RESUMEN

OBJECTIVES: To determine the prevalence of myasthenia gravis (MG) and the rate of concurrent autoimmune diseases in patients with MG. DESIGN AND SETTING: Using the Swedish health and population registers, during the period 2005-2010, we conducted a nested case-control study of patients with MG (n = 2045) with five age- and sex-matched population-based controls per case. Register-based MG diagnosis was validated against the Stockholm MG Cohort. Similar nested case-control studies were conducted in patients with multiple sclerosis (MS), as a neuroinflammatory disease control, and siblings of patients with MG. MAIN OUTCOME MEASURE: Odds ratios (ORs) and their 95% confidence intervals (CIs) were calculated as a measure of the association between MG and other autoimmune diseases. RESULTS: The prevalence of MG was 24.8/100,000, and patients with MG had an increased risk of another autoimmune disease compared to controls (22.0% vs. 8.9%; OR: 2.82, 95% CI: 2.49-3.20); this risk was stronger amongst younger persons and women. Polymyositis/dermatomyositis, systemic lupus erythematosus and Addison's disease, three conditions regulated by the HLA-B8-DR3 haplotype, were most strongly associated with MG, especially early-onset disease. HLA typing in the Stockholm MG Cohort showed that early-onset MG was indeed dominated by HLA-B8-DR3. The risk of another autoimmune disease was increased in both patients with MS and siblings of patients with MG, compared to their respective controls, but to a lesser extent than in patients with MG. CONCLUSIONS: Our results suggest that MG shares risk factors with other autoimmune diseases, to a greater degree than MS, with a particular role of the HLA-B8-DR3 haplotype, especially amongst younger and female patients.

Asunto(s)

Enfermedades Autoinmunes/epidemiología , Adulto , Distribución por Edad , Anciano , Enfermedades Autoinmunes/complicaciones , Estudios de Casos y Controles , Femenino , Antígeno HLA-B8/genética , Haplotipos , Humanos , Masculino , Persona de Mediana Edad , Miastenia Gravis/complicaciones , Miastenia Gravis/epidemiología , Fenotipo , Prevalencia , Factores de Riesgo , Distribución por Sexo , Suecia/epidemiología

RESUMEN

OBJECTIVES: The molecular mechanisms underlying the regulation of the CD152 (CTLA-4) gene are largely unknown. Two single nucleotide polymorphisms (SNPs) located in the promoter region are suspected to contribute to the pathogenesis of myasthenia gravis (MG) through regulation of gene expression. SETTING, SUBJECTS AND DESIGN: One hundred and sixty-five unrelated Swedish-Caucasian patients with MG (103 females and 62 males, age 17 to 92 years) and 148 ethnically matched healthy individuals were studied. Gene typing of two SNPs (T/C(-1772) and A/G(-1661)) and quantification of soluble CD152 were performed in the patients. Besides the association studies, the function of these two SNPs is characterized. RESULTS: We present new genetic associations of two SNPs in the CD152 gene with human MG. These SNPs located in the promoter region are involved in transcriptional binding activity for Nuclear Factor I (NF-1) and c/EBPbeta, as demonstrated using chromatin immunoprecipitation and electromobility shift assay. MG patients with the T/C(-1772) polymorphism have elevated levels of sCD152 in sera. CONCLUSIONS: The two SNPs in the promoter region are associated with MG and might cause abnormal alternative splicing and affect the expression of CD152, thereby contributing to the pathogenesis of MG.

Asunto(s)

Antígenos CD/genética , Antígenos de Diferenciación/genética , Miastenia Gravis/genética , Polimorfismo de Nucleótido Simple/genética , Factores de Transcripción/metabolismo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Antígenos CD/metabolismo , Antígenos de Diferenciación/metabolismo , Antígeno CTLA-4 , Ensayo de Inmunoadsorción Enzimática , Femenino , Genética de Población , Humanos , Inmunosupresores , Masculino , Persona de Mediana Edad , Miastenia Gravis/etiología , Miastenia Gravis/metabolismo , Polimorfismo de Longitud del Fragmento de Restricción , Regiones Promotoras Genéticas , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa

