RESUMEN
Ongoing cortical activity consists of sequences of synchronized bursts, named neuronal avalanches, whose size and duration are power law distributed. These features have been observed in a variety of systems and conditions, at all spatial scales, supporting scale invariance, universality and therefore criticality. However, the mechanisms leading to burst triggering, as well as the relationship between bursts and quiescence, are still unclear. The analysis of temporal correlations constitutes a major step towards a deeper understanding of burst dynamics. Here, we investigate the relation between avalanche sizes and quiet times, as well as between sizes of consecutive avalanches recorded in cortex slice cultures. We show that quiet times depend on the size of preceding avalanches and, at the same time, influence the size of the following one. Moreover we evidence that sizes of consecutive avalanches are correlated. In particular, we show that an avalanche tends to be larger or smaller than the following one for short or long time separation, respectively. Our analysis represents the first attempt to provide a quantitative estimate of correlations between activity and quiescence in the framework of neuronal avalanches and will help to enlighten the mechanisms underlying spontaneous activity.
Asunto(s)
Modelos Neurológicos , Neuronas/fisiología , Algoritmos , Animales , Corteza Cerebral/citología , Corteza Cerebral/fisiología , Modelos Teóricos , RatasRESUMEN
Neuronal avalanches, measured in vitro and in vivo, exhibit a robust critical behavior. Their temporal organization hides the presence of correlations. Here we present experimental measurements of the waiting time distribution between successive avalanches in the rat cortex in vitro. This exhibits a nonmonotonic behavior not usually found in other natural processes. Numerical simulations provide evidence that this behavior is a consequence of the alternation between states of high and low activity, named up and down states, leading to a balance between excitation and inhibition controlled by a single parameter. During these periods, both the single neuron state and the network excitability level, keeping memory of past activity, are tuned by homeostatic mechanisms.
Asunto(s)
Modelos Neurológicos , Neuronas/fisiología , Potenciales de Acción/fisiología , Animales , Red Nerviosa/fisiología , RatasRESUMEN
Fast-spiking (FS) interneurons provide the main route of feedforward inhibition from cortex to spiny projection neurons in the striatum. A steep current-firing frequency curve and a dense local axonal arbor suggest that even small excitatory inputs could translate into powerful feedforward inhibition, although such an arrangement is also sensitive to amplification of spurious synaptic inputs. We show that a transient potassium (KA) current allows the FS interneuron to strike a balance between sensitivity to correlated input and robustness to noise, thereby increasing its signal-to-noise ratio (SNR). First, a compartmental FS neuron model was created to match experimental data from striatal FS interneurons in cortex-striatum-substantia nigra organotypic cultures. Densities of sodium, delayed rectifier, and KA channels were optimized to replicate responses to somatic current injection. Spontaneous alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and gamma-aminobutyric acid (GABA) synaptic currents were adjusted to the experimentally measured amplitude, rise time, and interevent interval histograms. Second, two additional adjustments were required to emulate the remaining experimental observations. GABA channels were localized closer to the soma than AMPA channels to match the synaptic population reversal potential. Correlation among inputs was required to produce the observed firing rate during up-states. In this final model, KA channels were essential for suppressing down-state spikes while allowing reliable spike generation during up-states. This mechanism was particularly important under conditions of high dopamine. Our results suggest that KA channels allow FS interneurons to operate without a decrease in SNR during conditions of increased dopamine, as occurs in response to reward or anticipated reward.
Asunto(s)
Cuerpo Estriado/fisiología , Modelos Neurológicos , Red Nerviosa/fisiología , Canales de Potasio con Entrada de Voltaje/fisiología , Potasio/metabolismo , Transmisión Sináptica/fisiología , Animales , Simulación por Computador , Retroalimentación/fisiología , Humanos , Modelos EstadísticosRESUMEN
The subthalamic nucleus of the basal ganglia (STN) is important for normal movement as well as in movement disorders. Lesioning or deep-brain stimulation of the STN can alleviate resting tremor in Parkinson's disease. The STN and its target nuclei display synchronized oscillatory burst discharge at low frequencies, some of which correlate with tremor, but the mechanism underlying this synchronized bursting is unknown. Here we show that the excitatory STN and inhibitory, external globus pallidus (GPe) form a feedback system that engages in synchronized bursting. In mature organotypic cortex-striatum-STN-GPe cultures, neurons in the STN and GPe spontaneously produce synchronized oscillating bursts at 0.4, 0.8 and 1.8 Hz. Pallidal lesion abolishes this bursting, whereas cortical lesion favours bursting at 0.8 Hz. Pallidal bursts, although weaker than STN bursts, were required for synchronized oscillatory burst generation by recruitment of subthalmic rebound excitation. We propose that the STN and GPe constitute a central pacemaker modulated by striatal inhibition of GPe neurons. This pacemaker could be responsible for synchronized oscillatory activity in the normal and pathological basal ganglia.
