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The spatial and temporal evolution of the field backscattered by a beating heart while illuminated with a coherent light reveals its macro- and microvascularization in real time. To perform these vascularization images, we use a recently published method of laser speckle imaging, based on the selective detection of spatially depolarized speckle field that is mainly generated by multiple scattering. We consider the calculation of the speckle contrast, by a spatial or temporal estimation. We show that the signal-to-noise ratio of the observed vascular structure can be noticeably increased by a postprocessing method implying the calculation of a motion field that allows the selection of similar frames extracted from different heartbeat periods. This later optimization reveals vascular microstructures with a spatial resolution of the order of 100 µ m .

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RESUMEN

SIGNIFICANCE: We propose a technique devoted to real-time high-resolution imaging of skin microvascularization. AIM: The process utilizes the temporal variation of the spatially depolarized optical speckle field generated by moving red blood cells when illuminated with fully polarized coherent light. APPROACH: Polarimetric filtering prevents the contribution of surface scattering from reaching the camera and thus favors the detection of multiscattered photons from the deeper layers of the skin. RESULTS: Full-field images reveal the microvasculature with a spatial resolution of 80 µm. The acquisition speed allows for real-time applications. CONCLUSIONS: We demonstrate the ability of this method to determine in 1 s a stable and reliable microvascular activity, enabling numerous clinical applications that require quantitative measurements.

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We present an ultracompact infrared cryogenic camera integrated inside a standard Sofradir's detector dewar cooler assembly (DDCA) whose field of view is equal to 120°. The multichannel optical architecture produces four nonredundant images on a single SCORPIO detector with a pixel pitch of 15 µm. This ultraminiaturized optical system brings a very low additional optical and mechanical mass to be cooled in the DDCA: the cool-down time is comparable to an equivalent DDCA without an imagery function. Limiting the number of channels is necessary to keep the highest number of resolved points in the final image. However, optical tolerances lead to irregular shifts between the channels. This paper discusses the limits of multichannel architectures. With an image-processing algorithm, the four images produced by the camera are combined to process a single full-resolution image with an equivalent sampling pitch equal to 7.5 µm. Experimental measurements on the modulation transfer function and noise equivalent temperature difference show that this camera achieves good optical performance.