a low molecular weight sodium-potassium-stimulated adenosine triphosphatase (Na-K-ATPase) inhibitor, which can be dissociated from a circulating protein with a molecular weight of approximately 12,000 daltons. The dissociated factor was found to have a molecular weight <500 daltons, and shared many characteristics with ouabain. Similar to ouabain, this factor was found to be a potent inhibitor of both the Na-K-ATPase and potassium-stimulated para-nitrophenyl phosphatase (K-pNPPase) enzyme systems, and to bind to both high- and low-affinity binding sites on Na-K-ATPase, but unlike ouabain did not cross-react with digoxin antibody. The factor was further separated by HPLC and electrochemical detection into two active compounds (p-NKAI-1 and p-NKAI-2). P-NKAI-1 was demonstrated on mass spectroscopy to have a molecular weight of 408 daltons. In a vasoconstrictor assay employing rabbit femoral artery segments, this compound was a direct vasoconstrictor and potentiated the vasoconstriction produced by norepinephrine. It behaved similarly to ouabain in counteracting the relaxing effect on rabbit femoral artery of increasing potassium concentrations in the tissue bath.
Asunto(s)
Proteínas Sanguíneas/aislamiento & purificación , Inhibidores Enzimáticos/sangre , Hipertensión/sangre , ATPasa Intercambiadora de Sodio-Potasio/antagonistas & inhibidores , 4-Nitrofenilfosfatasa/antagonistas & inhibidores , Animales , Unión Competitiva , Proteínas Sanguíneas/química , Digoxina/inmunología , Electroforesis en Gel de Poliacrilamida , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/farmacología , Arteria Femoral/efectos de los fármacos , Arteria Femoral/fisiología , Humanos , Técnicas In Vitro , Masculino , Espectrometría de Masas , Peso Molecular , Ouabaína/metabolismo , Ouabaína/farmacología , Conejos , Radioinmunoensayo , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Tritio , Vasoconstricción/efectos de los fármacosRESUMEN
The aim of this study was to investigate whether IGF I induction of p53 expression and p21 promoter require activation of MAP kinase in cardiac muscle cells. Compared to cardiomyocytes transfected with control vector, activation of MAP kinase by IGF I was decreased by approximately 60-70% in the cells transfected with dominant negative MAP kinase Y185. Transfection with Y185 also resulted in decreased induction of p53 mRNA by IGF I (70% reduction). In the cells transfected with a wildtype p21WAF1/CIP1 promoter construct, activation of luciferase reporter gene by IGF I was decreased in the cells co-transfected with Y185. To further confirm these findings, cells were preincubated with PD98059, a specific MAP kinase kinase inhibitor. As expected, PD98059 inhibited induction of p53 mRNA and p21WAF1/CIP1 promoter by IGF I. These data indicate that transcriptional activation of p53 and p21WAF1/CIP1 by IGF I involves MAP kinase pathway in cardiomyocytes, and thus link MAP kinase to negative modulation of the cell cycle in cardiac muscle cells.
