RESUMEN

The hydrolysis of sucrose, the principal dietary source of carbon for aphids, is catalysed by a gut alpha-glucosidase/transglucosidase activity. An alpha-glucosidase, referred to as APS1, was identified in both a gut-specific cDNA library and a sucrase-enriched membrane preparation from guts of the pea aphid Acyrthosiphon pisum by a combination of genomic and proteomic techniques. APS1 contains a predicted signal peptide, and has a predicted molecular mass of 68 kDa (unprocessed) or 66.4 kDa (mature protein). It has amino acid sequence similarity to alpha-glucosidases (EC 3.2.1.20) of glycoside hydrolase family 13 in other insects. The predicted APS1 protein contains two domains: an N-terminal catalytic domain, and a C-terminal hydrophobic domain. In situ localisation and RT-PCR studies revealed that APS1 mRNA was expressed in the gut distal to the stomach, the same localisation as sucrase activity. When expressed heterologously in Xenopus embryos, APS1 was membrane-bound and had sucrase activity. It is concluded that APS1 is a dominant, and possibly sole, protein mediating sucrase activity in the aphid gut.

Asunto(s)

Áfidos/enzimología , Sacarasa/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Expresión Génica , Intestinos/enzimología , Proteínas de la Membrana/metabolismo , Datos de Secuencia Molecular , Pisum sativum/parasitología , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , alfa-Glucosidasas/metabolismo

RESUMEN

The myogenic regulatory genes MyoD and Myf5 are members of the bHLH transcription factor superfamily. These genes are expressed as an early response to mesoderm induction in the frog Xenopus laevis. This paper describes our work to determine the conservation of sequence, expression and function of the early myogenic genes in the closely related diploid species Xenopus tropicalis. To this end we have cloned and sequenced Xenopus tropicalis homologues of Myf5 and MyoD and found a high degree of conservation in the nucleotide and amino acid sequences of both genes. The expression of Myf5 and MyoD in Xenopus tropicalis was assayed by in situ hybridisation and the expression patterns were found to be similar to those described in Xenopus laevis. The bHLH myogenic regulatory factors are known to be able to auto- and cross-activate expression of the myogenic genes. Interestingly, however, we found that while injection of mRNA coding for either Xenopus tropicalis myf5 (Xtmyf5) or Xenopus tropicalis myoD (XtmyoD) was capable of activating expression of the endogenous Xenopus laevis myoD (XmyoD) and cardiac actin genes, neither was capable of inducing expression from the endogenous Xenopus laevis (Xmyf5) gene.

Asunto(s)

Proteínas de Unión al ADN , Proteínas Musculares/genética , Proteína MioD/genética , Transactivadores , Proteínas de Xenopus/genética , Xenopus/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , Datos de Secuencia Molecular , Proteínas Musculares/química , Proteínas Musculares/metabolismo , Proteína MioD/química , Proteína MioD/metabolismo , Factor 5 Regulador Miogénico , Alineación de Secuencia , Xenopus/embriología

RESUMEN

The use of a novel inducible FGF signalling system in the frog Xenopus laevis is reported. We show that the lipophilic, synthetic, dimerizing agent AP20187 is able to rapidly activate signalling through an ectopically expressed mutant form of FGFR1 (iFGFR1) in Xenopus embryos. iFGFR1 lacks an extracellular ligand binding domain and contains an AP20187 binding domain fused to the intracellular domain of mouse FGFR1. Induction of signalling by AP20187 is possible until at least early neurula stages, and we demonstrate that ectopically expressed iFGFR1 protein persists until late neurula stages. We show that activation of signalling through iFGFR1 can mimic a number of previously reported FGF activities, including mesoderm induction, repression of anterior development, and neural posteriorization. We show that competence to morphological posteriorization of the anteroposterior axis by FGF signalling only extends until about stage 10.5. We demonstrate that the competence of neural tissue to express the posterior markers Hoxa7 and Xcad3, in response to FGF signalling, is lost by the end of gastrula stages. We also show that activation of FGF signalling stimulates morphogenetic movements in neural tissue until at least the end of the gastrula stage.