RESUMEN

Multiple sclerosis (MS) is a chronic, progressive disease of the central nervous system (CNS) characterized by consistent myelin injury. Antibody-mediated death of oligodendrocytes is a pathological feature in a subset of MS patients and may be of relevance to disease pathogenesis. In myasthenia gravis (MG), acetylcholine receptors (AChR) situated at the neuromuscular endplate are destroyed by autoreactive antibodies. B-cell activating factor of the tumour necrosis factor (TNF) superfamily (BAFF) is essential for B-cell survival. Using flow cytometry, we evaluated the expression of three BAFF-binding receptors, namely, BAFF-receptor (BAFF-R), B-cell maturation antigen (BCMA), and transmembrane activator and calcium modulating and cyclophilin ligand interactor (TACI) in peripheral-blood lymphocytes. Nearly all CD19(+) B cells and CD19(+)CD27(+) memory B cells expressed BAFF-R. The intensity of BAFF-R expression was not statistically different in MS or MG compared with healthy controls. Very few T cells expressed BAFF-R. BCMA expression was strictly limited to B cells. Although both B and T cells expressed TACI, levels were much higher on B cells compared with levels on T cells. The percentages of B and T cells expressing BCMA and TACI did not differ significantly in MS or MG versus controls. We conclude that the expression of BAFF-binding receptors is not appreciably altered in MS or MG.

Asunto(s)

Receptor del Factor Activador de Células B/biosíntesis , Receptor del Factor Activador de Células B/genética , Esclerosis Múltiple/inmunología , Esclerosis Múltiple/metabolismo , Miastenia Gravis/inmunología , Miastenia Gravis/metabolismo , Adulto , Receptor del Factor Activador de Células B/fisiología , Femenino , Regulación de la Expresión Génica , Humanos , Masculino , Esclerosis Múltiple/genética , Miastenia Gravis/genética , Unión Proteica/inmunología

RESUMEN

OBJECTIVES: To measure clinical and immunological parameters in a patient with myasthenia gravis (MG) treated with antibodies against tumour necrosis factor-alpha (infliximab, Remicade). PATIENT AND METHODS: A patient with severe MG received repeated injections of infliximab. His muscle function score was monitored and the immunological parameters were followed using enzyme-linked immunosorbent assay, flow cytometry and radioimmunoassay. RESULTS: The patient improved in muscle fatigability tests and the levels of antibodies against the acetylcholine receptor decreased during treatment. The activation marker human leucocyte antigen-DR on CD4(+) T cells also decreased. CONCLUSION: Treatment with infliximab might be beneficial for patients with severe MG but demands careful monitoring of possible serious side-effects.

Asunto(s)

Anticuerpos Monoclonales/uso terapéutico , Factores Inmunológicos/uso terapéutico , Miastenia Gravis/inmunología , Miastenia Gravis/fisiopatología , Humanos , Infliximab , Masculino , Persona de Mediana Edad , Fuerza Muscular/fisiología , Miastenia Gravis/tratamiento farmacológico , Recuperación de la Función/fisiología , Resultado del Tratamiento

RESUMEN

OBJECTIVES: To describe two patients with myasthenia gravis (MG) and prolactinoma and analyze the associations between MG and prolactin (PRL) levels. DESIGN: Two case reports and a case-control study of PRL levels in 192 patients with MG. PARTICIPANTS: The Immunological Research Laboratory, Center for Molecular Medicine, Department of Medicine and the Department of Neurology, Karolinska Institutet, Stockholm, Sweden; St Petersburg Medical Academy for Postgraduate Studies, and St Petersburg State Medical Pediatric Academy, Russia. RESULTS: Two women with MG and thymic hyperplasia accompanied by prolactinomas are described. The levels of plasma PRL were raised in 101 women with MG, but not in 91 men. There was an association between high PRL levels and high levels of autoantibodies against the acetylcholine receptor. CONCLUSIONS: There is an association of MG with raised levels of PRL in women. PRL has stimulating effects on immune activation and the increased levels might thus be implied in the pathophysiology of MG.