Asunto(s)
Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Factor I del Crecimiento Similar a la Insulina/farmacología , Miocardio/enzimología , Proteína p53 Supresora de Tumor/biosíntesis , Animales , Proteínas Quinasas Dependientes de Calcio-Calmodulina/antagonistas & inhibidores , Células Cultivadas , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , Ciclinas/biosíntesis , Ciclinas/genética , Activación Enzimática , Inhibidores Enzimáticos/farmacología , Flavonoides/farmacología , Corazón/efectos de los fármacos , Regiones Promotoras Genéticas , Ratas , Transducción de Señal , Proteína p53 Supresora de Tumor/genéticaRESUMEN
Important vascular proteins such as endothelin-1 (ET-1) promote the development of cardiovascular diseases. Oestrogen, and perhaps progesterone, prevent the development of vascular disease in women through incompletely understood cellular mechanisms. We hypothesized that oestradiol or progesterone might regulate the production of ET-1 as a potential novel mechanism. We found that serum and angiotensin II (AII) significantly stimulated ET-1 secretion from cultured bovine aortic endothelial cells, inhibited 50-75% by oestradiol or by progesterone. Serum and AII stimulated ET-1 mRNA levels, inhibited at least 70% by oestradiol and by progesterone. Serum stimulated ET-1 transcription mainly through the first 43 nucleotides of the ET-1 promoter, but oestradiol and progesterone did not inhibit this. In contrast, AII stimulated ET-1 transcription through nucleotides -143 to -98, specifically involving an activator protein-1 (AP-1) site at -102. Oestradiol and progesterone caused a 60-70% inhibition of AII-stimulated wild-type construct -. 143ET-1/CAT activity (CAT is chloramphenicol acyltransferase). AII-stimulation of ET-1 transcription was critically dependent on stimulation of mitogen-activated protein kinase (erk) activity, inhibited by oestradiol and progesterone. In summary, we found that sex steroids inhibit AII-induced erk signalling to the ET-1 transcriptional programme. This novel mechanism of negative transcriptional regulation by oestradiol and progesterone decreases the production of ET-1, potentially contributing to the vascular protective effects of these steroids.
Asunto(s)
Endotelina-1/biosíntesis , Endotelio Vascular/efectos de los fármacos , Estradiol/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Progesterona/farmacología , Transcripción Genética , Angiotensina II , Animales , Aorta , Bovinos , Células Cultivadas , Cloranfenicol O-Acetiltransferasa/biosíntesis , Medios de Cultivo , Medio de Cultivo Libre de Suero , Endotelina-1/genética , Endotelio Vascular/citología , Endotelio Vascular/metabolismo , Femenino , Semivida , Humanos , Regiones Promotoras Genéticas , Biosíntesis de Proteínas , ARN Mensajero/metabolismo , Proteínas Recombinantes de Fusión/biosíntesis , Transcripción Genética/efectos de los fármacos , TransfecciónRESUMEN
Estrogen (E) has been identified in epidemiologic and prospective studies to protect against the development of cardiovascular disease in women. It is unclear whether progesterone (P) is similarly beneficial. The mechanisms by which E or P might act are incompletely defined. One possibility is that sex steroids inhibit the proliferation of vascular smooth muscle, an early/important event in vascular pathology. We examined the ability of E and P to inhibit the growth of human umbilical vein smooth muscle cells (hUVSMC) in culture, when stimulated by serum or the mitogen, endothelin-1 (ET-1). Serum and ET-1 stimulated hVSMC cell numbers by approximately 110% and 43% respectively, compared with control, after 3 days in culture. This stimulation was maximally reversed 75% by E and 64% by P. No synergistic or additive effects of the two steroids were found. ET-1 and serum stimulated mitogen-activated protein kinase (MAP-K) and MAP-kinase kinase activities, and these were critical for mitogenesis. Mitogen-stimulated MAP-kinase kinase and MAP-K activities were significantly inhibited by either E or P. The steroids also inhibited mitogen-stimulated c-fos and c-myc, downstream targets for MAP-K action. Critical signaling and molecular events through which mitogens stimulate VSMC proliferation can be significantly inhibited by E or P, providing a potential cellular mechanism for their vascular protective actions.
