RESUMEN
Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism. Here, comparative studies of two phenotypic methods using the tube method and the spectrophotometric method for slime production from 100 clinically and 21 environmentally significant isolates of P. aeruginosa were performed. A total of 68 isolates were positive by either of the tests whereas only 34 were positive by both the tests. The tube method detected slime significantly in more number of isolates than the spectrophotometric method. The tube test was found to be superior to the spectrophotometric method in ease of performance, interpretation and sensitivity. Among the clinical isolates, systemic isolates produce less slime compared to wound, respiratory and urinary isolates. Isolates from the hospital environment produced more slime indicating that this virulence marker helps the organism to survive for longer periods and cause nosocomial infections.
Asunto(s)
Técnicas Bacteriológicas/métodos , Microbiología Ambiental , Polisacáridos Bacterianos/biosíntesis , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/metabolismo , Factores de Virulencia/biosíntesis , Humanos , Pseudomonas aeruginosa/aislamiento & purificación , VirulenciaRESUMEN
An attempt to induce resistance to ciprofloxacin in vitro was made against clinical and environmental isolates of Pseudomonas aeruginosa. This in vitro manipulation of strains resulted in the increase of minimum inhibitory concentration from 0.4 microg/ml to 1 microg/ml of the original strains to 9.0 to 12.5 microg/ml indicating development of resistance to ciprofloxacin and a major decrease in the size of zone diameters of norfloxacin and ofloxacin indicating cross resistance to these agents. Results indicate the induced resistance to ciprofloxacin can promote development of cross resistance to other fluoroquinolones. This suggests that caution should be taken while using fluoroquinolones for the treatment of Pseudomonas aeruginosa infections.