Asunto(s)
Ganglios Basales/fisiología , Globo Pálido/fisiología , Tálamo/fisiología , Animales , Células Cultivadas , Sincronización Cortical , Dopamina/metabolismo , Antagonistas de Aminoácidos Excitadores , Ácido Glutámico/metabolismo , Inhibición Neural , Neuronas/fisiología , Quinoxalinas/farmacología , Ratas , Transmisión Sináptica/fisiología , Ácido gamma-Aminobutírico/metabolismoRESUMEN
The morphological organization of the globus pallidus (GP), the subthalamic nucleus (STN), and the pallidosubthalamic projection was studied in organotypic cultures. Coronal slices from the GP, the STN, the striatum (CPu), and the cortex (Cx) were taken from the rat after postnatal days 0-2 and grown for 2 or 5-6 weeks. For analysis, immunocytochemistry against glutamate (GLU), parvalbumin (PV), and calretinin (CR) was combined with confocal microscopy. After 2 weeks in vitro, the STN showed a densely packed, homogeneous GLU-immunoreactive (ir) cell population. Pallidal GLU-ir neurons were heterogeneous, consisting of large-sized weakly GLU-ir neurons and small-sized intensively GLU-ir neurons. After 5-6 weeks in vitro, pallidal axons had radiated from numerous large-sized PV-ir cells and selectively innervated the STN, where they heavily ramified. Cultured STN neurons were not stained for PV; however, multipolar intensely PV-ir neurons were located at the border of the STN with their dendrites oriented towards the STN. Double labeling for PV and CR in both mature cultures and in the adult rat revealed that the culture CR-ir neurons from the GP, the Cpu, and from areas adjacent to the STN were different from cultured PV-ir neurons and their morphologies and distribution corresponded to that in vivo. These results demonstrate that 1) cultured CP and STN neurons display similar morphologies found in in vivo, 2) PV-ir pallidal neurons heavily and selectively innervate the STN; 3) there is a specific class of STN border neurons; and 4) in contrast to the in vivo situation, most cultured STN neurons are PV-negative.
Asunto(s)
Globo Pálido/anatomía & histología , Globo Pálido/fisiología , Ratas/anatomía & histología , Ratas/fisiología , Núcleos Talámicos/anatomía & histología , Núcleos Talámicos/fisiología , Animales , Animales Recién Nacidos/anatomía & histología , Animales Recién Nacidos/fisiología , Calbindina 2 , Cuerpo Estriado/citología , Cuerpo Estriado/fisiología , Globo Pálido/citología , Ácido Glutámico/metabolismo , Inmunohistoquímica , Microscopía Confocal , Neuronas/fisiología , Técnicas de Cultivo de Órganos , Parvalbúminas/metabolismo , Ratas Sprague-Dawley , Proteína G de Unión al Calcio S100/metabolismo , Núcleos Talámicos/citologíaRESUMEN
Dopamine neurons in the substantia nigra heavily innervate the striatum, making it the nucleus with the highest levels of dopamine in the adult brain. The present study analyzes the time course and the density of striatal innervation by nigral dopamine neurons and characterizes the role of the neurotransmitter glutamate during the development of the nigrostriatal pathway. For this purpose, organotypic cultures containing the cortex, the striatum, and the substantia nigra (triple cultures) were prepared from rat brains at postnatal day (PND) 0-2 and were cultured for up to 60 d in vitro (DIV). Dopamine fibers and neurons were labeled using tyrosine hydroxylase (TH) immunohistochemistry. Striatal TH-ir fiber density was quantitatively analyzed using confocal laser scanning microscopy (CLSM). In long-term triple cultures (44 +/- 3 DIV), the striatal dopamine fiber density was high and was weakly correlated with the number of nigral dopamine neurons. The high striatal dopamine fiber density mainly resulted from an enhanced ingrowth and ramification of dopamine fibers from nigral neurons during 8-17 DIV. The metabotropic glutamate receptor (mGluR) antagonist L(+)-2-amino-3-phosphonopropionic acid (L-AP-3) selectively inhibited this dopaminergic innervation of the striatum, whereas ionotropic GluR antagonists had no effect. The L-AP-3-mediated inhibition was prevented by the mGluR agonist 1S, 3R-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD). The inhibition of the striatal dopaminergic innervation by L-AP-3 was further confirmed by anterograde tracing of the nigrostriatal projection with Phaseolus vulgaris leucoagglutinin. These results indicate that glutamate, by acting on group I mGluRs, plays an important "trophic" role for the development of the nigrostriatal dopamine pathway.