Asunto(s)
Estrógenos/farmacología , Músculo Liso Vascular/citología , Músculo Liso Vascular/efectos de los fármacos , Progesterona/farmacología , Northern Blotting , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Proteínas Quinasas Dependientes de Calcio-Calmodulina/fisiología , División Celular/efectos de los fármacos , División Celular/fisiología , Células Cultivadas , ADN/metabolismo , Endotelina-1/farmacología , Activación Enzimática , Femenino , Regulación de la Expresión Génica/efectos de los fármacos , Genes fos/genética , Genes myc/genética , Humanos , Quinasas de Proteína Quinasa Activadas por Mitógenos , Mitógenos/farmacología , Músculo Liso Vascular/química , Embarazo , Proteínas Quinasas/metabolismo , Proteínas Quinasas/fisiología , Proteínas Proto-Oncogénicas c-fos/análisis , Proteínas Proto-Oncogénicas c-fos/genética , Proteínas Proto-Oncogénicas c-fos/metabolismo , Proteínas Proto-Oncogénicas c-myc/análisis , Proteínas Proto-Oncogénicas c-myc/genética , Proteínas Proto-Oncogénicas c-myc/metabolismo , Albúmina Sérica Bovina/farmacología , Timidina/metabolismo , Tritio , Venas Umbilicales/química , Venas Umbilicales/citología , Venas Umbilicales/efectos de los fármacosRESUMEN
The toxicity of aziridinylbenzoquinones may occur by a number of mechanisms, including oxidative stress caused by redox cycling and the activation of the aziridine groups. Isolated hepatocytes were used to assess the relationship between the redox status of NADP(H) associated with oxidative stress, the level of NAD(H) closely linked with DNA repair and the cytotoxicity of three 2,5-bis(aziridinyl)-1,4-benzoquinones (BABQ). Exposure of hepatocytes to the BABQ TW13 (200 microM) and TW25 (100 microM), which are able to arylate and to redox cycle, resulted in increased intracellular NADP+ from < 0.3 nmol/mg protein to 1.5 nmol/mg protein within 60 min. The increase in intracellular NADP+ was followed by the onset of cell death by 180 min. In contrast, exposure to lower concentrations of TW13 (100 microM), TW25 (50 microM) and carboquone (100-200 microM) (which neither arylates nor redox cycles via a one-electron reduction) resulted in a less pronounced (< 1.0 nmol/mg) increase in NADP+ and there was no evidence of cell death within the 180 min incubation. BABQ had a concentration dependent effect on intracellular NAD+. Exposure of hepatocytes to TW13 (200 microM) and TW25 (100 microM) resulted in a decrease in intracellular NAD+ from > 2.7 to < 1.0 nmol/mg protein within 60 min. At concentrations of the BABQ where the level of NAD+ remained > 1.0 nmol/mg protein after 30 min, the hepatocytes remained viable at 180 min. These changes in intracellular pyridine nucleotides suggests two mechanisms may be involved in BABQ cytotoxicity. At high concentrations, aziridinylbenzoquines may cause cytotoxicity via oxidative stress following redox cycling. At lower concentrations however, the predominant pyridine nucleotide change is a prolonged depletion of NAD+, suggesting extensive DNA damage which may lead to delayed cell death.
Asunto(s)
Aziridinas/toxicidad , Benzoquinonas/toxicidad , Hígado/efectos de los fármacos , NADH NADPH Oxidorreductasas/metabolismo , Adenosina Trifosfato/metabolismo , Animales , Aziridinas/química , Benzoquinonas/química , Supervivencia Celular/efectos de los fármacos , Células Cultivadas/efectos de los fármacos , Hígado/enzimología , Masculino , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas WistarRESUMEN
The modulation of the activity of mitogen-activated protein kinase (MAPK) by endogenous growth factors or growth inhibitors provides a potential means of regulating cell proliferation. We determined the effect of the endogenous anti-proliferative peptide, atrial natriuretic peptide (ANP), on the ability of MAPK to phosphorylate myelin basic protein. In astrocytes, MAPK activity was significantly stimulated (up to 3-fold) by three known glial mitogens, endothelin-3, platelet-derived growth factor, or phorbol 12-myristate 13-acetate. ANP inhibited by 55-70% the ability of each of these mitogens to activate MAPK. The effects of ANP were equipotent to those caused by C-ANP 4-23, a peptide that specifically binds to the natriuretic peptide clearance receptor. Additionally, both natriuretic peptides caused a 70-80% inhibition of the sodium vanadate-stimulated MAPK activity, complete inhibition of the okadaic acid-stimulated activity, and inhibition of the mitogen-stimulated phosphorylation of MAPK. To understand the potential mechanism by which the natriuretic peptides act, we found that both ANP and C-ANP inhibited the mitogen-stimulated activity of the immediate upstream kinase in the cascade, MAPK kinase (MEK). C-ANP also strongly inhibited the endothelin-3-, platelet-derived growth factor-, and phorbol 12-myristate 13-acetate-induced stimulation of DNA synthesis in the astrocytes, while both okadaic acid and sodium vanadate significantly reversed these anti-proliferative actions. Our results identify ANP as a peptide hormone that inhibits growth factor-stimulated MAPK. These data suggest that the ability of the natriuretic peptides to inhibit MAPK may be important for their anti-growth actions. This effect likely occurs via the inhibition of upstream kinase(s), including MEK, uniquely resulting from ligand binding to the natriuretic peptide clearance receptor.
