RESUMEN

The B. safensis RGM 2450 and B. siamensis RGM 2529 strains were isolated from the rhizosphere of plants presenting resilience to abiotic and biotic stress conditions. To understand the implications of bacteria in resilience, a genomic and experimental analysis was carried out on their biostimulant and phytopathogenic antagonist properties. Genome analyses of both strains indicated that they have the potential to synthesize bioactive compounds such as the battery of non-ribosomal peptides, polyketides, extracellular enzymes and phytohormones. These results were consistent with the antagonistic activities of both strains against the phytopathogens Botrytis cinerea, Colletotrichum acutatum, Fusarium oxysporum and Phytophtora cinnamomi. They also showed the capacity to solubilize phosphorus, fix nitrogen and produce indole acetic acid. This was observed in tomato seedlings grown from seeds inoculated with the mixture of strains which presented significantly greater length as well as wet and dry weight in comparison with the treatments individually inoculated with each strain and the control. Accordingly, the combination of B. safensis RGM 2450 and B. siamensis RGM 2529 showed synergistic biostimulant activity. These findings contribute new knowledge of the genomic and metabolomic properties taking part in the symbiotic interactions between these strains and the plants and uphold the combined use of both strains as a biostimulant.

RESUMEN

Introduction: Bone defects hinder implant positioning. Vertical bone deficiency is the most challenging for clinical treatment, due to the high sensitivity of the technique and frequent intra- and postoperative complications. An alternative treatment for vertical defects is guided bone regeneration with simultaneous implantation; however, few studies have evaluated its effectiveness over time. Objective: To evaluate peri-implant tissue stability in a dental implant simultaneously positioned in an aesthetic zone of an area of zone vertical bone regeneration 3 years after functional load. Case Presentation: A 62-year-old male presented with the absence of the right lateral incisor and vertical bone defect of 3mm. His initial condition affected the relation of zenith points, causing disharmony. After case evaluation, vertical guided bone regeneration and simultaneous dental implants were performed to obtain a vertical bone gain of 3mm. The implant was rehabilitated with a zirconium crown and evaluated 3 years after functional loading. Conclusion: Vertical guided bone regeneration and simultaneous dental implant in the aesthetic zone in small bone defects (<4 mm) is an appropriate surgical and prosthetic technique to reduce surgical time, providing stability of peri-implant tissues even 3 years after functional implant loading.

Introducción: La presencia de defectos óseos dificulta el posicionamiento de los implantes, y es la deficiencia ósea vertical la que representa un mayor reto para el clínico por la alta sensibilidad de la técnica y las frecuentes complicaciones intra y posoperatorias. Una alternativa de tratamiento para los defectos verticales es la regeneración ósea guiada con implante simultáneo; sin embargo, en la literatura existen pocos estudios que evalúen su efectividad en el tiempo. Objetivo: Evaluar la estabilidad de los tejidos periimplantares en un implante dental posicionado simultáneo a la regeneración ósea vertical en una zona estética, luego de 3 años de carga funcional. Presentación de caso: Paciente masculino de 62 años que presenta ausencia del incisivo lateral derecho con un defecto óseo vertical de 3 mm, que crea una desarmonía de los puntos cenit. Posteriormente a la adecuada evaluación del caso, se decide realizar una regeneración ósea guiada vertical y colocar un implante dental simultáneo, y se obtuvo una ganancia ósea vertical de 3 mm. El implante es rehabilitado con corona en zirconio y evaluado luego de 3 años de la carga funcional. Conclusión: La regeneración ósea guiada vertical y el implante dental simultáneo en zona estética en defectos óseos pequeños (<4 mm) es una técnica quirúrgica y protésica adecuada para reducir los momentos quirúrgicos, ya que proporciona estabilidad a los tejidos periimplantares, luego de 3 años de la carga funcional del implante.