RESUMEN

OBJECTIVES: The aim of the study was to gain more insight into the relationship between transmitted singletons found at HIV diagnosis by population sequencing and the possible presence of clinically relevant viral minorities containing additional resistance mutations. METHODS: We studied the viral quasispecies and therapy response in 10 individuals with transmitted single nucleoside reverse transcriptase inhibitor (NRTI)-related resistance mutations as detected by population sequencing. RESULTS: Ultra-deep pyrosequencing did not reveal additional drug-resistance mutations in nine of 10 patients. In these nine patients, no breakthrough with resistant viruses was observed despite the use of low genetic nonnucleoside reverse transcriptase inhibitor (NNRTI)-based regimens in the majority of patients. CONCLUSIONS: These data suggest that viral minority variants containing additional resistance mutations may be rare in patients with transmitted NRTI singletons in the Netherlands. Larger studies are required to confirm these findings and to determine the therapeutic consequences.

Asunto(s)

Farmacorresistencia Viral/genética , Infecciones por VIH/genética , VIH-1/genética , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Inhibidores de la Transcriptasa Inversa/farmacología , Análisis de Secuencia de ARN/métodos , Productos del Gen pol del Virus de la Inmunodeficiencia Humana/genética , Adulto , Fármacos Anti-VIH/farmacología , Genotipo , Infecciones por VIH/tratamiento farmacológico , Infecciones por VIH/virología , Transcriptasa Inversa del VIH/genética , VIH-1/efectos de los fármacos , Homosexualidad Masculina , Humanos , Masculino , Persona de Mediana Edad , Mutación/genética , Países Bajos/epidemiología , ARN Viral/genética , Productos del Gen pol del Virus de la Inmunodeficiencia Humana/efectos de los fármacos