Asunto(s)

Miastenia Gravis/complicaciones , Neoplasias Hipofisarias/complicaciones , Prolactina/sangre , Prolactinoma/complicaciones , Timoma/complicaciones , Adulto , Autoanticuerpos/inmunología , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Persona de Mediana Edad , Miastenia Gravis/inmunología , Miastenia Gravis/fisiopatología , Neuroinmunomodulación/inmunología , Sistemas Neurosecretores/inmunología , Sistemas Neurosecretores/fisiopatología , Neoplasias Hipofisarias/inmunología , Neoplasias Hipofisarias/fisiopatología , Prolactina/metabolismo , Prolactinoma/inmunología , Prolactinoma/fisiopatología , Receptores Colinérgicos/inmunología , Timectomía , Timoma/inmunología , Timoma/fisiopatología , Hiperplasia del Timo/complicaciones , Hiperplasia del Timo/inmunología , Hiperplasia del Timo/fisiopatología , Regulación hacia Arriba/inmunología

RESUMEN

Myasthenia gravis (MG) is commonly regarded as the prototype of an antibody-mediated, organ-specific autoimmune disease. Antibodies against the acetylcholine receptor (AChR) on the muscle endplate trigger its typical clinical manifestations of weakness and fatiguability. T-B cell interactions are thought to play a crucial role in the pathogenesis of MG. OX40 (CD134), a costimulatory molecule that is expressed on activated CD4+ T-cells, might contribute to the development or pathogenesis of immune-mediated diseases such as rheumatoid arthritis and graft-versus-host disease. In the present study, we investigated the expression of OX40 on CD4+ T-cells from patients with MG and healthy individuals. Results from 36 MG patients and 28 healthy controls revealed that more freshly isolated CD4+ T-cells from MG patients expressed OX40 than cells from healthy individuals. High levels of antibodies against the AChR, thymic hyperplasia and onset at an early age were associated with elevated expression of OX40. Upon activation by various concentrations of anti-CD3 antibodies, CD4+ T-cells from MG patients showed a tendency toward higher levels of OX40 expression than cells from healthy individuals. Given the role of OX40 in the immune system, we conclude that OX40 might contribute to the development of MG.

Asunto(s)

Linfocitos T CD4-Positivos/inmunología , Miastenia Gravis/inmunología , Receptores del Factor de Necrosis Tumoral/análisis , Adulto , Estudios de Casos y Controles , Femenino , Citometría de Flujo , Humanos , Masculino , Receptores OX40 , Estadísticas no Paramétricas

RESUMEN

Studies in experimental animal models of human autoimmune diseases have revealed that CD4(+)CD25(+) T regulatory (Tr) cells are of thymic origin and have potentials in preventing auto-aggressive immunity. Myasthenia gravis (MG) is the best-characterized autoimmune disease. Changes in the thymus are found in a majority of patients with MG. Thymectomy has beneficial effects on the disease severity and course in a substantial proportion of MG patients. But the occurrence and characteristics of Tr cells have not yet been defined in MG. We determined the frequencies and properties of circulating CD4(+)CD25(+) versus CD4(+)CD25(-) cells in MG patients and healthy controls (HCs), with special focus on the effect of thymectomy on CD4(+)CD25(+) cells. CD4(+)CD25(high) cells comprise only about 2% of blood lymphocytes in both MG patients and HCs. Frequencies of CD4(+)CD25(high) cells were similar in MG patients irrespective of treatment with thymectomy. CD4(+)CD25(+) cells in both MG patients and HCs are mainly memory T cells and are activated to a greater extent than CD4(+)CD25(-) cells, as reflected by high levels of CD45RO and human leucocyte antigen (HLA)-DR-positive cells. In both MG patients and HCs, CD4(+)CD25(+) cells also contained a high proportion of CD95-expressing cells as possible evidence of apoptosis-proneness. Upon stimulation with anti-CD3/CD28 monoclonal antibodies, CD4(+)CD25(+) cells responded more vigorously than CD4(+)CD25(-) cells in MG, irrespective of treatment with thymectomy, as well as in HCs. Although CD4(+)CD25(-) cells are mainly naïve T cells, in non-thymectomized MG patients, they are activated to a greater extent as reflected by higher expression of HLA-DR and CD95 on the surface compared to HCs. The data thus show that there is no deficiency of CD4(+)CD25(+) cells in MG, nor is the proportion of CD4(+)CD25(+) cells influenced by thymectomy.

