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ABSTRACT: Mylott, JA, Potts, EM, Wolf, JH, Bullock, GS, and Nicholson, KF. Kinematic and kinetic differences between ball rotational exercises and the throwing motion in collegiate baseball athletes. J Strength Cond Res XX(X): 000-000, 2024-The purpose of this study was to observe the associations that medicine ball pushes and chops have on the lower extremities regarding ground reaction forces (GRF) and lead knee flexion angles and to compare these values with a regular baseball overhead throw. Lower extremity kinetics and kinematics were obtained via force plates and marker motion capture. Subjects (n = 35) included baseball players (age 19.7 ± 1.5 years). Five medicine ball movements of each type along with at least 3 pitches or 5 throws off the mound were recorded for data collection. Statistical parametric mapping, including analysis of variance and 2-way t-tests, was used to compare the variables of interest between the movements for continuous time data. All kinematic and kinetic variables were significantly different (p < 0.05) for some time during the motion between the different trial types of medicine ball exercises and throws. A medicine ball chop can increase peak drive leg GRF, whereas a push can help an athlete keep greater driver leg GRF and maintain ground connection into foot plant. A push helps train lead leg deficiencies in the delivery. Both the medicine ball chop and push have benefits for training lead leg stabilization and extension.
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Immunohistochemistry is a widely used technique to visualize specific tissue structures as well as protein expression and localization. Two alternative approaches are widely used to handle the tissue sections during the staining procedure, one approach consists of mounting the sections directly on glass slides, while a second approach, the free-floating, allows for fixed sections to be maintained and stained while suspended in solution. Although slide-mounted and free-floating approaches may yield similar results, the free-floating technique allows for better antibody penetration and thus should be the method of choice when thicker sections are to be used for 3D reconstruction of the tissues, for example when the focus of the experiment is to gain information on dendritic and axonal projections in brain regions. In addition, since the sections are kept in solution, a single aliquot can easily accommodate 30 to 40 sections, handling of which is less laborious, particularly in large-scale biomedical studies. Here, we illustrate how to apply the free-floating method to fluorescent immunohistochemistry staining, with a major focus on brain sections. We will also discuss how the free-floating technique can easily be modified to fit the individual needs of researchers and adapted to other tissues as well as other histochemical-based stainings, such as hematoxylin and eosin and cresyl violet, as long as tissue samples are properly fixed, typically with paraformaldehyde or formalin.