Asunto(s)
Factor Natriurético Atrial/farmacología , Proteínas Quinasas Dependientes de Calcio-Calmodulina/antagonistas & inhibidores , Diencéfalo/citología , Receptores del Factor Natriurético Atrial/fisiología , Animales , Astrocitos/citología , Astrocitos/efectos de los fármacos , Astrocitos/metabolismo , División Celular/efectos de los fármacos , Células Cultivadas , Endotelinas/farmacología , Activación Enzimática , Inhibidores Enzimáticos/farmacología , Éteres Cíclicos/farmacología , Feto , Cinética , Ácido Ocadaico , Fosforilación , Factor de Crecimiento Derivado de Plaquetas/farmacología , Ratas , Receptores del Factor Natriurético Atrial/efectos de los fármacos , Acetato de Tetradecanoilforbol/farmacología , Vanadatos/farmacologíaRESUMEN
Inhibitors of sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) have been implicated in the pathogenesis of hypertension. In the study presented here, an attempt was made to determine whether differences in the plasma levels and the removal rates of high-molecular weight (HMW) and low-molecular weight (LMW) forms of Na-K-ATPase inhibitors might relate to blood-pressure control in hemodialysis (N = six ultrafiltered and N = six non-ultrafiltered) and CAPD (N = six long-term and N = five short-term) patients. The latter group was studied before the initiation of continuous ambulatory peritoneal dialysis (CAPD) and 2 wk after starting the treatment. The mean blood pressure was significantly reduced after dialysis in the nonultrafiltered hemodialysis group and in both CAPD groups. Plasma levels of both HMW and LMW inhibitors were found to be elevated before dialysis in all patients and were modified only slightly after dialysis. Irrespective of whether ultrafiltration was utilized in hemodialysis patients and despite significant losses of both HMW and LMW inhibitors into CAPD effluent. Because CAPD effluent was found to contain vasopressors that were not exclusively Na-K-ATPase inhibitors, losses of these other vasopressors may contribute to improved blood-pressure control in CAPD in contrast to hemodialysis.
Asunto(s)
Presión Sanguínea/fisiología , Inhibidores Enzimáticos/metabolismo , Fallo Renal Crónico/metabolismo , Diálisis Peritoneal Ambulatoria Continua , Diálisis Renal , ATPasa Intercambiadora de Sodio-Potasio/antagonistas & inhibidores , Cromatografía Líquida de Alta Presión , Soluciones para Diálisis/metabolismo , Electroforesis en Gel de Poliacrilamida , Humanos , Fallo Renal Crónico/fisiopatología , Fallo Renal Crónico/terapia , Peso Molecular , Vasoconstricción , Vasoconstrictores/metabolismoRESUMEN
Natriuretic peptides are produced in the brain, heart and vasculature, and cause vasodilation, sodium excretion, and diuresis. Recent advances indicate that they play important roles in blood-pressure homeostasis, both in normal and in pathophysiological conditions. Although therapeutic interventions which elevate plasma natriuretic peptide levels do not have great antihypertensive efficacy, animal studies suggest that they may be useful in combination treatment strategies.