Asunto(s)

Antígenos CD4/inmunología , Miastenia Gravis/inmunología , Receptores de Interleucina-2/inmunología , Linfocitos T/inmunología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Antígenos CD4/sangre , Femenino , Antígenos HLA-DR/sangre , Antígenos HLA-DR/inmunología , Humanos , Antígenos Comunes de Leucocito/sangre , Antígenos Comunes de Leucocito/inmunología , Masculino , Persona de Mediana Edad , Receptores de Interleucina-2/sangre , Timectomía , Receptor fas/sangre , Receptor fas/inmunología

RESUMEN

Two pairs of monozygotic twins, discordant for myasthenia gravis (MG) for more than 30 years, were studied regarding T cell and antibody reactivity against disease related autoantigens, the acetylcholine receptor, one idiotypic and one anti-idiotypic human monoclonal antibody. The healthy and myasthenic twins had very similar autoantibody repertoires. IgG fractions from both healthy and myasthenic twins had the same capacity to decrease the free acetylcholine receptor content in mice after passive transfer. In comparison with their myasthenic sisters, the healthy twins had lower T cell responses against the acetylcholine receptor.

Asunto(s)

Autoinmunidad/fisiología , Linfocitos B/inmunología , Miastenia Gravis/inmunología , Linfocitos T/inmunología , Adulto , Animales , Anticuerpos Antiidiotipos/sangre , Anticuerpos Antiidiotipos/inmunología , Anticuerpos Monoclonales/sangre , Anticuerpos Monoclonales/inmunología , Autoantígenos/metabolismo , Autoinmunidad/genética , Linfocitos B/virología , Células Sanguíneas/inmunología , Linfocitos T CD4-Positivos/metabolismo , Linfocitos T CD8-positivos/metabolismo , Línea Celular , Transformación Celular Viral/inmunología , Citocinas/metabolismo , Femenino , Estudios de Seguimiento , Antígenos HLA-DR/metabolismo , Herpesvirus Humano 4 , Humanos , Antígenos Comunes de Leucocito , Estudios Longitudinales , Ratones , Ratones Endogámicos C57BL , Persona de Mediana Edad , Miastenia Gravis/patología , Miastenia Gravis/virología , Receptores de Antígenos de Linfocitos T/genética , Receptores de Antígenos de Linfocitos T/inmunología , Receptores Colinérgicos/sangre , Receptores Colinérgicos/inmunología , Linfocitos T/virología , Estudios en Gemelos como Asunto , Gemelos Monocigóticos

Asunto(s)

Antígenos CD4/metabolismo , Linfocitos T CD4-Positivos/inmunología , Miastenia Gravis/inmunología , Receptores de Interleucina-2/metabolismo , Subgrupos de Linfocitos T/inmunología , Estudios de Casos y Controles , Recuento de Células , Centrifugación/métodos , Ficoll , Humanos , Leucocitos Mononucleares/metabolismo

RESUMEN

Forty-seven patients with the late borrelial manifestation acrodermatitis chronica atrophicans (ACA) and with objective neurological and/or neurophysiological findings were followed up after antibiotic treatment with dermatological, serological, neurological and neurophysiological controls. Despite a good therapeutic effect on ACA lesions, specific antibody values and symptoms of irritative nerve lesions, the objective neurological and neurophysiological findings of nerve deficit remained unchanged. There was no progress of neuropathy findings during the follow-up time. Our interpretation of the results is that the remaining neuropathy signs after treatment of ACA are neurological sequelae and not manifestations of persisting Borrelia infection.

