RESUMEN
1. SB-73, a magnesium ammonium phospholinoleate anhydride aggregate, exhibited antiviral action in vitro in the concentration range of 50 to 100 micrograms/ml against herpes simplex type 1, stomatitis vesicular virus, adenovirus type 5, and in vivo in the dose range of 0.7 to 1.3 mg/kg against canine parvovirus and distemper virus. 2. The lethal dose (LD50) was 2.71 +/- 1.55 g/kg body weight in mice inoculated intraperitoneally. Oral ingestion of the aggregate up to 30 g/kg body weight by mice had no lethal effects during the 14 days of observation. 3. In in vitro cytotoxicity experiments with fibroblasts (V-79 Chinese hamster cell line), no toxic effects were observed with SB-73 concentrations (120 micrograms/ml) having antiviral activity. 4. In a cellular proliferation experiment using hamster V-79 cells, we observed 72% proliferation after treatment of the cells with a high concentration (500 micrograms/ml) of SB-73. 5. Compound SB-73 showed no genotoxicity for human lymphocytes at concentrations of 100 micrograms/ml. 6. When the cytotoxicity and genotoxicity of SB-73 were compared with those of acyclovir, idoxuridine and AZT at 500 micrograms/ml concentrations the compound was found to have effects similar to those of acyclovir.
Asunto(s)
Antivirales/farmacología , Ácidos Linoleicos , Magnesio/farmacología , Compuestos Organofosforados , Polímeros/farmacología , Virus/efectos de los fármacos , Aciclovir/química , Aciclovir/farmacología , Animales , Antivirales/toxicidad , Aberraciones Cromosómicas , Femenino , Humanos , Idoxuridina/química , Idoxuridina/farmacología , Dosificación Letal Mediana , Magnesio/química , Masculino , Ratones , Índice Mitótico , Polímeros/química , Zidovudina/química , Zidovudina/farmacologíaRESUMEN
1. SB-73, a magnesium ammonium phospholinoleate anhydride aggregate, exhibited antiviral action in vitro in the concentration range of 50 to 100 µg/ml against herpes simplex type 1, stomatitis vesicular virus, adenovirus type 5, and in vivo in the dose range of 0.7 to 1.3 mg/Kg against canine parvovirus distemper virus. 2. The lethal dose (LD50) was 2.71 ñ 1.55 g/Kg body weight in mice inoculated intraperitoneally. Oral ingestion of the aggregate up to 30 g/Kg body weight by mice had no lethal effects during the 14 days of observation. 3. In in vitro cytotoxicity experiments with fibroblasts (V-79 Chinese hamster cell line), no toxic effects were observed with SB-73 concentrations (120 µg/ml) having antiviral activity. 4. In a cellular proliferation experimental using hamster V-79 cells, we observed 72% proliferation after treatment of the cells with a high concentration (500 µg/ml) of SB-73. 5. Compound SB-73 showed no genotoxicity for human lymphocytes at concentrations of 100 µg/ml. 6. When the cytoxicity and genotoxicity of SB-73 wee compared with those of acyclovir, idoxuridine and AZT at 500µg/ml concentration the compound was found to have effects similar to those of acyclovir