RESUMEN
Superficial angiomyxoma is an infrequent benign soft tissue tumor, clinically presented as slow-growing, painless cutaneous, or subcutaneous mass. It is mostly described in middle aged population with preference location in trunk, head, neck, and genitalia. Herein, we report a rare growth variant of a solitary superficial angiomyxoma with uncommon site of occurrence in a 39-year-old female. Pedunculated superficial angiomyxoma originating in anal canal is extremely rare. Histological features of the lesion comprising of spindle-shaped and stellate cells dispersed in myxoid stroma and prominent thin-walled blood vessels defined the diagnosis of superficial angiomyxoma. Tumor cells with immunohistochemistry analysis showed positivity for vimentin and CD34 and negativity for S100, while Ki-67 showed a low proliferation index. The recurrence of superficial angiomyxoma is previously described, but in our case no signs of recurrence were seen in follow-up for a period of 6 months. Superficial angiomyxoma should be considered as differential diagnosis with other polypoid or nodular masses in anal canal.
RESUMEN
Cancer is a genomic disease associated with accumulation of genetic damage. Cancer-initiating events, such as chromosome breakage, loss and rearrangement, can be used as biomarkers to evaluate individual cancer risk. Cytokinesis-block micronucleus cytome (CBMN - Cyt) assay parameters in peripheral blood lymphocytes (PBL) of thirty four patients diagnosed with high-grade squamous intraepithelial lesions (HSIL) and fifteen healthy women were measured. The genomic instability of patients diagnosed with HSIL were investigated in order to compare differences between the two subgroups of HSIL (CIN 2 and CIN 3). The micronucleus (MN) frequencies in PBL, as well as the frequencies of nucleoplasmic bridges (NPB) and nuclear buds (NBUD) were higher in patients than in controls (Mann- Whitney test, p < 0.05). These results provide evidence that CBMN cytome assay in peripheral blood lymphocytes may be used to identify individuals who are at high risk of developing cervical cancer. Since the extent of DNA damage varies between CIN 2 and CIN 3, these findings support the CIN grading system.
Asunto(s)
Inestabilidad Genómica/genética , Linfocitos/patología , Lesiones Intraepiteliales Escamosas/genética , Lesiones Intraepiteliales Escamosas/patología , Adulto , Anciano , Núcleo Celular/genética , Daño del ADN/genética , Femenino , Humanos , Pruebas de Micronúcleos/métodos , Persona de Mediana Edad , Neoplasias del Cuello Uterino/genética , Neoplasias del Cuello Uterino/patologíaRESUMEN
Bladder urothelial cell carcinoma (UCC) is an increasingly prevalent cancer worldwide, and thus, gaining a better understanding of its identifiable risk factors is a global priority. This study addressed this public health need with the understanding that cancer-initiating events, such as chromosome breakage, loss and rearrangement, can be reasonably used as biomarkers to evaluate an individual's cancer risk. Overall, forty bladder cancer patients and twenty controls were evaluated for genomic instability. To the best of the investigators' knowledge, this is the first study to perform micronucleus (MN) assays simultaneously in urothelial exfoliated cells (UEC), buccal exfoliated cells (BEC), and peripheral blood lymphocytes (PBL) in first-diagnosed, non-smoker bladder UCC patients. Additionally, the frequency of nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in PBL was evaluated. The MN frequencies in UEC, BEC, and PBL, as well as the frequencies of NPBs and NBUDs, were significantly higher in patients than in controls. In conclusion, MN assays, particularly in UEC, may be used to identify individuals who are at high risk of developing UCC, as single or as additional triage test to UroVysion FISH test. Our results further validate the efficacy of biomarkers, such as MN, NPBs, and NBUDs, as predictors of genomic instability.
Asunto(s)
Biomarcadores de Tumor/análisis , Carcinoma/patología , Núcleo Celular/patología , Células Epiteliales/patología , Inestabilidad Genómica , Neoplasias de la Vejiga Urinaria/patología , Urotelio/patología , Adulto , Anciano , Humanos , Linfocitos/patología , Masculino , Persona de Mediana Edad , Mucosa Bucal/patologíaRESUMEN
BACKGROUND AND AIM: The loss of genomic stability plays an important role in carcinogenesis. Therefore, it is imperative to use certain biomarkers of DNA damage due to genomic instability in order to predict cancer risk. The aim of this study was the evaluation of genomic instability in patients with cervical lesions. MATERIALS AND METHODS: We investigated the genetic damages in 80 subjects: 40 patients with high-grade squamous intraepithelial lesions (HSIL), 20 patients with invasive squamous cervical cancer (SCC) and 20 healthy women with a biomarker in two different tissues; the micronucleus (MN) test in peripheral blood lymphocytes (PBL), and in buccal exfoliated cells (BEC). This study also examined the frequency of other nuclear anomalies such as nucleoplasmic bridges (NPBs) and nuclear bunds (NBUDs) in PBL. RESULTS: The frequency of MN in BEC, MN in PBL, NPB in PBL and NBUD in PBL were significantly higher (p < 0.001), in patients compared to controls. The DNA damages in BEC and PBL were correlated positively with histological grade of cervical lesions. CONCLUSION: Although larger studies are needed, our data support the predictive value of MN, NPB and NBUD as biomarkers of genomic instability for evaluation of risk level of cancer diseases.