Asunto(s)

Acrodermatitis/complicaciones , Acrodermatitis/tratamiento farmacológico , Antibacterianos/uso terapéutico , Borrelia burgdorferi/patogenicidad , Cefuroxima/uso terapéutico , Doxiciclina/uso terapéutico , Enfermedad de Lyme/complicaciones , Enfermedad de Lyme/tratamiento farmacológico , Penicilinas/uso terapéutico , Enfermedades del Sistema Nervioso Periférico/tratamiento farmacológico , Enfermedades del Sistema Nervioso Periférico/etiología , Acrodermatitis/fisiopatología , Adulto , Anciano , Anciano de 80 o más Años , Borrelia burgdorferi/efectos de los fármacos , Femenino , Estudios de Seguimiento , Humanos , Enfermedad de Lyme/fisiopatología , Masculino , Persona de Mediana Edad , Enfermedades del Sistema Nervioso Periférico/fisiopatología , Estudios Prospectivos , Factores de Tiempo

RESUMEN

A regional database of myasthenia gravis (MG) patients was used to estimate the prevalence and selected characteristics of the disease in the county of Stockholm, Sweden. The prevalence of MG was 14.1/100,000 (17.1 for women and 10.8 for men). The mean age at onset for women and men was 34.9 and 48.5 years, respectively. About 60% of patients were diagnosed within the first year after initial symptoms. Generalized MG was found in 79% of patients, and 10% had severe symptoms. Almost two thirds of the patients had undergone thymectomy, and 30% needed immunosuppressive treatment. The increase in the prevalence of MG since the 1960s probably reflects an improvement in prognosis and higher detection rates of patients with milder symptoms. A delay in diagnosis indicates that early signs and symptoms of MG are still not well known by all doctors.

Asunto(s)

Miastenia Gravis/epidemiología , Adolescente , Adulto , Edad de Inicio , Anciano , Anciano de 80 o más Años , Niño , Bases de Datos Factuales , Femenino , Humanos , Inmunosupresores/uso terapéutico , Masculino , Persona de Mediana Edad , Miastenia Gravis/patología , Pronóstico , Índice de Severidad de la Enfermedad , Factores Sexuales , Suecia/epidemiología

RESUMEN

The cytotoxic T lymphocyte associated protein 4 (CTLA-4) gene (Ctla-4) is a candidate gene for autoimmune disease. We here report results of two single nucleotide polymorphisms (SNPs) in the Ctla-4, a +49 A/G SNP in CDS1 and a C/T promoter SNP at position -318. There were no differences in these two SNPs between patients and healthy individuals. The frequency of allele G and genotype G/G at position +49 in CDS1 was increased in patients with thymoma when compared with patients with normal and hyperplastic thymic histopathology. Patients with the G/G genotype had signs of immune activation manifested as higher levels of serum IL-1beta and higher percentage of CD28(+) T lymphocytes. There was a strong linkage between the 86bp allele in the 3'-UTR and the A(+49) allele in CDS1. Our results suggest that the SNP at position +49 in CDS1 might be associated with the manifestations of MG.

Asunto(s)

Antígenos de Diferenciación/genética , Inmunoconjugados , Miastenia Gravis/genética , Polimorfismo de Nucleótido Simple , Regiones Promotoras Genéticas , Regiones no Traducidas 3' , Abatacept , Antígenos CD , Antígenos CD28/análisis , Antígeno CTLA-4 , Femenino , Frecuencia de los Genes , Genotipo , Humanos , Interleucina-1/sangre , Interleucina-12/sangre , Desequilibrio de Ligamiento , Masculino , Miastenia Gravis/inmunología , Suecia , Linfocitos T/inmunología , Timoma/genética , Hiperplasia del Timo/genética , Neoplasias del Timo/genética

RESUMEN

We measured the levels of sFas and sFasL in CSF and serum of HIV-1 infected patients and related them to AIDS dementia complex (ADC). Specimens were obtained from 51 HIV-1 infected individuals (29 with ADC) and 39 HIV negative individuals. The sFas was detectable in all sera and 98% of CSF specimens. Measurable levels of sFasL were found in 79% of the CSF and 98% of sera samples. According to the presence or absence of ADC, we observed significant differences in CSF sFas (median and IQR 116, 132 vs. 30, 23 pg/ml, P<0.001) and sFasL (median and IQR 127, 290 vs. 15, 73 pg/ml, P<0.001) levels. The sFas in serum differed significantly between HIV-1 infected subjects and non-infected controls (P<0.001), with no correlation to ADC. On the contrary, sFasL in serum differed among HIV-1 infected subjects according to clinical signs of ADC. In the cross-sectional study, the number of cells present in CSF and CD4+ T cell counts in blood did not correlate to the levels of CSF sFas and sFasL. Interestingly, the number of HIV RNA copies in CSF correlated significantly to the levels of CSF sFasL (P=0.001) but not to sFas in the same compartment. Antiretroviral therapy reduced viral load and sFas levels in CSF in the majority of patients. sFas is a useful marker for ADC diagnosis and follow-up during antiviral treatment.