Asunto(s)
Factor Natriurético Atrial/fisiología , Hipertensión/fisiopatología , Proteínas del Tejido Nervioso/fisiología , Proteínas/fisiología , Animales , Factor Natriurético Atrial/metabolismo , Humanos , Hipertensión/metabolismo , Péptido Natriurético Encefálico , Péptido Natriurético Tipo-C , Proteínas del Tejido Nervioso/metabolismo , Proteínas/metabolismo , Receptores de Péptidos/metabolismoRESUMEN
C-type natriuretic peptide (CNP) is a member of the natriuretic peptide family which is produced in vascular endothelial cells and may play an important paracrine role in the vasaculature. We sought to determine the regulation of CNP production by other vasoactive peptides from cultured aortic endothelial cells. The vasoconstrictors endothelin-1 and angiotensin II had little effect on the basal secretion of CNP. In contrast, atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) strongly stimulated the secretion of CNP. BNP caused as much as a 400-fold enhancement above the basal accumulated secretion of CNP over 24 h at a concentration of 1 microM; this was 20 times greater than the stimulatory effect of ANP, BNP and ANP also significantly enhanced the production of new CNP protein (translation) and mRNA expressed in the BAEC. In contrast, C-ANP-4-23, a truncated form of ANP which selectively binds to the natriuretic peptide clearance receptor, did not stimulate CNP secretion. The enhanced production and secretion of CNP, caused by either ANP or BNP, was significantly prevented by LY 83583, an inhibitor of cGMP generation, and was also attenuated by KT 5823, an inhibitor of cGMP-dependent protein kinase. Our results indicate that ANP and BNP can stimulate CNP production through a guanylate cyclase receptor on endothelial cells. BNP is a much more potent stimulator of CNP secretion, compared to ANP. Our findings suggest that the vasodilatory, and anti-mitogenic effects of ANP and BNP in the vasculature could occur in part through CNP production and subsequent action if these interactions occur in vivo.
Asunto(s)
Factor Natriurético Atrial/farmacología , Endotelio Vascular/metabolismo , Proteínas del Tejido Nervioso/farmacología , Proteínas/metabolismo , Angiotensina II/farmacología , Animales , Aorta/citología , Bovinos , Células Cultivadas , Endotelinas/farmacología , Endotelio Vascular/efectos de los fármacos , Regulación de la Expresión Génica , Péptido Natriurético Encefálico , Péptido Natriurético Tipo-C , Biosíntesis de Proteínas , Proteínas/genética , ARN Mensajero/análisisRESUMEN
Substantial evidence from animal studies suggests that enhanced memory associated with emotional arousal results from an activation of beta-adrenergic stress hormone systems during and after an emotional experience. To examine this implication in human subjects, we investigated the effect of the beta-adrenergic receptor antagonist propranolol hydrochloride on long-term memory for an emotionally arousing short story, or a closely matched but more emotionally neutral story. We report here that propranolol significantly impaired memory of the emotionally arousing story but did not affect memory of the emotionally neutral story. The impairing effect of propranolol on memory of the emotional story was not due either to reduced emotional responsiveness or to nonspecific sedative or attentional effects. The results support the hypothesis that enhanced memory associated with emotional experiences involves activation of the beta-adrenergic system.
Asunto(s)
Emociones/fisiología , Memoria/fisiología , Receptores Adrenérgicos beta/fisiología , Adulto , Presión Sanguínea/efectos de los fármacos , Femenino , Frecuencia Cardíaca/efectos de los fármacos , Humanos , Masculino , Memoria/efectos de los fármacos , PropranololRESUMEN
An elevation in mean blood pressure was found in rats treated with low lead (0.01%) for 6 months and then only water for an additional 6 months (discontinuous low lead). No change in blood pressure was found in rats similarly treated with high lead (0.5%) (discontinuous high lead). Administration of DMSA (0.5% in drinking water), for 5 days every 2 months following cessation of lead administration, resulted in a significant lowering of blood pressure in both groups of animals. In the low-lead but not the high-lead group, this was associated with an increase in plasma cyclic GMP (acting as a second messenger for endothelium-derived relaxing factor, EDRF) and a decrease in the plasma concentration of a 12-kDa hypertension-associated protein. Plasma endothelin-3 (ET-3) levels were decreased in discontinuous high-lead rats, increased in discontinuous low-lead rats, but were unaltered by DMSA treatment. We infer that the elevated blood pressure in the discontinuous low-lead rats is related to an increase in the putative vasoconstrictors, ET-3 and the hypertension-associated protein, without a change in the vasodilator, EDRF. With DMSA treatment, plasma cyclic GMP in low-lead rats increased above normal, and the hypertension-associated protein decreased, resulting in lowered blood pressure. DMSA was shown to act as an antioxidant in vitro. Thus the DMSA effect on plasma cGMP (EDRF) may occur via a scavenging effect on EDRF-inactivating reactive oxygen species.
