1.
Spectrochim Acta A Mol Biomol Spectrosc
; 222: 117163, 2019 Nov 05.
Artículo
en Inglés
| MEDLINE
| ID: mdl-31177008
RESUMEN
A linear spectral mapping technique was applied to monitor the growth of biomolecular absorption bands at the bio-interface of a nascent Pseudomonas fluorescens biofilm during and after interaction with a surface-adhered air bubble. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectra were obtained in different locations in a microchannel with adequate spatial and temporal resolution to study the effect of a static bubble on the evolution of protein and lipid signals at the ATR crystal surface. The results reveal that the presence of a bubble during the lag phase modified levels of extracellular lipids and affected a surface restructuring process, many hours after the bubble's disappearance.