Asunto(s)

Factores de Crecimiento de Fibroblastos/fisiología , Tacrolimus/análogos & derivados , Proteínas de Xenopus , Xenopus laevis/embriología , Xenopus laevis/fisiología , Animales , Blástula/metabolismo , Tipificación del Cuerpo , Dimerización , Proteínas Fetales/genética , Factores de Crecimiento de Fibroblastos/genética , Gástrula/metabolismo , Proteínas de Homeodominio/genética , Mutación , Proteínas de Neoplasias/genética , Sistema Nervioso/embriología , Fenotipo , Proteínas Tirosina Quinasas Receptoras/química , Proteínas Tirosina Quinasas Receptoras/genética , Proteínas Tirosina Quinasas Receptoras/metabolismo , Receptor Tipo 1 de Factor de Crecimiento de Fibroblastos , Receptores de Factores de Crecimiento de Fibroblastos/química , Receptores de Factores de Crecimiento de Fibroblastos/genética , Receptores de Factores de Crecimiento de Fibroblastos/metabolismo , Transducción de Señal/efectos de los fármacos , Tacrolimus/farmacología , Xenopus laevis/genética

RESUMEN

The caudal gene codes for a homeodomain transcription factor that is required for normal posterior development in Drosophila. In this study the biological activities of the Xenopus caudal (Cdx) family member Xcad3 are examined. A series of domain-swapping experiments demonstrate that the N-terminus of Xcad3 is necessary for it to activate Hox gene expression and that this function can be replaced by the activation domain from the viral protein VP16. In addition, experiments using an Xcad3 repressor mutant (XcadEn-R), which potently blocks the activity of wild-type Xcad3, are reported. Overexpression of XcadEn-R in embryos inhibits the activation of the same subset of Hox genes that are activated by wild-type Xcad3 and leads to a dramatic disruption of posterior development. We show that Xcad3 is an immediate early target of the FGF signalling pathway and that Xcad3 posteriorizes anterior neural tissue in a similar way to FGF. Furthermore, Xcad3 is required for the activation of Hox genes by FGFs. These data provide strong evidence that Xcad3 is required for normal posterior development and that it regulates the expression of the Hox genes downstream of FGF signalling.

Asunto(s)

Proteínas Fetales/metabolismo , Genes Homeobox/fisiología , ARN Mensajero/metabolismo , Proteínas de Xenopus , Animales , Regulación del Desarrollo de la Expresión Génica , Genes Homeobox/genética , Transducción de Señal , Activación Transcripcional , Xenopus/embriología

RESUMEN

We have shown previously that fibroblast growth factor (FGF) signalling in posterior regions of the Xenopus embryo is required for the development of the trunk and tail via a molecular pathway that includes the caudal-related gene Xcad3 and the posterior Hox genes [1]. These results have been contested by the work of Kroll and Amaya [2], which shows that Xenopus embryos transgenic for a dominant-negative form of the FGF receptor (FGF-RI) express posterior Hox genes normally, leading these authors to suggest that the FGFs are not required for anteroposterior (A-P) patterning of the dorsal axis. In order to investigate the apparent discrepancy between these studies, we have produced Xenopus embryos transgenic for two inhibitors of the FGF/Caudal pathway: a kinase-deficient dominant-negative FGF receptor (XFD) [3]; and a domain-swapped form of Xcad3 (Xcad-EnR) in which the activation domain of Xcad3 is replaced by the repression domain of the Drosophila Engrailed protein. Both of these were introduced as fusions with the green fluorescent protein (GFP), which allows identification of non-mosaic transgenic embryos at early gastrula stages by simply looking for GFP fluorescence. Analysis of gene expression in embryos transgenic for these constructs indicated that the activation of posterior Hox genes during early neurula stages absolutely requires FGF signalling and transcriptional activation by Xcad3, while the maintenance of Hox gene expression in the trunk and tail during later development is independent of both FGF and Xcad.