Asunto(s)
Presión Sanguínea/efectos de los fármacos , Terapia por Quelación , Plomo , Succímero/farmacología , Animales , GMP Cíclico/sangre , Endotelinas/sangre , Masculino , Ratas , Ratas Sprague-Dawley , Succímero/uso terapéuticoRESUMEN
Endothelin-1 (ET-1) is the most potent endogenous vasoconstrictor yet identified. This peptide plays an important role in the regulation of arterial tone, in part through its interaction with endogenous vasodilator compounds. To understand the interactions of endothelin with the vasoactive prostaglandins (PGs), we determined the effects of prostaglandin E2 (PGE2), prostacyclin (PGI2), and thromboxane A2 on ET-1 synthesis and secretion from cultured bovine aortic endothelial cells and on ET-1 action in aortic smooth muscle cells. Both PGE2 and PGI2 (vasodilator prostaglandins) caused an approximately 40% inhibition of basal ET-1 secretion and a 50% inhibition of serum-stimulated ET-1 secretion in a dose-related and time course fashion. In contrast, the vasoconstrictor prostaglandin, thromboxane A2, had no effect on ET-1 secretion. PGE2 and PGI2 similarly inhibited the basal production of new ET-1 protein (translation) by 40-50% and inhibited the basal steady-state mRNA expression of ET-1 in bovine aortic endothelial cells by 60-70%. Both prostaglandins also caused an approximately 55% inhibition of ET-1 transcription, as shown by chloramphenicol acetyltransferase reporter studies. PGE2 and PGI2 strongly stimulated cGMP generation; both the PG stimulation of cGMP and the inhibition of ET-1 secretion and translation were reversed by LY83583, a general inhibitor of cGMP generation. The PG-induced inhibition of ET-1 secretion and translation was also reversed by KT5823, an inhibitor of cGMP-dependent protein kinase, but not by (Rp)-adenosine cyclic 3':5'-monophosphate, an inhibitor of protein kinase A activation. PGE2 and PGI2 also inhibited both basal and ET-1-stimulated DNA synthesis in aortic smooth muscle cells by approximately 45% through a cGMP-dependent mechanism. Therefore, two endogenous PGs, known to be important vasodilators in vivo, significantly inhibit the transcription, translation, secretion, and action of ET-1. We propose that the vasodilator action of the PGs results, in part, from their ability to inhibit the production of this potent vasoconstrictor.
Asunto(s)
Aorta/fisiología , Carbazoles , Dinoprostona/farmacología , Endotelinas/biosíntesis , Endotelinas/farmacología , Endotelio Vascular/metabolismo , Epoprostenol/farmacología , Indoles , Músculo Liso Vascular/fisiología , ARN Mensajero/metabolismo , Alcaloides/farmacología , Aminoquinolinas/farmacología , Animales , Aorta/efectos de los fármacos , Bovinos , División Celular/efectos de los fármacos , Células Cultivadas , Cloranfenicol O-Acetiltransferasa/biosíntesis , Cloranfenicol O-Acetiltransferasa/metabolismo , GMP Cíclico/metabolismo , ADN/biosíntesis , Relación Dosis-Respuesta a Droga , Endotelinas/metabolismo , Endotelio Vascular/citología , Endotelio Vascular/efectos de los fármacos , Cinética , Músculo Liso Vascular/efectos de los fármacos , Biosíntesis de Proteínas , Inhibidores de Proteínas Quinasas , SRS-A/antagonistas & inhibidores , Timidina/metabolismo , TransfecciónRESUMEN
The concentration of HDL in the blood inversely correlates with the incidence of cardiovascular disease, probably related to the ability of these lipoproteins to efflux cholesterol from vascular cells. it is also possible that HDL affect the production or action of vasoactive peptides implicated in the development of vascular diseases. Therefore, we determined the effects of human HDL on the production and secretion of endothelin-1 (ET-1) from cultured bovine aortic endothelial cells. HDL produced a highly significant stimulation of endothelin secretion (maximum 240% of control), even at very low levels of lipoproteins (1 microgram/ml). HDL also stimulated the translation of ET-1 by twofold in the bovine aortic endothelial cells. In contrast, HDL had no significant effect on steady state mRNA levels, transcript degradation, or transcription. Stimulation of ET-1 secretion by HDL was dependent on protein kinase C activation. Purified apo A-I, the major apoprotein of HDL, increased ET-1 secretion and translation approximately 85% as potently as HDL. Our results indicate that low concentrations of human HDL strongly stimulate the production of ET-1, a powerful vasoconstrictor and mitogen for the vascular smooth muscle cell. We propose that HDL may participate in the regulation of vasomotor tone through this potentially important effect in the vasculature.
