RESUMEN
A simple, sensitive and label-free optical sensor method was developed for allergens analysis using α-casein as the biomarker for cow's milk detection, to be used directly in final rinse samples of cleaning in place systems (CIP) of food manufacturers. A Surface Plasmon Resonance (SPR) sensor chip consisting of four sensing arrays enabling the measurement of samples and control binding events simultaneously on the sensor surface was employed in this work. SPR offers several advantages in terms of label free detection, real time measurements and superior sensitivity when compared to ELISA based techniques. The gold sensor chip was used to immobilise α-casein-polyclonal antibody using EDC/NHS coupling procedure. The performance of the assay and the sensor was first optimised and characterised in pure buffer conditions giving a detection limit of 58ngmL-1 as a direct binding assay. The assay sensitivity can be further improved by using sandwich assay format and amplified with nanoparticles. However, at this stage this is not required as the detection limit achieved exceeded the required allergens detection levels of 2µgmL-1 for α-S1-casein. The sensor demonstrated good selectivity towards the α-casein as the target analyte and adequate recoveries from CIP final rinse wash samples. The sensor would be useful tool for monitoring allergen levels after cleaning procedures, providing additional data that may better inform upon wider food allergen risk management decision(s) that are made by food manufacturer. In particular, this sensor could potentially help validate or optimise cleaning practices for a given food manufacturing process.
Asunto(s)
Alérgenos/análisis , Anticuerpos Inmovilizados/química , Caseínas/análisis , Resonancia por Plasmón de Superficie/métodos , Animales , Bovinos , Oro/química , Inmunoensayo/métodos , Límite de Detección , Leche/químicaRESUMEN
Perivascular tissue (PVT) modulates vascular tone, releasing adventitia/adipocyte derived relaxing factor (ADRF). Its physiological role remains unclear. We studied isolated internal thoracic artery (ITA) segments obtained from 132 patients subjected to coronary artery bypass grafting. The vessels were skeletonized in vitro and the ITA rings and PVT were incubated in separate isolated organ baths. Skeletonized ITA segments were first precontracted with 10(-5.5)mol/L 5-hydroxytryptamine hydrochloride. The PVT was next transferred to the ITA tissue bath. This resulted in relaxation of ITA, presumably related to ADRF release from PVT which was floating freely in the tissue bath. The in-vitro relaxation responses were then correlated to patients' characteristics - including demographics, clinical and laboratory data, as well as therapy. Perivascular tissue transfer resulted in 49.7 ± 26.2% relaxation of precontracted ITA segments. In multiple linear regression modelling, the relaxation of ITAto PVT was negatively related to patient age (ß = -0.67; 95% CI -1.17 - -0.17; P = 0.009), symptoms of CCS class 4 angina (ß = -20.11; 95%CI -32.25 - -7.97; P = 0.001), and positively to body mass (ß = 0.37; 95%CI 0.08 - 0.67; P = 0.01) and lack of heart failure symptoms (NYHA class 1) (ß = 9.06; 95%CI 0.33 - 17.79; P = 0.04). The relaxation response to PVT was not related to patients' sex, diabetes, hypertension, lipid profile or therapy in both univariate and multivariate analysis. PVT might play an important role in regulating vascular tone in humans as exemplified by its changing physiological function with age and in atherosclerosis.
Asunto(s)
Tejido Conectivo/fisiología , Arterias Mamarias/fisiología , Estructuras Creadas Quirúrgicamente/fisiología , Vasodilatación/fisiología , Anciano , Puente de Arteria Coronaria , Femenino , Humanos , Técnicas In Vitro , Masculino , Arterias Mamarias/efectos de los fármacos , Arterias Mamarias/cirugía , Persona de Mediana Edad , Serotonina/farmacologíaRESUMEN
Radiculographic assessment and intraoperative findings in patients with single level lumbar disc nucleus pulposus herniation have been compared with each another. Radiculography has been confirmed in 81 per cent of cases.
Asunto(s)
Desplazamiento del Disco Intervertebral/diagnóstico por imagen , Vértebras Lumbares/diagnóstico por imagen , Raíces Nerviosas Espinales/diagnóstico por imagen , Adolescente , Adulto , Anciano , Femenino , Humanos , Periodo Intraoperatorio , Masculino , Persona de Mediana Edad , Mielografía/métodosRESUMEN
We measured the temperature of arterial blood within the circle of Willis (T(Willis)) in seven normothermic patients undergoing carotid arteriography. We found this temperature to be lower by 0.13 to 0.30 degrees C than tympanic temperature and by 0.30 to 0.55 degrees C lower than rectal temperature. By imposing this data on calculated cerebral heat balance it can be suggested that the average cerebral temperature of a resting subject is somewhat higher than tympanic temperature. These findings would also support indirectly the thesis that temperature gradients may exist within the brain.