Asunto(s)

Complejo SIDA Demencia/líquido cefalorraquídeo , Complejo SIDA Demencia/inmunología , VIH-1 , Glicoproteínas de Membrana/líquido cefalorraquídeo , Receptor fas/líquido cefalorraquídeo , Complejo SIDA Demencia/tratamiento farmacológico , Fármacos Anti-VIH/uso terapéutico , Barrera Hematoencefálica/inmunología , Recuento de Linfocito CD4 , Proteína Ligando Fas , Humanos , Solubilidad , Carga Viral

RESUMEN

To characterize the involvement of costimulatory pathways in the pathogenesis of myasthenia gravis (MG), a multiparameter flow cytometry assay was adopted to enumerate blood mononuclear cells (MNC) expressing CD28, CD80, CD86, CD40, and CD40L molecules in patients with MG and healthy subjects. Patients with MG had lower percentages of CD8(+)CD28(+) cells, augmented percentages of CD4(+)CD80(+), CD4(+)CD86(+), CD8(+)CD80(+), CD8(+)CD86(+), CD14(+)CD80(+), and CD14(+)CD86(+) cells, and similar levels of cells expressing CD40 and CD40L and of B cells expressing CD80 and CD86 compared to the controls. Patients with early onset of MG (<40 years) had lower percentages of CD3(+)CD86(+), CD4(+)CD86(+), CD8(+)CD86(+) T cells and CD20(+)CD86(+) B cells compared to those with late onset (>40 years). There was a positive correlation between the patients' age and percentages of CD86(+) cells. The data indicate that the CD28/CD80-CD86 costimulatory pathway is involved in MG. The high percentages of CD80 and CD86 positive T cells and monocytes may reflect persistent activation of T and B cells, whereas the low CD28 expression may be the result of chronic exposure to CD80 and CD86. These molecules could be the focus for new and improved immunomodulating therapies of MG.

Asunto(s)

Autoanticuerpos/sangre , Miastenia Gravis/inmunología , Miastenia Gravis/metabolismo , Receptores Colinérgicos/inmunología , Adulto , Anciano , Anciano de 80 o más Años , Anticuerpos Monoclonales , Antígenos CD/análisis , Antígenos CD/biosíntesis , Antígenos CD/inmunología , Linfocitos B/química , Linfocitos B/inmunología , Linfocitos B/metabolismo , Antígeno B7-1/análisis , Antígeno B7-1/biosíntesis , Antígeno B7-1/inmunología , Antígeno B7-2 , Biomarcadores , Antígenos CD28/análisis , Antígenos CD28/biosíntesis , Antígenos CD28/inmunología , Antígenos CD40/análisis , Antígenos CD40/biosíntesis , Antígenos CD40/inmunología , Ligando de CD40 , Femenino , Citometría de Flujo , Humanos , Masculino , Glicoproteínas de Membrana/análisis , Glicoproteínas de Membrana/biosíntesis , Glicoproteínas de Membrana/inmunología , Persona de Mediana Edad , Miastenia Gravis/diagnóstico , Linfocitos T/química , Linfocitos T/inmunología , Linfocitos T/metabolismo

RESUMEN

Myasthenia gravis (MG) is characterized by T cell-dependent autoantibodies to the acetylcholine receptor on the post-synaptic membrane of the neuromuscular junction. The production of autoantibodies is regulated by T cells via cytokines. To investigate the involvement of interferon gamma (IFN-gamma), tumour necrosis factor alpha (TNF-alpha), interleukin-4 (IL-4) and interleukin-10 (IL-10) in MG, the mRNA levels of these cytokines in peripheral blood mononuclear cells without stimulation in vitro were quantified by competitive reverse transcription polymerase chain reaction technique. The mRNA level of TNF-alpha was significantly lower (P = 0.0004) in the overall group of MG patients compared with controls. IL-10 was also lower in MG patients (P = 0.008), most markedly in non-thymectomized patients (P = 0.016). There were no significant differences in IFN-gamma and IL-4 between patients and healthy controls, but the mRNA levels of IL-4 in non-thymectomized patients was significantly lower than in controls (P = 0.02) and in thymectomized patients (P = 0.03). These results, reflecting the in vivo expression pattern of these cytokines in the periphery, suggest an altered cytokine expression at the systemic level in MG.

