Asunto(s)
Computadores/legislación & jurisprudencia , Departamentos de Hospitales/legislación & jurisprudencia , Registros de Hospitales/legislación & jurisprudencia , Sistemas de Registros Médicos Computarizados/legislación & jurisprudencia , Checoslovaquia , Bases de Datos Factuales/legislación & jurisprudenciaAsunto(s)
Criocirugía , Enfermedades del Cuello del Útero/cirugía , Adolescente , Adulto , Femenino , Humanos , Persona de Mediana EdadRESUMEN
Dinitrophenyl-specific T-cell lines were established by culturing lymph node-derived lymphocytes from dinitrochlorobenzene-sensitized guinea-pigs with dinitrophenyl-modified macrophages. Cells from this line were all Ia-positive and formed E-rosettes with rabbit erythrocytes; no Ig-positive cells were present in this suspension. Expanded cells exhibited enhanced in vitro and in vivo activity as demonstrated by DNA synthesis and systemic adoptive transfer of contact sensitivity. In vitro DNA synthesis was elicited not only be specific hapten-modified macrophages but also by conjugates of the hapten with homologous (GPA) and even heterologous (BGG, HSA) proteins, thus demonstrating the loss of carrier specificity. Moreover, the proliferative response of expanded cells was elicited not only with hapten-modified syngeneic (strain 2) but also with allogeneic (strain 13) macrophages. Cells from hapten-modified T-cell lines, but not from lymph nodes from in vivo primed guinea-pigs, showed specific accumulation in contact sensitivity skin test sites. Attempts to establish hapten-specific T-cell clones were also at least partially successful.
Asunto(s)
Proteínas Portadoras/inmunología , Dermatitis por Contacto/inmunología , Haptenos/inmunología , Linfocitos T/inmunología , Animales , Línea Celular , Movimiento Celular , Células Clonales/inmunología , Dinitrofenoles/inmunología , Epítopos/inmunología , Cobayas , Inmunización Pasiva , Activación de Linfocitos , Piel/inmunologíaAsunto(s)
Dermatitis por Contacto/inmunología , Epítopos/inmunología , Haptenos/inmunología , Linfocitos T/inmunología , Animales , Movimiento Celular , Células Cultivadas , Dermatitis por Contacto/fisiopatología , Dinitroclorobenceno/inmunología , Femenino , Cobayas , Ganglios Linfáticos/citología , Masculino , Linfocitos T/fisiologíaRESUMEN
The antigen-presenting capability of macrophage modified with a single hapten (dinitrofluorobenzene) is partially or totally abolished by an additional haptenation with a second related (picryl chloride) or unrelated (oxazolone) hapten. This effect, 'antigenic competition', is only partially mediated by suppressor cells. There also seems to be an inhibition of the association of the hapten with particular Ia antigens. The prerequisite for antigenic competition is that both hapten responses are controlled by the same immune response gene. Hapten responses which are controlled by different genes, e.g., dinitrofluorobenzene, picryl chloride, and oxazolone on the one hand and benzilidene acetone on the other, do not compete. The pattern of competition thus varies with the strain of guinea pig.
Asunto(s)
Células Presentadoras de Antígenos/inmunología , Dermatitis por Contacto/inmunología , Macrófagos/inmunología , Animales , Unión Competitiva/efectos de los fármacos , Butanonas/inmunología , Ciclofosfamida/farmacología , Dinitroclorobenceno/inmunología , Dinitrofluorobenceno/inmunología , Femenino , Adyuvante de Freund , Genes MHC Clase II , Cobayas , Masculino , Oxazolona/inmunología , Cloruro de Picrilo/inmunología , Ácido Trinitrobencenosulfónico/inmunologíaRESUMEN
Using guinea pigs an analysis could be made of various aspects of contact sensitivity (CS) induced by subcutaneous injection of syngeneic haptenized macrophages (oil-induced peritoneal exudate cells, PEC) as compared to epicutaneous sensitization. Very little PEC-bound hapten (dinitrochlorobenzene, or oxazolone) is needed for optimum sensitization. Nevertheless, both sensitization methods induce a state of CS that may last for over 6 months, give rise to hapten-specific antibodies with a similar isotype distribution, and show susceptibility to cyclophosphamide pretreatment. In addition, time courses and microscopic appearance of skin test reactions after either way of sensitization are identical. CS to a broad variety of physicochemically different antigens, including nickel, penicillin, and acrylates, is readily induced by syngeneic PEC, haptenized following a standardized procedure. As Freund's complete adjuvant is known to cause serious side effects like ulceration and long-lasting granuloma formation, immunization with haptenized PEC should now be considered as a clean and effective alternative in experimental CS studies.
