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Abstract This study reports the first assessment of endophytic fungi isolated from strawberry leaves and selection of isolates for the control of Duponchelia fovealis, a new pest of strawberries. A total of 400 strawberry leaves of the cultivar 'Albion' were collected in four commercial farms. Leaves were disinfected, cut in fragments, and placed on Petri dishes containing potato dextrose agar media with tetracycline and incubated for 30 days. Following this time, 517 fungal colonies were isolated, and thirteen genera were identified: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe, and Phoma. Eight isolates belonging to the genera Aspergillus, Diaporthe, Paecilomyces, and Cladosporium were selected for pathogenicity bioassays against third instar larvae of D. fovealis. Isolates of Paecilomyces induced the highest mortality rates.

Resumo Este trabalho apresenta a primeira contribuição no isolamento de fungos endofíticos de folhas de morangueiro e na seleção de isolados para controle de Duponchelia fovealis, uma nova praga do morangueiro. Foram coletadas 400 folhas da cultivar 'Albion' em quatro lavouras comerciais de morangueiro. As folhas foram desinfetadas, cortadas em fragmentos e depositadas em placas de Petri contendo Ágar Batata Dextrose como meio, em conjunto com tetraciclina e incubados durante 30 dias. Um total de 517 colônias fúngicas e treze gêneros foram isolados: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe e Phoma. Oito isolados pertencentes aos gêneros Aspergillus, Diaporthe, Paecilomyces e Cladosporium foram selecionados para os bioensaios de virulência contra larvas de 3º instar de D. fovealis. Isolados de Paecilomyces causaram as maiores taxas de mortalidade.

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This study reports the first assessment of endophytic fungi isolated from strawberry leaves and selection of isolates for the control of Duponchelia fovealis, a new pest of strawberries. A total of 400 strawberry leaves of the cultivar 'Albion' were collected in four commercial farms. Leaves were disinfected, cut in fragments, and placed on Petri dishes containing potato dextrose agar media with tetracycline and incubated for 30 days. Following this time, 517 fungal colonies were isolated, and thirteen genera were identified: Cladosporium, Aspergillus, Nigrospora, Fusarium, Trichoderma, Chaetomium, Alternaria, Paecilomyces, Penicillium, Ulocladium, Bipolaris, Diaporthe, and Phoma. Eight isolates belonging to the genera Aspergillus, Diaporthe, Paecilomyces, and Cladosporium were selected for pathogenicity bioassays against third instar larvae of D. fovealis. Isolates of Paecilomyces induced the highest mortality rates.

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Aphids are sap-sucking insects that mainly attack shoots and young leaves of peach trees and many other plant species; however, knowledge of the Brazilian aphid fauna is scant. The objective of this study was to identify aphid species collected in peach orchards (Prunus persica Batsch) and to determine their faunal indices for occurrence and dominance. The experiment was conducted from July 2005 to September 2006 in six Chimarrita peach orchards in the municipality of Araucária, PR, Brazil. The survey of aphid species was conducted by visual samplings on peach trees and using Möericke-type yellow traps containing water. A faunal analysis was made using aphid occurrence and dominance indices. Brachycaudus persicae (Passerini, 1860) was the only aphid species that was found colonizing peach in Araucária/PR. Although most aphids collected were classified as rare, some can be considered potential peach colonizers, such as Myzus persicae (Sulzer, 1776) which was given the status of common or intermediate in some of the orchards studied. The population fluctuation of aphids showed a negative correlation with rainfall and positive correlation with temperature and relative humidity.

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Aphids are sap-sucking insects that mainly attack shoots and young leaves of peach trees and many other plant species; however, knowledge of the Brazilian aphid fauna is scant. The objective of this study was to identify aphid species collected in peach orchards (Prunus persica Batsch) and to determine their faunal indices for occurrence and dominance. The experiment was conducted from July 2005 to September 2006 in six Chimarrita peach orchards in the municipality of Araucária, PR, Brazil. The survey of aphid species was conducted by visual samplings on peach trees and using Mõericke-type yellow traps containing water. A faunal analysis was made using aphid occurrence and dominance indices. Brachycaudus persicae (Passerini, 1860) was the only aphid species that was found colonizing peach in Araucária/PR. Although most aphids collected were classified as rare, some can be considered potential peach colonizers, such as Myzus persicae (Sulzer, 1776) which was given the status of common or intermediate in some of the orchards studied. The population fluctuation of aphids showed a negative correlation with rainfall and positive correlation with temperature and relative humidity.

Os afídeos são insetos sugadores de seiva que atacam principalmente brotações e folhas novas de pessegueiros e de diversas outras espécies vegetais, porém o conhecimento sobre a afidofauna brasileira é escasso. O objetivo deste trabalho foi identificar as espécies de afídeos coletadas em pomares de pessegueiros (Prunus persica Batsch) e analisar os índices faunísticos de ocorrência e dominância. O experimento foi realizado de julho de 2005 a setembro de 2006 em seis pomares de pessegueiros da cultivar Chimarrita no município de Araucária, PR. O levantamento das espécies de afídeos foi realizado por meio de amostragens visuais em pessegueiros e armadilhas amarelas de água do tipo Mõericke. Brachycaudus persicae (Passerini, 1860) foi a única espécie de afídeo encontrada colonizando pessegueiros em Araucária, PR. Apesar da maioria das espécies de afídeos coletadas serem classificadas como raras, algumas podem ser consideradas colonizadoras potenciais de pessegueiros como Myzus persicae (Sulzer, 1776) que recebeu o status de comum ou intermediária em alguns dos pomares estudados. A flutuação populacional de afídeos apresentou uma correlação negativa com a precipitação pluviométrica e positiva com a temperatura e a umidade relativa do ar.

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