Asunto(s)
Cuerpo Estriado/citología , Neuronas/química , Receptores de Glutamato Metabotrópico/metabolismo , Sustancia Negra/citología , Alanina/análogos & derivados , Alanina/farmacología , Animales , Corteza Cerebral/química , Corteza Cerebral/citología , Cuerpo Estriado/química , Cuerpo Estriado/crecimiento & desarrollo , Cicloleucina/análogos & derivados , Cicloleucina/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Ácido Glutámico/metabolismo , Microscopía Confocal , Fibras Nerviosas/fisiología , Vías Nerviosas , Neuronas/enzimología , Neuronas/ultraestructura , Fármacos Neuroprotectores/farmacología , Técnicas de Cultivo de Órganos , Fitohemaglutininas , Ratas , Ratas Sprague-Dawley , Receptores de Glutamato Metabotrópico/agonistas , Receptores de Glutamato Metabotrópico/antagonistas & inhibidores , Sistemas de Mensajero Secundario/fisiología , Sustancia Negra/química , Sustancia Negra/crecimiento & desarrollo , Tirosina 3-Monooxigenasa/análisisRESUMEN
In vivo intracellular spontaneous activity in striatal medium spiny (MS) projection neurons is characterized by "up" and "down" states. How this type of activity relates to the neuronal activity of striatal fast-spiking (FS) interneurons was examined in the presence of nigral and cortical inputs using cortex-striatum-substantia nigra organotypic cultures grown for 45 +/- 4 d. The nigrostriatal projection was confirmed by tyrosine hydroxylase immunoreactivity. Corticostriatal (CS) projection neurons, striatal MS neurons, and FS neurons were intracellularly recorded and morphologically and electrophysiologically characterized. Intracellular spontaneous activity in the cultures consisted of intermittent depolarized periods of 0.5-1 sec duration. Spontaneous depolarizations in MS neurons were restricted to a narrow membrane potential range (up state) during which they occasionally fired single spikes. These up states were completely blocked by the glutamate antagonist CNQX. In FS interneurons, depolarized periods were characterized by large membrane potential fluctuations that occupied a wide range between rest and spike threshold. Also, FS interneurons spontaneously fired at much higher rates than did MS neurons. Simultaneous intracellular recordings established that during spontaneous depolarizations MS neurons and FS interneurons displayed correlated subthreshold neuronal activity in the low frequency range. These results indicate that (1) the CS projection neurons, striatal MS neurons, and FS interneurons grown in cortex-striatum-substantia nigra organotypic cultures show morphological and electrophysiological characteristics similar to those seen in vivo; (2) striatal MS neurons but not FS interneurons show an up state; (3) striatal MS neurons and FS interneurons receive common, presumably cortical inputs in the low frequency range. Our results support the view that the cortex provides a feedforward inhibition of MS neuron activity during the up state via FS interneurons.