Asunto(s)
Alérgenos/inmunología , Dermatitis por Contacto/etiología , Haptenos/inmunología , Macrófagos/inmunología , Animales , Anticuerpos/análisis , Especificidad de Anticuerpos , Líquido Ascítico/inmunología , Dermatitis por Contacto/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Cobayas , Inmunización/métodos , Masculino , Pruebas Cutáneas , Factores de TiempoAsunto(s)
Trastornos Psicóticos/diagnóstico , Adulto , Errores Diagnósticos , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Guinea pig lymph node cells stimulated with concanavalin A (Con-A) were used as a source of lymphokines. Purifications by molecular sizing columns yielded sufficient quantities of macrophage activating factor (MAF)-active fractions to allow immunization of rabbits. An antiserum was produced which is capable of specifically inhibiting MAF activity, as well as blocking the activity of macrophage migration inhibiting factor (MIF), but not of skin reactive factor. Antisera raised against control supernatants derived from lymph node cells not stimulated with Con-A, failed to specifically inhibit any of these lymphokines. Additionally, anti-MAF but not anti-control antibodies suppressed completely contact sensitivity to dinitrochlorobenzene.
Asunto(s)
Sueros Inmunes , Linfocinas/inmunología , Animales , Formación de Anticuerpos , Cobayas , Inmunoquímica , Técnicas In Vitro , Linfocinas/aislamiento & purificación , Factores Inhibidores de la Migración de Macrófagos/inmunología , Factores Activadores de MacrófagosRESUMEN
in the present paper it is shown that both induction and elicitation of 2,4-dinitrochlorobenzene-contact sensitivity in guinea pigs are genetically controlled. This genetic control is operating by two somehow different mechanisms. The recognition of hapten on allogeneic macrophages during the inductive phase is suppressed by the allogeneic response against these macrophages whereas the elicitation upon transfer of syngeneically primed lymphocytes is limited by the identity of at least some components of the major histocompatibility complex. This nonidentity contradicts the rule of carrier specificity, which is a requirement for all delayed type hypersensitivity reactions. Furthermore, the presentation of haptens to primed cells is less restricted than presentation of protein antigens.
Asunto(s)
Proteínas Portadoras/inmunología , Dermatitis por Contacto/genética , Dinitroclorobenceno/inmunología , Epítopos/genética , Nitrobencenos/inmunología , Animales , Proteínas Portadoras/administración & dosificación , Membrana Celular/inmunología , Dermatitis por Contacto/etiología , Dermatitis por Contacto/inmunología , Dinitroclorobenceno/administración & dosificación , Femenino , Cobayas , Haptenos/administración & dosificación , Inmunización Pasiva , Macrófagos/inmunología , Masculino , Especificidad de la Especie , Linfocitos T Reguladores/inmunologíaRESUMEN
Expanded DNP-specific guinea pig T cells exhibit enhanced in vitro and in vivo activity as demonstrated by DNA synthesis and systemic adoptive transfer of contact sensitivity. Moreover, expanded lymphocytes are capable of accomplishing local passive transfer of contact sensitivity and producing interleukin 2, vascular permeability-increasing and macrophage migration-inhibiting factors. Expansion of hapten-specific lymphocytes offers a suitable model for studying eliciting mechanism of allergic contact dermatitis in humans.
Asunto(s)
Dermatitis por Contacto/fisiopatología , Linfocitos T/fisiología , Animales , Células Cultivadas , ADN/biosíntesis , Dermatitis por Contacto/inmunología , Dinitroclorobenceno , Femenino , Cobayas , Haptenos/inmunología , Inmunización Pasiva , Interleucina-2/biosíntesis , Masculino , Linfocitos T/metabolismoRESUMEN
Guinea pigs sensitized with either the trivalent chromium chloride or the hexavalent potassium dichromate are capable of reacting in vivo and in vitro to challenges with both chromium salts. This double reactivity is retained also after repeated restimulations with only 1 of these chromium compounds. From the failure to select lymphocytes directed specifically against a chromium determinant of a particular valence it is concluded that by sensitization with chromium salts of different valences a common determinant or closely related determinants are formed. It is suggested that this determinant is formed by chromium in the trivalent form.
Asunto(s)
Cromo , Dermatitis por Contacto/inmunología , Epítopos/análisis , Animales , Células Cultivadas , Fenómenos Químicos , Química , Femenino , Cobayas , Linfocitos/inmunología , Masculino , Relación Estructura-ActividadRESUMEN
Guinea pigs sensitized intradermally with haptenized macrophages exhibit a positive skin reaction to an epicutaneous challenge with the specific hapten only when syngenic macrophages were used. Skin reactions to the hapten were in guinea pigs treated with haptenized allogenic macrophages always negative. There are two possible explanations: (1) T-lymphocytes are unable to recognize the hapten on allogenic macrophages, and consequently the animal is not hypersensitive. (2) The antigenic complex used for sensitization (haptenized allogenic macrophages) is not identical with the complex formed during the epicutaneous challenge with the hapten in respect of the carrier. Therefore, the skin reaction to the hapten remains negative.