Asunto(s)

Interleucina-10/metabolismo , Leucocitos Mononucleares/metabolismo , Miastenia Gravis/sangre , ARN Mensajero/sangre , Factor de Necrosis Tumoral alfa/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Miastenia Gravis/metabolismo

RESUMEN

CD28 is required to promote T cell proliferation and cytokine production, while the cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) functions as a negative modulator for T cell activation. We previously reported that alleles with longer PCR products (designated as allele xx) in an (AT)n polymorphism in Ctla-4 are associated with myasthenia gravis with thymoma, while the shortest allele, 86, is negatively associated with the disease. Here, we demonstrate that serum IL-2 sRalpha increases parallel to the length of (AT)n in Ctla-4. Periphereal blood mononuclear cells (PBMC) from patients with Ctla-4 xx/xx contained higher activity of telomerase than patients bearing Ctla-4 86/86. Blockade of CTLA-4 increased the telomerase activity in PBMC stimulated by acetylcholine receptor in vitro. There was a positive correlation between the expression of CD28 and CTLA-4 on anti-CD3 activated PBMC, suggesting a balance between CD28 and CTLA-4. Cells from patients with Ctla-4 xx/xx had the highest level of T cell proliferative responses upon the addition of anti-CD28 antibodies to the anti-CD3 containing culture system while cells from patients with Ctla-4 86/xx had an intermediate and cells from patients with Ctla-4 86/86 the lowest increase. The current results point to the (AT)n in Ctla-4 as a myasthenia gravis facilitating mutation under certain permissive environments by influencing the T cell reactivity via the CD28 pathway.

Asunto(s)

Antígenos de Diferenciación/genética , Antígenos CD28/fisiología , Repeticiones de Dinucleótido , Inmunoconjugados , Activación de Linfocitos , Miastenia Gravis/inmunología , Linfocitos T/inmunología , Abatacept , Antígenos CD , Complejo CD3/inmunología , Antígeno CTLA-4 , Células Cultivadas , Humanos , Receptores de Interleucina-2/análisis , Telomerasa/metabolismo

RESUMEN

In a prospective study, detailed clinical and neurophysiological examinations were performed in 17 patients with polyneuropathy associated with the late borrelial manifestation acrodermatitis chronica atrophicans (ACA). Similar clinical and neurophysiological signs were found in most of the patients. The findings were those of a sensory polyneuropathy, mainly affecting large nerve fibres. Marked abnormality of vibration threshold was a common finding and in 4 patients this raised a suspicion of spinal cord engagement, in addition to a polyneuropathy. Sural nerve biopsy, performed in 3 of the patients, showed a mainly axonal neuropathy. Biopsy findings did not confirm earlier reports of vasculitis of epineural vessels in ACA-associated polyneuropathy.

Asunto(s)

Enfermedad de Lyme/diagnóstico , Polineuropatías/diagnóstico , Acrodermatitis/diagnóstico , Acrodermatitis/patología , Acrodermatitis/fisiopatología , Anciano , Anciano de 80 o más Años , Axones/patología , Axones/fisiología , Biopsia , Femenino , Humanos , Enfermedad de Lyme/patología , Enfermedad de Lyme/fisiopatología , Masculino , Fibras Nerviosas/patología , Fibras Nerviosas/fisiología , Examen Neurológico , Polineuropatías/patología , Polineuropatías/fisiopatología , Nervio Sural/patología , Nervio Sural/fisiopatología , Vasa Nervorum/patología , Vasa Nervorum/fisiopatología , Vasculitis/diagnóstico , Vasculitis/patología , Vasculitis/fisiopatología