Asunto(s)

Regulación del Desarrollo de la Expresión Génica , Genes Homeobox , Proteínas Tirosina Quinasas Receptoras , Proteínas de Xenopus , Xenopus/embriología , Xenopus/genética , Animales , Animales Modificados Genéticamente , Drosophila/genética , Proteínas de Drosophila , Proteínas Fetales/genética , Proteínas Fetales/metabolismo , Proteínas Fluorescentes Verdes , Proteínas de Homeodominio/genética , Proteínas de Homeodominio/metabolismo , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Mutación , Receptor Tipo 1 de Factor de Crecimiento de Fibroblastos , Receptores de Factores de Crecimiento de Fibroblastos/genética , Receptores de Factores de Crecimiento de Fibroblastos/metabolismo , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Transducción de Señal , Fracciones Subcelulares/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Xenopus/metabolismo

RESUMEN

Classical embryological experiments suggest that a posterior signal is required for patterning the developing anteroposterior axis. In this paper, we investigate a potential role for FGF signalling in this process. During normal development, embryonic fibroblast growth factor (eFGF) is expressed in the posterior of the Xenopus embryo. We have previously shown that overexpression of eFGF from the start of gastrulation results in a posteriorised phenotype of reduced head and enlarged proctodaeum. We have now determined the molecular basis of this phenotype and we propose a role for eFGF in normal anteroposterior patterning. In this study, we show that the overexpression of eFGF causes the up-regulation of a number of posteriorly expressed genes, and prominent among these are Xcad3, a caudal homologue, and the Hox genes, in particular HoxA7. There is both an increase of expression within the normal domains and an extension of expression towards the anterior. Application of eFGF-loaded beads to specific regions of gastrulae reveals that anterior truncations arise from an effect on the developing dorsal axis. Similar anterior truncations are caused by the dorsal overexpression of Xcad3 or HoxA7. This suggests that this aspect of the eFGF overexpression phenotype is caused by the ectopic activation of posterior genes in anterior regions. Further results using the dominant negative FGF receptor show that the normal expression of posterior Hox genes is dependent on FGF signalling and that this regulation is likely mediated by the activation of Xcad3. The biological activity of eFGF, together with its expression in the posterior of the embryo, make it a good candidate to fulfil the role of the 'transforming' activity proposed by Nieuwkoop in his 'activation and transformation' model for neural patterning.

Asunto(s)

Tipificación del Cuerpo/genética , Inducción Embrionaria/genética , Factores de Crecimiento de Fibroblastos/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas de Homeodominio/metabolismo , Animales , Proteínas de Drosophila , Ectodermo/metabolismo , Factores de Crecimiento de Fibroblastos/genética , Genes Homeobox , Proteínas de Homeodominio/genética , Hibridación in Situ , Mesodermo/metabolismo , Modelos Biológicos , Proteínas Recombinantes/biosíntesis , Distribución Tisular , Factores de Transcripción , Xenopus/embriología , Xenopus/genética

RESUMEN

Microsurgical, tissue grafting and in situ hybridization techniques have been used to investigate the role of the neural tube and notochord in the control of the myogenic bHLH genes, QmyoD, Qmyf5, Qmyogenin and the cardiac alpha-actin gene, during somite formation in stage 12 quail embryos. Our results reveal that signals from the axial neural tube/notochord complex control both the activation and the maintenance of expression of QmyoD and Qmyf5 in myotomal progenitor cells during the period immediately following somite formation and prior to myotome differentiation. QmyoD and Qmyf5 expression becomes independent of axial signals during myotome differentiation when somites activate expression of Qmyogenin and alpha-actin. Ablation studies reveal that the notochord controls QmyoD activation and the initiation of the transcriptional cascade of myogenic bHLH genes as epithelial somites condense from segmental plate mesoderm. The dorsal medial neural tube then contributes to the maintenance of myogenic bHLH gene expression in newly formed somites. Notochord grafts can activate ectopic QmyoD expression during somite formation, establishing that the notochord is a necessary and sufficient source of diffusible signals to initiate QmyoD expression. Myogenic bHLH gene expression is localized to dorsal medial cells of the somite by inhibitory signals produced by the lateral plate and ventral neural tube. Signaling models for the activation and maintenance of myogenic gene expression and the determination of myotomal muscle in somites are discussed.

