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1.
Mikrobiol Z ; 74(4): 71-9, 2012.
Artículo en Ruso | MEDLINE | ID: mdl-23088103

RESUMEN

Viral infections take the key place in medical practice. A large group of diseases are caused by adenoviral and herpes infection. As a rule, the investigations carried out in scientific laboratories are directed to the study of certain aspects of the interaction between the virus and the cell on the model of single infection. At the same time different viral infections at the level of macroorganism are constantly interacting with each other. The paper presents the studies on the selection of optimal models of lymphoblastoid cell lines for analysis of peculiarities of the mixed adeno-herpetic infection. The reproduction of human adenovirus type 5 and Epstein-Barr virus in the mono- and mixed infection in lymphoblastoid cell cultures of B-phenotype: B95-8, Raji, Namalwa have been studied. Both interfering and inhibiting action of viruses are shown. So EBV, which has a short 48-hour cycle of reproduction, inhibits the adenovirus at the given time. The obtained cell models of adeno-herpes infection will allow to study peculiarities of the antiviral action of etiotropic antiviral preparations in conditions of coinfection.


Asunto(s)
Adenovirus Humanos/crecimiento & desarrollo , Antígenos Virales/análisis , Linfocitos B/virología , ADN Viral/análisis , Herpesvirus Humano 4/crecimiento & desarrollo , Infecciones por Adenoviridae/inmunología , Infecciones por Adenoviridae/patología , Infecciones por Adenoviridae/virología , Adenovirus Humanos/inmunología , Antibiosis , Antígenos Virales/biosíntesis , Linfocitos B/inmunología , Técnicas de Cultivo de Célula , Línea Celular Tumoral , Proliferación Celular , Supervivencia Celular , Coinfección , Infecciones por Herpesviridae/inmunología , Infecciones por Herpesviridae/patología , Infecciones por Herpesviridae/virología , Herpesvirus Humano 4/inmunología , Humanos , Recuento de Linfocitos , Modelos Biológicos , Carga Viral , Replicación Viral
2.
Mikrobiol Z ; 67(3): 71-80, 2005.
Artículo en Ruso | MEDLINE | ID: mdl-16018208

RESUMEN

The paper deals with the influence of the adenovirus (Ad) and Epstein-Barr virus (EBV) on functional activity of lymphocytes, in particular, the production of alpha- and gamma-interferons, tumor necrosis factor (TNF) in conditions of mono- or double infection of B- and T-phenotype (CEM) lymphoblastoid cells. It is shown, that Ad, EBV or both viruses induce high enough levels of interferon on both lines of cells and in control epithelial cells. The lymphoblastoid cells infected by viruses deep ability to synthesize alpha- and gamma-interferons under the influence of the corresponding inducers (Newcastle disease virus and hemagglutinine). Nevertheless, the levels of their formation are not high. Rather high parameters of activity of the tumor necrosis factor (TNF) were revealed during a day in the initial B95-8 cells and superinfected Ad after the effect of LPS of E. coli. Their activity in CEM cells also did not depend on the infection type.


Asunto(s)
Infecciones por Adenovirus Humanos/inmunología , Linfocitos B/virología , Infecciones por Herpesviridae/inmunología , Inductores de Interferón , Linfocitos T/virología , Infecciones por Adenovirus Humanos/virología , Linfocitos B/inmunología , Células Cultivadas , Células Epiteliales/inmunología , Células Epiteliales/virología , Hemaglutininas , Infecciones por Herpesviridae/virología , Interferón-alfa/biosíntesis , Interferón gamma/biosíntesis , Virus de la Enfermedad de Newcastle , Linfocitos T/inmunología , Factor de Necrosis Tumoral alfa/biosíntesis
3.
Tsitol Genet ; 32(4): 82-8, 1998.
Artículo en Ruso | MEDLINE | ID: mdl-9813890

RESUMEN

Some indices have been studied which characterized the state of Epstein-Barr virus genome and adenovirus in the implanted lines of lymphoblastoid cells of B and T phenotype under the mixed or monoinfection. It has been shown that super infection by type 2 adenovirus rather sharply affects the state of Epstein-Barr virus genome in the Raji cells containing integrated Epstein-Barr virus genome. The state of adenovirus genome in the studied cells is less subject to changes. Its early area is revealed by hybridization using DNA-DNA method in a form of two fragments of different intensity which is maximum in the Raji and Jurkat cells, which evidences for the more expressivity of adenovirus genome in these cells.


