RESUMEN
Typha domingensis Pers. is known for its medicinal properties. Although traditionally T. domingensis Pers. has been used for wound healing, yet scientific investigations reporting its ability to heal wounds are lacking. Phytochemical profiling of T. domingensis Pers. inflorescence crude extract was carried out by LC-MS analysis. Ten phytochemicals were selected for in silico analysis based on retention time, mass-to-charge ratio and resolution of mass spectrum. Molecular docking of all ten compounds was done against selected wound healing biomarkers viz., interleukin 6(IL-6), interleukin ß (IL-ß), insulin-like growth factor tyrosine kinase receptor (IGF-1R) and transformation growth factor ß (TGF-ß). Based on this, catechin, mesalazine and piperazine were subjected for in vitro cell migration assay (3T3 L1 mouse fibroblast cell line) to assess their wound healing potentials. Molecular docking revealed that mesalazine, catechin, and piperazine have potential ligands based on lowest docking energy (ranging from - 4.1587 to - 0.972), Glide E score (ranging from - 26.929 to - 57.882), Glide G score (ranging from - 4.16 to - 7.972) and numbers of hydrogen bonds compared to other compounds studied. The migration assay revealed that, compared to control (52.5%), T. domingensis Pers. inflorescence crude extract showed maximum wound healing potential (80%) followed by Catechin (66.8%) Mesalazine (58.3%) and Piperazine (51.2%). The combined in silico and in vitro approach opens new dimension for designing innovative therapeutics to manage different types of wounds.
RESUMEN
It is both interesting and necessary to identify and develop nontoxic radioprotective compounds. Bleomycin (BLM), a known radiomimetic drug was used as a clastogen in the present study. The possible protective effects against BLM (15 µg/ml) induced clastogenicity by aqueous and methanolic extracts from Alstonia scholaris bark, stem and leaves were compared. The treatment of bark extracts significantly (p < 0.01) reduced total chromosomal aberrations. Such a reduction was not seen in case of stem and leaf treatments. The dose of 50 µg/ml was fixed for all extracts throughout the study. To understand the mechanism involved with the protective property of bark extracts, sensitive G2 assay was performed. Lymphocyte cultures from 12 healthy volunteers were exposed to aqueous (50 µg/ml) and methanolic (50 µg/ml) extracts of A. scholaris bark alone as well as in combination with Bleomycin under two different growth phases, G0 and G2. There was a statistically significant reduction (p < 0.05) in the total chromatid breaks in all cultures which were exposed at G2 phase as compared to respective cultures exposed at G0 phase. The highest level (p < 0.0001) of reduction in total chromatid breaks was observed in cultures treated with aqueous bark extracts at G2 phase than those at G0 phase. This indicated that there could be certain compound(s) present in aqueous bark extracts which enhance DNA repair capacity. Therefore, the bark of A. scholaris could be further utilized to identify and bring out front line radio protective agents in the market with effective formulations.