Asunto(s)
Mitocondrias/fisiología , Animales , Línea Celular , Metabolismo Energético , Colorantes Fluorescentes , Membranas Intracelulares/metabolismo , Membranas Intracelulares/fisiología , Potenciales de la Membrana , Mitocondrias/metabolismo , Mitocondrias/ultraestructura , Rodamina 123 , Rodaminas , PorcinosRESUMEN
Plasminogen activator has been isolated from the culture of pig kidney cells by gel-filtration on Sephadex G-75. The plasminogen activator (181.9 MU/200 g body weight) protected the animals against thrombosis hazard in case of provocation by thromboplastin and exerted a thrombolytic effect in case of thrombus appearance.
Asunto(s)
Activadores Plasminogénicos/farmacología , Animales , Línea Celular , Células Cultivadas , Evaluación Preclínica de Medicamentos , Fibrinólisis/efectos de los fármacos , Venas Yugulares , Riñón , Masculino , Activadores Plasminogénicos/aislamiento & purificación , Activadores Plasminogénicos/uso terapéutico , Ratas , Porcinos , Terapia Trombolítica , Trombosis/tratamiento farmacológicoRESUMEN
Twenty-five patients with type I diabetes mellitus (8 women and 7 men) aged 19 to 54 with a disease duration of 3 to 19 years were treated by Russian semisynthetic short-acting human insulin mono-H. The daily dose varied from 24 to 44 U. The drug therapeutic efficacy was assessed from the terms of attainment of carbohydrate metabolism compensation, glycosylated hemoglobin level, and changes in daily insulin requirement. Clinical safety of the drug was assessed from changes in antiinsulin antibody level, incidence and severity of hypoglycemia, absence of lipodystrophy and other adverse reactions. The drug was found effective for the treatment of patients with type I diabetes. Its hypoglycemic effect was similar to that of actrapid, a porcine insulin. Fractionated insulin therapy resulted in the majority of patients in a reduction of glycosylated hemoglobin level, of daily requirement in insulin, and of antiinsulin antibody titer.
Asunto(s)
Diabetes Mellitus Tipo 1/tratamiento farmacológico , Insulina/uso terapéutico , Adulto , Anticuerpos Monoclonales/sangre , Femenino , Humanos , Hipoglucemia/inducido químicamente , Hipoglucemia/epidemiología , Incidencia , Insulina/efectos adversos , Insulina/inmunología , Masculino , Persona de Mediana EdadRESUMEN
Stable compensation of diabetes mellitus, including normolipidemia, underlies the therapy of diabetic angiopathies. Mevacor represents a nonactive lactone form of a certain hydroxy acid, a potent inhibitor of endogenous synthesis of cholesterol, conducive to blood cholesterol reduction. The aim of the present study was the assessment of the efficacy of this drug in therapy of patients with diabetes mellitus. Ten patients were administered mevacor in a dose of 20 mg for a month. Such therapy was conducive to a significant reduction of the levels of cholesterol, LNP cholesterol, triglycerides, and cholesterol/LVP ratio. It also promoted a reduction of the content of lipid peroxidation products in the blood, these products being an active factor of vessel destruction. The levels of hydroperoxides, blood serum and red cell malonic dialdehyde, and superoxide dismutase were also reduced. These results necessitate addition of mevacor to a complex of therapy for diabetes to normalize lipid metabolism.
Asunto(s)
Diabetes Mellitus Tipo 2/tratamiento farmacológico , Angiopatías Diabéticas/tratamiento farmacológico , Lovastatina/uso terapéutico , Adulto , Anciano , Colesterol/sangre , LDL-Colesterol/sangre , Diabetes Mellitus Tipo 2/sangre , Angiopatías Diabéticas/sangre , Humanos , Persona de Mediana Edad , Triglicéridos/sangreRESUMEN
Two artificial genes, encoding two forms of human granulocyte colony stimulating factor as products of a normal and an alternative splicing, have been by a chemical-enzymatic way synthesized and cloned in Escherichia coli. The genes are supplied with recognition sites of restriction endonucleases to facilitate the further cassette mutagenesis.
Asunto(s)
Escherichia coli/genética , Genes Bacterianos , Genes Sintéticos , Factor Estimulante de Colonias de Granulocitos/genética , Secuencia de Aminoácidos , Autorradiografía , Secuencia de Bases , Clonación Molecular , ADN , Enzimas de Restricción del ADN , Electroforesis en Gel de Poliacrilamida , Humanos , Datos de Secuencia Molecular , Plásmidos , Empalme del ARNAsunto(s)
Riñón/efectos de los fármacos , Oligomicinas/farmacología , Consumo de Oxígeno/efectos de los fármacos , Animales , Células Cultivadas/efectos de los fármacos , Células Cultivadas/metabolismo , Concentración de Iones de Hidrógeno , Riñón/metabolismo , Microscopía Fluorescente , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Polarografía , Factores de TiempoRESUMEN
It is shown that 2,4-dinitrophenol and dicyclohexylcarbodiimide, energy metabolism inhibitors, induce regular specific changes of the HeLa culture cells ultrastructure. Peculiarities of metabolism of the tumor cells as against the normal culture cells cause weaker and slower response.
Asunto(s)
Carbodiimidas/farmacología , Diciclohexilcarbodiimida/farmacología , Dinitrofenoles/farmacología , Metabolismo Energético/efectos de los fármacos , Células Tumorales Cultivadas/efectos de los fármacos , Desacopladores/farmacología , 2,4-Dinitrofenol , Animales , Células HeLa , Humanos , Porcinos , Células Tumorales Cultivadas/ultraestructuraRESUMEN
Formation of virus-specific RNAs in the presence of bonafton, actinomycin D, and cycloheximide was studied. The degree of bonafton inhibition of the synthesis of the genome and complementary RNAs in the nucleus and cytoplasm of the infected cells varied. The effect of bonafton on replication and transcription of influenza virus differs from that of actinomycin and cycloheximide. Bonafton appears to disturb the regulation of these processes, specifically inhibiting the synthesis of individual fragments of viral RNA.
Asunto(s)
Antivirales/farmacología , Virus de la Influenza A/efectos de los fármacos , Naftoquinonas/farmacología , Replicación Viral/efectos de los fármacos , Animales , Embrión de Pollo , Cicloheximida/farmacología , Dactinomicina/farmacología , Depresión Química , Genes Virales/efectos de los fármacos , Virus de la Influenza A/fisiología , Sustancias Macromoleculares , ARN Viral/antagonistas & inhibidoresRESUMEN
A simple procedure for measuring the cells respiration without their transformation into the suspension, is suggested: seven glass slides with the cell culture of PKEV (pork, kidney, embryo, versen) are placed into the polarographic cell. Under these conditions the oxygen electrode easily records the fall of oxygen tension in the polarographic cell due to the respiration of 10(5) cells. Oxygen consumption is linear in time at the O2 concentration within the range from 480 to 200 nanoatoms per 1 ml of medium. The respiration rate is found to be enhanced by uncoupler and suppressed by the inhibitors of the electron flow chain (NaCN, antimycin A, amytal, rotenone) and mitochondrial ATPase (oligomycin and diciclohexylcarbodiimide). The above approach enables to evaluate the mitochondrial respiration of any cell monolayer keeping the cells intact.