RESUMEN

Hg and Cd are the two most toxic heavy metal ions that could be found in aqueous solutions. In this study, a chemosensor based on 5-(4-((4-nitrophenyl) diazenyl) phenyl)-1,3,4-oxadiazole-2-thiol (DOT) was reported to detect these ions simultaneously. DOT showed high selectivity towards Hg ion by changing the color of the solution from beige to gold-yellow at different concentrations of Hg ion. In comparison, other relevant metals, such as Li+, Na+, K+, Cs+, Mg2+, Ca2+, Al3+, Fe2+, Ag+, Cu2+, Pb2+, Ni2+, Zn2+, Cr3+, Fe3+, Pb4+, Mn2+, and Cd2+ did not affect the color of the DOT solution as the interfering ions. Despite no changes in the color of DOT solution in the presence of Cd ion, a solution containing DOT-Hg complex was changed from gold-yellow to orange by adding Cd ion, providing an approach for detecting Hg and Cd ion simultaneously with UV-Vis and Fluorescent spectroscopy. DOT exhibited a high association constant with a detection limit of 0.05 µM for Hg and Cd ions in an aqueous solution. The results of quantum mechanics (QM) calculations were also consistent with the experimental observations, which indicated that changes in the band gap could explain the various colors of DOT complex with metal ions.

RESUMEN

Interpenetrated network (IPN) hydrogels with desired mechanical properties were prepared based on gelatin. A copolymer of dimethyl aminoethyl methacrylate (DMAEMA) with 2-Acrylamido-2-methylpropane sulfonic acid (AMPS) in gelatin was chemically cross-linked with methylene bis acrylamide (MBA) to form a semi-IPN hydrogel. Also, IPN hydrogel is fabricated from the AMPS-co-DMAEMA and gelatin in the presence of ferric ions with both chemical and physical cross-linkers. According to the compression test, the metal-ligand interaction has a remarkable impact on the mechanical strength of hydrogel. Ferric ions caused a decrease in the pores size confirmed by the SEM images of hydrogels, resulting in preserving its mechanical stability during the swelling test due to a more robust structure of hydrogel. Ferric to ferrous ions reduction is observed under visible light irradiation, which results in a light-sensitive hydrogel with a higher rate of biodegradation compared to semi-IPN hydrogels. MTT assay results implied that the synthesized hydrogels are non-toxic for the L-929 cell line. Also, for more detailed investigations, histological studies are conducted as in vivo tests. With regards to the improvements of mechanical properties harnessed in IPN hydrogels by ferric ions along with the extraordinary self-healing capability, IPNs would be considered an appropriate option for tissue engineering.

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RESUMEN

Injectable stimuli-responsive hydrogels could offer an opportunity for local administration at the tumor site and a sustained drug release. In this paper, a copolymer of azobenzene derivative and N-isopropyl acrylamide (NIPAM) was synthesized, which are performed as light- and thermo-sensitive parts, respectively. The DAS@SCD/NIPAZO hydrogel was prepared upon the establishment of host-guest interactions between the hydrophobic core of CD and azobenzene moiety. The LCST of the synthesized copolymer was modified from 31.3 °C to 36.5 °C by the incorporation of the hydrophilic host moieties of the modified starch into the NIPAM copolymer structure. The LCST-based property of the hydrogel made it syringable in low temperatures and switch to a gel state after local injection. The drug release profile of the hydrogel was explored in four different conditions involving two distinct temperatures combined with two different light wavelengths to examine the light- and thermo-sensitivity of the hydrogel. Moreover, a Paclitaxel-loaded hydrogel was prepared to study the in vitro efficiency of the sample and was investigated by MTT assay against the cancerous fibroblastic cells (A-431), which revealed a sharp decline in cell viability under 365 nm light irradiation; furthermore, to evaluate the in vivo effects of the PTX-loaded hydrogel, histological studies based on staining techniques were carried out.

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