RESUMEN

The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.

Asunto(s)

Butirilcolinesterasa , Compuestos de Oro/síntesis química , Nanopartículas del Metal , Butirilcolinesterasa/química , Butirilcolinesterasa/aislamiento & purificación , Estabilidad de Enzimas , Compuestos de Oro/química , Humanos , Concentración de Iones de Hidrógeno , Nanopartículas del Metal/química , Tamaño de la Partícula