Asunto(s)
Interneuronas/fisiología , Telencéfalo/citología , 6-Ciano 7-nitroquinoxalina 2,3-diona/farmacología , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Animales , Axones/fisiología , Técnicas de Cultivo de Célula/métodos , Células Cultivadas , Corteza Cerebral/citología , Cuerpo Estriado/citología , Dendritas/fisiología , Antagonistas de Aminoácidos Excitadores/farmacología , Interneuronas/efectos de los fármacos , Interneuronas/ultraestructura , Inhibición Neural/fisiología , Técnicas de Placa-Clamp , Ratas , Ratas Sprague-Dawley , Sustancia Negra/citología , Transmisión Sináptica/fisiologíaRESUMEN
1. Rhythmic cortical activity was investigated with intracellular recordings in cortex-striatum-mesencephalon organotypic cultures grown for 42 +/- 3 (SE) days in vitro. 2. Electrical stimulation of supragranular layers induced a self-sustained high-frequency oscillation (HFO) in pyramidal neurons and interneurons. 3. The HFO started 197 +/- 39 ms after stimulation and had a mean duration of 1.0 +/- 0.2 s and an initial frequency of 38 +/- 2 Hz. A decrease in frequency at a rate of 11.5 +/- 2.7 Hz/s started on average 547 +/- 109 ms after the onset of the HFO. 4. During the HFO, local interneurons and pyramidal neurons synchronized their activities. The synaptic origin of the HFO was confirmed by its reversal potential at -57 +/- 4 mV. 5. These results suggest that a self-maintained HFO can be induced in local cortical circuits by excitation of supragranular layers. This HFO would facilitate synchronization between distant cortical and thalamic regions.
Asunto(s)
Cuerpo Estriado/fisiología , Mesencéfalo/fisiología , Periodicidad , Corteza Somatosensorial/fisiología , Animales , Cuerpo Estriado/citología , Estimulación Eléctrica , Interneuronas/fisiología , Mesencéfalo/citología , Técnicas de Cultivo de Órganos , Células Piramidales/fisiología , Ratas , Ratas Sprague-Dawley , Corteza Somatosensorial/citologíaRESUMEN
We were successful in developing cortex-striatum-mesencephalon organotypic cultures from the rat brain after 4-9 weeks in vitro. A modification of the 'roller tube' technique was employed where slices were embedded in a plasma/thrombin clot onto a Millicell membrane on a coverslip. The underlying membrane provided high mechanical stability during culturing, which reduced the likelihood of deterioration of the cultures. Tyrosine hydroxylase immunoreactivity was used to label dopamine neurons and axonal innervation into the cortical and striatal culture. The electrophysiological responses of striatal medium-sized spiny neurons to cortical, striatal and mesencephalic stimulation were characterized.
Asunto(s)
Corteza Cerebral/fisiología , Mesencéfalo/fisiología , Neostriado/fisiología , Sustancia Negra/fisiología , Animales , Axones/fisiología , Biotina/análogos & derivados , Biotina/biosíntesis , Recuento de Células , Corteza Cerebral/citología , Electrofisiología , Inmunohistoquímica , Mesencéfalo/citología , Neostriado/citología , Vías Nerviosas/citología , Vías Nerviosas/fisiología , Técnicas de Cultivo de Órganos , Ratas , Ratas Sprague-Dawley , Sustancia Negra/citología , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
Neural dynamics in organotypic cortex-striatum co-cultures grown for three to six weeks under conditions of dopamine deficiency are described. Single neuron activities were recorded intra- and extracellularly, and spatiotemporal spreading of population activity was mapped using voltage-sensitive dyes. The temporal properties of spike firing were characterized by interspike interval histograms, autocorrelation and crosscorrelation. Cortical pyramidal neurons (n = 40) showed irregular firing with a weak tendency to burst or to oscillate. Crosscorrelations revealed strong near-coincident firing and synaptic interactions. Disinhibition was a notable feature in a strongly firing cortical interneuron. Cortical activity spread in the co-culture, thus inducing an overall, homogeneous depolarization in the striatal part. Striatal cells were divided into principal cells and type I and II secondary cells. Principal cells (n = 40) were similar to those reported previously in vivo. Spiking activity ranged from irregular spiking at very low rates to episodic bursting, with an average burst duration of 1 s. Interspike intervals were single-peaked. Intracellular recordings revealed characteristic, long-lasting subthreshold depolarizations ("enabled state") that were shortened by local muscarinic receptor blockade. During prolonged time periods in the "enabled state", locally applied bicuculline induced strong firing in most principal neurons. Striatal secondary type I neurons (n = 25) showed high spiking rates, single- and double-peaked interval histograms and low-threshold, short-lasting stereotyped bursting activity and occasional rhythmic bursting. The firing of these neurons was increased by bicuculline. Crosscorrelations showed synchronization of these cells with principal cell activity. Secondary type II neurons (n = 15) revealed tonic, irregular firing patterns similar to cortical neurons, except with occasional firing in doublet spikes. We conclude that under conditions of dopamine deficiency in corticostriatal co-cultures (i) the cortex induces the "enabled" state and typical bursting mode in striatal principal neurons; (ii) principal neurons are strongly inhibited during the "enabled" state; (iii) muscarinic activity, presumably from tonically active striatal cholinergic interneurons, stabilizes the "enabled" state; (iv) striatal GABAergic interneurons receives synaptic inhibition and take part in synchronized activity among striatal principal cells. Our results favor the view of the striatum as a lateral inhibition network.