RESUMEN

The beta2-adrenergic receptor (beta2-AR) belongs to the group of G-protein coupled receptors and is present mainly on skeletal and cardiac muscle cells and lymphocytes. The gene encoding beta2-AR (ADRB2) displays a moderate degree of heterogeneity in the human population. The distribution of polymorphisms at amino acid positions 16, 27 and 164 is changed in asthma, hypertension and obesity. We have earlier reported a decreased density of the beta2-AR on peripheral blood mononuclear cells and the presence of beta2-AR antibodies in patients with MG. Since certain polymorphisms affect the function of the beta2-AR, it was of interest to analyse these in MG. Using allele-specific polymerase chain reaction amplification, we revealed an over-representation of homozygosity for Arg16 and a lower prevalence of homozygosity for Gly16 in MG patients compared with healthy individuals. The increased frequency of homozygosity for Arg16 was due to a contribution from patients with generalized MG but not from patients with only ocular disease. Homozygosity for Glu27 was negatively associated with both the presence of beta2-AR antibodies and severity of disease. Moreover, acetylcholine receptor (AChR) antibodies were more often present in patients being homozygous for Gln27. Our results imply that homozygosity for Arg16 confers susceptibility to generalized MG, and that certain polymorphisms at amino acid position 27 are associated with subgroups of patients.

Asunto(s)

Miastenia Gravis/genética , Polimorfismo Genético , Receptores Adrenérgicos beta 2/genética , Adolescente , Adulto , Autoanticuerpos/sangre , Enfermedades Cardiovasculares/genética , Niño , Preescolar , Oftalmopatías/genética , Femenino , Homocigoto , Humanos , Inmunosupresores/uso terapéutico , Masculino , Persona de Mediana Edad , Miastenia Gravis/tratamiento farmacológico , Miastenia Gravis/inmunología , Receptores Adrenérgicos beta 2/inmunología

RESUMEN

We examined the bi-allelic polymorphism at - 174 in the promoter region and the polymorphism in the 3' flanking AT rich region of the interleukin-6 (IL-6) gene in Swedish patients with myasthenia gravis (MG) and ethnically matched healthy individuals. There was no association between the polymorphisms and the disease. There was no relation of the polymorphisms to the clinical variables, the thymic histopathologies, the level of serum acetylcholine receptor antibodies or the concentrations of IgG and its subclasses. Our data yield no evidence for the IL-6 gene contributing to the disease susceptibility.

Asunto(s)

Interleucina-6/genética , Miastenia Gravis/genética , Polimorfismo Genético , Secuencia Rica en At , Femenino , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina G/clasificación , Masculino , Miastenia Gravis/inmunología

RESUMEN

Antibodies to the nicotinic AChR are pivotal in the immunopathogenesis of MG. Cytokines produced by T-helper cells are important regulators of humoral immune responses. IL-10 is considered an anti-inflammatory cytokine, but it promotes B cell activation and worsens experimental autoimmune MG in Lewis rats, an experimental model of MG. To study IL-10 and, as a control, interferon-gamma (IFN-gamma) in MG, we used an enzyme-linked immunospot (ELISPOT) assay, thereby assessing numbers of blood mononuclear cells (MNC) secreting IL-10 and IFN-gamma spontaneously and after stimulation with AChR. Low numbers of IL-10-secreting cells were regularly found in peripheral blood from patients with MG as well as in controls with other neurological diseases and healthy subjects. However, only MG patients had elevated blood levels of AChR-reactive IL-10- and IFN-gamma-secreting cells. The MG patients showed no responses to the control autoantigen myelin basic protein, underlining the specificity of the IL-10 and IFN-gamma responses. Immunosuppressive treatment reduced numbers of AChR-reactive IFN-gamma-secreting cells but increased the numbers of IL-10-secreting cells. The numbers of IL-10-secreting cells tended to be higher in patients with generalized versus ocular MG, further suggesting that the augmented IL-10 responses may be important in the pathogenesis and perpetuation of MG.

Asunto(s)

Interleucina-10/sangre , Interleucina-10/metabolismo , Miastenia Gravis/inmunología , Receptores Colinérgicos/sangre , Receptores Colinérgicos/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Interferón gamma/inmunología , Leucocitos Mononucleares/inmunología , Leucocitos Mononucleares/metabolismo , Masculino , Persona de Mediana Edad