Asunto(s)
Endotelinas/biosíntesis , Endotelio Vascular/metabolismo , Lipoproteínas HDL/farmacología , Animales , Aorta , Bovinos , Células Cultivadas , Endotelinas/genética , Endotelio Vascular/efectos de los fármacos , Proteína Quinasa C/fisiología , ARN Mensajero/análisisRESUMEN
It has been demonstrated that expansion of extracellular fluid volume induces the release of a low-molecular-weight natriuretic and sodium-potassium-activated adenosine triphosphatase inhibiting hormone (NKAI). In this study, we used a highly purified hormone extracted from pooled hypertensive urines (u-NKAI). Like ouabain, this compound was found to be a potent inhibitor of the sodium-potassium-activated adenosine-triphosphatase and potassium-stimulated paranitrophenyl phosphatase enzyme systems as well as a vasoconstrictor in vitro. In contrast to ouabain, which is a competitive inhibitor of both enzyme systems with respect to potassium, u-NKAI is noncompetitive. Furthermore, u-NKAI differs from ouabain by its lack of cross-reactivity with digoxin antibodies. In addition, whereas ouabain binds to both high-affinity and low-affinity binding sites on the sodium-potassium-activated adenosine-triphosphatase enzyme in the absence of potassium, u-NKAI binds only to the low-affinity binding sites. This study demonstrates that the highly purified u-NKAI, although ouabain-like in certain respects, is not an "endogenous ouabain."
Asunto(s)
Inhibidores Enzimáticos/aislamiento & purificación , Natriuréticos/aislamiento & purificación , ATPasa Intercambiadora de Sodio-Potasio/antagonistas & inhibidores , 4-Nitrofenilfosfatasa/antagonistas & inhibidores , Animales , Cromatografía Líquida de Alta Presión , Inhibidores Enzimáticos/orina , Humanos , Cinética , Masculino , Peso Molecular , Natriuréticos/farmacología , Natriuréticos/orina , Ouabaína/farmacología , Potasio/farmacología , ConejosRESUMEN
This study was performed to establish relationships between the structure of 2,5-bis(1-aziridinyl)-1,4-benzoquinones (BABQs) bearing different substituents at the 3- and 6-position and their acute toxic effects in rat hepatocytes. The cell viability, loss of cellular glutathione (GSH+GSSG) and loss of ATP were followed during 4 h of incubation of freshly isolated hepatocytes. The toxicity of these compounds (100 microM) was predicted better by their reactivity with GSH than by their redox cycling in rat liver microsomes. The time of 50% loss of viability (LT50) correlated very well with the time of 50% depletion of ATP (AT50). LT50 could be adequately predicted by using the electronic field parameter (Ftotal) describing the electron withdrawing or donating properties for all the substituents on the quinone-nucleus. 7-(Di)halogen-substituted BABQs that all very rapidly depleted cellular glutathione showed significant differences in AT50 as well as in LT50. This suggests that alterations in ATP levels are important for explaining the differences in cytotoxicity of these compounds.