RESUMEN
The epidemic of bovine spongiform encephalopathy (BSE) in the United Kingdom, its related occurrence of a new type of Creutzfeld-Jacob disease and proven cases of this type of the disease transmitted by blood transfusion initiated intensive studies to develop a inexpensive, prompt, and sensitive method for the early lifetime diagnosis of prion diseases. This would permit initiation of the timely treatment of the patients and prevention of contamination of foodstuffs. However, despite significant progress made in this direction, this objective has not yet been achieved. The present review highlights the currently available methods for the diagnosis of transmissible spongiform encephalopathies, as well as the latest developments in the ultrasensitive detection of these diseases, which is based on the misfolded prion protein complex.
Asunto(s)
Enfermedades por Prión/diagnóstico , Priones/análisis , Animales , Biomarcadores/análisis , Biomarcadores/sangre , Biomarcadores/metabolismo , Encéfalo/metabolismo , Encéfalo/patología , Bovinos , Células Cultivadas , Síndrome de Creutzfeldt-Jakob/diagnóstico , Síndrome de Creutzfeldt-Jakob/metabolismo , Síndrome de Creutzfeldt-Jakob/patología , Humanos , Immunoblotting , Microscopía Fluorescente , Enfermedades por Prión/metabolismo , Enfermedades por Prión/patología , Priones/sangre , Priones/metabolismoAsunto(s)
Amiloide/metabolismo , Priones/metabolismo , Proteínas Recombinantes/metabolismo , Amiloide/química , Animales , Bovinos , Electroforesis en Gel de Poliacrilamida , Humanos , Concentración de Iones de Hidrógeno , Priones/química , Estructura Secundaria de Proteína , Proteínas Recombinantes/química , Factores de TiempoRESUMEN
Full-length Bos taurus PrPC protein was obtained in the eu- and prokaryotic expression systems. Immunoblotting and indirect enzyme immunoassay demonstrated high specificity and antigenic activity of full-length proteins in the reactions with monoclonal antibodies (anti-SAF-32 and VRQ-84). Membrane location of recombinant PrPC protein in insect cells was shown by immunofluorescent analysis.