Asunto(s)
Infecciones por Adenovirus Humanos/genética , Adenovirus Humanos/genética , Linfocitos B/virología , Genoma Viral , Infecciones por Herpesviridae/genética , Herpesvirus Humano 4/genética , Linfocitos T/virología , Infecciones Tumorales por Virus/genética , Infecciones por Adenovirus Humanos/virología , Animales , Callithrix , Línea Celular , Células Cultivadas , ADN Viral/genética , Infecciones por Herpesviridae/virología , Humanos , Hibridación de Ácido Nucleico/métodos , Reacción en Cadena de la Polimerasa/métodos , Infecciones Tumorales por Virus/virología , Cultivo de Virus
4.
Mikrobiol Z ; 59(6): 12-20, 1997.
Artículo en Ruso | MEDLINE | ID: mdl-9511371

RESUMEN

A comparative characteristic of the reproduction process of type 2 human adenovirus in several lines of lymphoblastoid cells of B- and T-phenotype is presented. Formation of hexone and infectious virus in the cells of Jurkat, MT4, Raji lines was rather intensive and approached to that in the culture of the permissive epithelium cells Hep-2. These indices were much lower in the cultures of cells B 95-8 and MT4/BIII LBK which were chronically infected by VEB and HIV, accordingly and produced them that can evidence for the interference of Ad and VEB or Ad and HIV under superinfection of cells. Cells of SEM line possessing T-phenotype, were apparently semi-permissive for Ad h2, though the low almost unchanged content of hexone and infectious virus remains in them for a rather long time: about 15 days. Thus, obtained data within analyzed series of experiments expand the present ideas about lymphotropicity of Ad as their important property realized at the level of cell and infected macroorganism.


Asunto(s)
Adenovirus Humanos/fisiología , Linfocitos B/virología , Proteínas de la Cápside , Linfocitos T/virología , Replicación Viral/fisiología , Adenovirus Humanos/genética , Adenovirus Humanos/inmunología , Animales , Antígenos Virales/análisis , Antígenos Virales/biosíntesis , Linfocitos B/inmunología , Cápside/análisis , Cápside/biosíntesis , Línea Celular , Células Cultivadas , Regulación Viral de la Expresión Génica/fisiología , Humanos , Linfocitos T/inmunología , Factores de Tiempo , Cultivo de Virus/métodos
5.
Mol Biol (Mosk) ; 23(4): 1022-35, 1989.
Artículo en Ruso | MEDLINE | ID: mdl-2685544

RESUMEN

Microinjection of either type 1 human adenovirus, type SA7 monkey adenovirus virions or circular adenovirus DNA, obtained by the treatment of DNA-terminal protein complexes with glutaraldehyde, into nuclei of permissive cells results in the complete cycle of virus reproduction. Microinjection of neither linear native, condensed adenovirus DNA nor the DNA-terminal protein complexes under the same conditions initiates the adenovirus reproduction thought the synthesis of early and some late viral antigens is observed in the injected cells. Integration of injected adenovirus DNA into the cellular DNA occurs as far as 30 min after injection. Microinjection of either adenovirus DNA or its oncogene containing fragments into nuclei of semipermissive cells induces the transformation of these cells. In this case the time of the first appearance of transformation foci is decreased.


Asunto(s)
Adenoviridae/genética , ADN Recombinante/genética , ADN Viral/genética , Transfección , Virión/genética , Animales , Autorradiografía , Transformación Celular Neoplásica/genética , Transformación Celular Viral/genética , Células Cultivadas , ADN Viral/ultraestructura , Electroforesis en Gel de Agar , Técnica del Anticuerpo Fluorescente , Humanos , Microinyecciones , Hibridación de Ácido Nucleico , Plásmidos , Timidina Quinasa/genética , Proteínas Virales/metabolismo
6.
Mol Biol (Mosk) ; 17(5): 972-5, 1983.
Artículo en Ruso | MEDLINE | ID: mdl-6314121

RESUMEN

EV40 DNA closed superhelical form was introduced into cell nuclei by means of direct microinjection and expression of the viral genome followed. The expression of viral T-antigen was observed 40 hours after the injection, and SV40-specific effect developed on the fifth day. Using the method discussed, the infection dose of SV40 DNA in a permissive cell culture system was shown to be approximately one molecule per cell nucleus.


Asunto(s)
Transformación Celular Viral , ADN Viral/genética , Genes Virales , Virus 40 de los Simios/genética , Animales , Antígenos Virales de Tumores/genética , Línea Celular , Núcleo Celular/metabolismo , ADN Superhelicoidal/genética , Haplorrinos , Cinética , Microinyecciones , Virus 40 de los Simios/inmunología
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