Asunto(s)
Corteza Cerebral/fisiología , Cuerpo Estriado/fisiología , Interneuronas/fisiología , Células Piramidales/fisiología , Animales , Células Cultivadas , Dopamina/metabolismo , Potenciales de la Membrana/fisiología , Ratas , Ratas Sprague-Dawley , Ácido gamma-Aminobutírico/metabolismoRESUMEN
An in vitro system was established to analyse corticostriatal processing. Cortical and striatal slices taken at postnatal days 0-2 were co-cultured for three to six weeks. The anatomy of the organotypic co-cultures was determined using immunohistochemistry. In the cortex parvalbumin-positive and calbindin-positive cells, which resembled those seen in vivo, had laminar distributions. In the striatum, strongly stained parvalbumin-positive cells resembling striatal GABAergic interneurons and cholinergic interneurons were scattered throughout the tissue. The soma area of these interneuron classes was larger than the average striatal soma area, thus enabling visual selection of cells by class before recording. Cortical neurons with projections to the striatum showed similar morphological features to corticostriatal projection neurons in vivo. No projections from the striatum to the cortex were found. Intracellular recordings were obtained from 94 neurons. These were first classified on the basis of electrophysiological characteristics and the morphologies of cells in each class were reconstructed. Two types of striatal secondary neurons with unique electrophysiological dynamics were identified: GABAergic interneurons (n = 17) and large aspiny, probably cholinergic, interneurons (n = 15). The electrophysiological and morphological characteristics of cortical pyramidal cells (n = 27), cortical interneurons (n = 1), as well as striatal principal neurons (n = 34), were identical to those reported for similar ages in vivo. Organotypic cortex-striatum co-cultures are therefore suitable as an in vitro system in which to analyse corticostriatal processing. The network dynamics, which developed spontaneously in that system, are examined in the companion paper.
Asunto(s)
Corteza Cerebral/anatomía & histología , Corteza Cerebral/fisiología , Cuerpo Estriado/anatomía & histología , Cuerpo Estriado/fisiología , Interneuronas/fisiología , Animales , Células Cultivadas , Inmunohistoquímica , Técnicas In Vitro , Interneuronas/clasificación , Vías Nerviosas/fisiología , Células Piramidales/anatomía & histología , Células Piramidales/fisiología , Ratas , Ratas Sprague-DawleyRESUMEN
Spatio-temporal spreading of activity in the CA1 region of the rat hippocampal slice was studied by two experimental approaches. At identical locations in the tissue we measured both the extracellular field potential distribution with microelectrode recordings and the intracellular potential distribution by optical recording, using voltage-sensitive fluorescent dyes. Current source density analysis (CSD) was applied to the extracellular field potential distributions (eCSD) to enhance the spatial resolution. In order to obtain an analogous improvement for the optical recordings, we developed a new CSD transformation, which calculates the locations of the transmembrane current generators from the intracellular potential distributions (iCSD). Compared to the underlying fluorescence maps, the new iCSD profiles exhibit a considerable improvement in spatial resolution. Results can be directly interpreted in terms of physiological membrane processes, such as postsynaptic potentials and action potentials. The iCSD profiles show a surprisingly good correspondence with the classical eCSD profiles both qualitatively and quantitatively, the only difference being that cell body activity is reduced in amplitude. Thus, this new optical CSD analysis paves the way for a quantitative interpretation, rather than the hitherto predominantly qualitative interpretation of spatio-temporal activity profiles from optical recording measurements.