Asunto(s)

Proteínas de Unión al ADN , Inducción Embrionaria , Secuencias Hélice-Asa-Hélice , Músculos/embriología , Factores Reguladores Miogénicos/biosíntesis , Notocorda/embriología , Transactivadores , Transcripción Genética , Animales , Trasplante de Células , Hibridación in Situ , Modelos Biológicos , Proteínas Musculares/biosíntesis , Proteínas Musculares/genética , Proteína MioD/biosíntesis , Proteína MioD/genética , Factor 5 Regulador Miogénico , Factores Reguladores Miogénicos/genética , Miogenina/biosíntesis , Miogenina/genética , Codorniz , Sondas ARN

RESUMEN

In recent years we and others have been attempting to identify the molecular nature of the inducing signals in early Xenopus development. We have found that most members of the fibroblast growth factor (FGF) family are biologically active as mesoderm-inducing factors when applied to ectoderm from blastulae. In addition to this, they will support continued expression of the pan-mesodermal transcription factor Xbra in the mesoderm of gastrula stage embryos. We have studied the expression pattern of four types of FGF in early embryos. Two types (FGF-2 and FGF-9) are expressed maternally and are thus present at the time of natural mesoderm induction. The expression of two other types (FGF-3 and FGF-4) is activated in the newly formed mesoderm of the gastrula. If the activity of the FGF family is inhibited by overexpression of a dominant-negative FGF receptor, there is a reduction in mesoderm formation, there are abnormalities arising from an inhibition of normal gastrulation movements and there is a defect in formation of the posterior parts. We believe that the mesoderm formation and cell movement effects are attributable to loss of Xbra expression, and the posterior defects to lack of posterior HOX gene activity. Overexpression of eFGF gives rise to a posteriorized phenotype, in which posterior HOX genes are expressed in a more anterior position. We conclude that the FGF system has multiple functions in early development, including mesoderm formation, gastrulation movements and anteroposterior patterning.

Asunto(s)

Factores de Crecimiento de Fibroblastos/fisiología , Xenopus/embriología , Animales , Blastocisto/fisiología , Factores de Crecimiento de Fibroblastos/genética , Gástrula/fisiología

RESUMEN

A detailed study of the expression pattern of embryonic fibroblast growth factor (eFGF) during early Xenopus development has been undertaken using whole-mount DIG in situ hybridization. We show that the zygotic expression of eFGF is activated in the mesoderm of the early gastrula and is first visualized as a ring around the blastopore, with significantly higher levels of expression on the dorsal side of the embryo. As gastrulation proceeds, eFGF transcripts become increasingly abundant in the dorsal blastopore lip. In the early neurula eFGF expression can be detected in the extreme posterior of the embryo around the closed blastopore and in the cells of the notochord. This latter result is significant and represents the first report of a Xenopus FGF that is expressed in the notochord. In addition, we show that during gastrula and neurula stages, expression of eFGF closely follows the expression of the Xenopus brachyury (Xbra) gene. During later development eFGF expression is localized to the tail-bud region and a stripe at the mid-brain/hind-brain junction. These data provide further evidence that FGFs play an important role in regulating the expression of brachyury in the developing mesoderm.

