RESUMEN
1. Sex-related differences of long pelvic limb bones and serum bone metabolism indices were evaluated in 14-month-old female (N = 7) and male (N = 7) ostriches of similar body weights. 2. Densitometric parameters of femur, tibia and tarsometatarsus were determined using quantitative computed tomography (volumetric bone mineral density, calcium hydroxyapatite density and mean volumetric bone mineral density) and dual energy X-ray absorptiometry (bone mineral density and bone mineral content) methods. Geometrical parameters such as cortical bone area, cross-sectional area, second moment of inertia, mean relative wall thickness and cortical index were determined in the midshaft of bones. Mechanical properties of bones (maximum elastic strength and ultimate strength) were evaluated using three-point bending test. Serum concentrations of free amino acids, osteocalcin, N-terminal propeptide of type I procollagen, C-terminal telopeptides of type II collagen and total antioxidative capacity were also determined. 3. Bone weight and relative bone weight of all bones were significantly higher in males than in females. Significantly lower values of trabecular bone mineral density and calcium hydroxyapatite density were found in the trabecular bone of tibia in males. The highest number of the sex-related differences was observed in the tarsometatarsus where bone length, bone mineral content, cortical bone area, cross-sectional area and ultimate strength were higher in males. Serum concentrations of taurine, hydroxyproline, valine and isoleucine were significantly higher in males. 4. Higher loading of the tarsometatarsus in comparison to femur and tibia may be an important factor interacting with sex hormones in regulation of bone formation and mineralisation processes. Sex-related differences of bone properties were associated with increased serum concentration of selected amino acids in males.
Asunto(s)
Densidad Ósea , Huesos/metabolismo , Miembro Posterior/anatomía & histología , Struthioniformes/anatomía & histología , Struthioniformes/fisiología , Aminoácidos/metabolismo , Animales , Fenómenos Biomecánicos , Huesos/química , Densitometría/veterinaria , Femenino , Fémur/fisiología , Miembro Posterior/fisiología , Masculino , Valores de Referencia , Factores Sexuales , Tibia/fisiologíaRESUMEN
The aim of the current study was to investigate basic morphometric, geometric, and densitometric parameters of tibia and tarsometatarsus in 14-mo-old male and female ostriches, and interrelationships between these parameters. The study was conducted on 20 tibiae and 20 tarsometatarsal bones of the left pelvic limb derived from 20 healthy 14-mo-old ostriches (Struthio camelus): 10 males and 10 females. The following parameters were determined using peripheral quantitative computed tomography: bone mineral content (BMC), volumetric bone mineral density (vBMD), cortical content (CRT_CNT), cortical density (CRT_DEN), trabecular content, trabecular density (TRAB_DEN), bone area (TOT_A), trabecular area (TRAB_A), cortical area (CRT_A), cortical thickness (CRT_THK_C), periosteal circumference (PERI_C), endocortical circumference (ENDO_C), and strength-strain index (SSI) in the metaphysis and diaphysis of the bones. Statistical evaluation of the obtained results was performed using Student's t-test, and the Pearson correlation coefficient between the investigated parameters was determined. The obtained results have shown significant differences in proximal metaphysis between males and females when evaluating such parameters as CRT_DEN, TOT_A, TRAB_A, PERI_C, SSI in tibia (P < 0.05), and BMC, vBMD, TRAB_DEN, CRT_CNT, TOT_A, TRAB_A, cortical area, PERI_C, ENDO_C, SSI in tarsometatarsus (P < 0.05). Significant differences between the 2 sexes were found in all the investigated parameters of diaphysis of tibia, except for CRT_DEN and ENDO_C (P < 0.05). Significant positive correlations between BW and bone weight were found (P < 0.05). Furthermore, numerous correlations of morphometric, geometric, and densitometric parameters of metaphysis and diaphysis of the tibia and tarsometatarsus were stated (P < 0.05). In conclusion, the present investigation demonstrated sex-related differences in morphometric, densitometric, and geometric properties of tibia and tarsometatarsus in 14-mo-old ostriches. Numerous correleations observed between the investigated parameters have shown that ostrich tibia and tarsometatarsus may present a valuable model for further studies on bone tissue metabolism regulation in breeding birds.
Asunto(s)
Miembro Posterior/anatomía & histología , Miembro Posterior/fisiología , Struthioniformes/anatomía & histología , Struthioniformes/fisiología , Animales , Densidad Ósea , Femenino , Miembro Posterior/diagnóstico por imagen , Masculino , Huesos Metatarsianos/anatomía & histología , Huesos Metatarsianos/diagnóstico por imagen , Huesos Metatarsianos/fisiología , Caracteres Sexuales , Huesos Tarsianos/anatomía & histología , Huesos Tarsianos/diagnóstico por imagen , Huesos Tarsianos/fisiología , Tibia/anatomía & histología , Tibia/diagnóstico por imagen , Tibia/fisiología , Tomografía Computarizada por Rayos X/veterinariaRESUMEN
Pigs of Belgian Landrace (B), Duroc (D), Hampshire (H), and Pietrain (P) breeds were slaughtered at 90, 110 and 130 kg body weight (BW) and the carcass chemical composition and fatty acid profiles of intramuscular fat of loin and backfat were determined. The carcass of Pietrain pigs had a higher concentration of protein and less fat than of B, D and H breeds. In tissues, the PUFA:SFA ratio was lower in the heavier (130 kg BW) than in lighter pigs (90 and 110 kg BW). This feature was higher in P pigs compared with the other breeds. The backfat had a higher concentration of PUFA:SFA ratio than intramuscular fat. The PUFA n- 6:n- 3 ratio was not affected by the breed and weight of pigs, but it was lower in backfat than in longissimus muscle fat. The protein concentration in the carcass was positively related to PUFA and negatively to SFA concentration in tissues.