RESUMEN
(S)-1-(1-naphthyl) ethanol (SNE) is a chiral drug intermediate for the production of mevinic acid analog, a potent cholesterol agent. It acts as an HMG-CoA reductase inhibitor and is hence used in the synthesis of statins. Statins are lipid-lowering drugs used to lower cholesterol in the body. In our present study, we carried out whole-cell bioreduction of 1-Acetonaphthone to enantiopure SNE using selected microorganisms acquired by soil acclimation technique. The microorganism which exhibited higher bioreduction activity was determined using high-performance liquid chromatography (HPLC), and it was identified as Pichia kudriavzevii by ITS primer sequencing. After optimizing the parameters, Pichia sp. produced SNE with good conversion (75%), yield (67%), and excellent enantiomeric excess (100%). The microbial enzyme showed higher activity at 24-h-old supernatant. The crude and partially purified enzyme exhibited a specific activity of 51.13 U/mL and 62.72 U/mL, respectively, with a 1.22 purification fold.
Asunto(s)
Inhibidores de Hidroximetilglutaril-CoA Reductasas , Etanol/química , ColesterolRESUMEN
An assay method for detection of enantiospecific chiral alcohol was developed based on ketoreductase, enantio-selective alcohol oxidase and 2,4-dinitrophenyl hydrazine (DNPH) reagent. The assay method was developed to check the conversion of 1-Acetonapthone to either (S) or (R) specific 1-(1-napthyl) ethanol or its racemic mixture using ketoreductases. Further, estimation was done with the help of 2,4-DNPH method. The resulting orange coloured chromogen showed a maximum absorbance at 560nm. The assay was performed in 96 well microtiter plates and had a linear detection range from 0.05mM to 4mM. The method is found to be suitable for the detection of large numbers of crude samples and screening of ketoreductase producing strains in high-throughput manner.