Asunto(s)

Embrión no Mamífero/fisiología , Factores de Crecimiento de Fibroblastos/biosíntesis , Expresión Génica , Proteínas de Dominio T Box , Xenopus laevis/embriología , Animales , Proteínas de Unión al ADN/biosíntesis , Embrión no Mamífero/citología , Proteínas Fetales/biosíntesis , Gástrula/fisiología , Hibridación in Situ , Cola (estructura animal)/embriología , Transcripción Genética

RESUMEN

We show that, in addition to a role in mesoderm induction during blastula stages, FGF signalling plays an important role in maintaining the properties of the mesoderm in the gastrula of Xenopus laevis. eFGF is a maternally expressed secreted Xenopus FGF with potent mesoderm-inducing activity. However, it is most highly expressed in the mesoderm during gastrulation, suggesting a role after the period of mesoderm induction. eFGF is inhibited by the dominant negative FGF receptor. Embryos overexpressing the dominant negative receptor show a change of behaviour of the dorsal mesoderm such that it moves around the blastopore lip instead of elongating in an antero-posterior direction. In such embryos there is a reduction in Xbra expression during gastrulation. We show that during blastula stages eFGF and Xbra are able to activate the expression of each other, suggesting that they are components of an autocatalytic regulatory loop. Moreover, we show that Xbra expression in isolated gastrula mesoderm cells is maintained by eFGF, suggesting that eFGF continues to regulate the expression of Xbra in the blastopore region. In addition, overexpression of eFGF after the mid-blastula transition results in the up-regulation of Xbra expression during gastrula stages and causes suppression of the head and enlargement of the proctodeum, which is the converse of the posterior reductions of the FGF dominant negative receptor phenotype. These data suggest an important role for eFGF in regulating the expression of Xbra and for the eFGF-Xbra regulatory pathway in the control of mesodermal cell behaviour during gastrula stages.

Asunto(s)

Factores de Crecimiento de Fibroblastos/fisiología , Regulación del Desarrollo de la Expresión Génica/fisiología , Factores de Transcripción/biosíntesis , Proteínas de Xenopus/fisiología , Xenopus laevis/embriología , Animales , Blastocisto/fisiología , Técnicas de Cultivo , Gástrula/fisiología , Mesodermo/fisiología , Fenotipo , Receptores de Factores de Crecimiento de Fibroblastos/fisiología , Xenopus laevis/genética , Xenopus laevis/metabolismo

Asunto(s)

Sistema Nervioso Central/embriología , Proteínas de Drosophila , Inducción Embrionaria/genética , Extremidades/embriología , Morfogénesis/genética , Proteínas/fisiología , Transactivadores , Animales , Embrión de Pollo , Pollos/genética , Drosophila melanogaster/embriología , Drosophila melanogaster/genética , Desarrollo Embrionario y Fetal/genética , Regulación de la Expresión Génica , Genes , Genes de Insecto , Proteínas Hedgehog , Ratones/embriología , Ratones/genética , Familia de Multigenes , Notocorda/fisiología , Ratas/embriología , Ratas/genética , Especificidad de la Especie , Factores de Transcripción/genética , Factores de Transcripción/fisiología , Pez Cebra/embriología , Pez Cebra/genética

RESUMEN

We report the cloning of two new quail myogenic cDNAs, quail myogenic factor 2 (qmf2) and qmf3, which encode helix-loop-helix proteins homologous to mammalian myogenic factors myogenin and myf-5. In situ hybridization has been used to investigate the developmental expression of qmf2 and qmf3, as well as qmf1, the quail homologue to mammalian MyoD1, during the formation of the brachial somites. These studies show that qmf1 and qmf3 are activated sequentially in medially localized somite cells, immediately following somite formation but prior to myotome formation. qmf1, qmf2, and qmf3 are expressed in the myotome of compartmentalized somites. These findings suggest that determination of the myogenic cell lineage in quail somites is a progressive process controlled by influences of the neural tube on the expression of the qmf regulatory genes in newly forming somites.

Asunto(s)

Coturnix/genética , Genes Reguladores , Proteínas Musculares/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , Coturnix/embriología , Inducción Embrionaria , Regulación de la Expresión Génica/genética , Datos de Secuencia Molecular , Morfogénesis , Músculos/embriología , Miogenina , Columna Vertebral/embriología