RESUMEN

Argonaute are a conserved class of proteins central to the microRNA pathway. We have highlighted a novel and non-redundant function of Ago1 versus Ago2; the two core factors of the miRNA-associated RISC complex. Stable overexpression of Ago1 in neuroblastoma cells causes the cell cycle to slow down, a decrease in cellular motility and a stronger apoptotic response upon UV irradiation. These effects, together with a significant increase in p53 levels, suggest that Ago1 may act as a tumor-suppressor factor, a function also supported by GEO Profiles microarrays that inversely correlate Ago1 expression levels with cell proliferation rates.

Asunto(s)

Apoptosis/fisiología , Proteínas Argonautas/metabolismo , Movimiento Celular/fisiología , Proliferación Celular , Factores Eucarióticos de Iniciación/metabolismo , Proteínas Argonautas/genética , Ciclo Celular/fisiología , Diferenciación Celular/fisiología , Línea Celular Tumoral , Factores Eucarióticos de Iniciación/genética , Humanos , Análisis por Micromatrices , Neuroblastoma , Neuronas/fisiología , Proteína p53 Supresora de Tumor/genética , Proteína p53 Supresora de Tumor/metabolismo