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This study aimed to evaluate and compare the shaping abilities and minimum dentin thickness of minimally invasive rotary instruments via micro-computed tomography. Twelve 3D-printed C-shaped canal models from a mandibular molar were divided into two groups, and root canals were prepared with either XP-endo Rise (XR) or TruNatomy (TN) systems. Pre- and post-preparation evaluations included canal volume, prepared area and minimum dentin thickness. No significant differences were found in canal volume change (XR: 22.66 ± 4.28%, TN: 23.02 ± 5.10%), prepared canal area (XR: 31.86 ± 10.72%, TN: 30.26 ± 11.59%) and minimum dentin thickness (XR: 0.30 ± 0.05 mm, TN: 0.28 ± 0.05 mm) between groups (p > 0.05). The canal volume change in the middle third was significantly higher than that in the coronal and apical thirds (p < 0.05) in both groups. In conclusion, XR demonstrated comparable shaping abilities and minimum dentin thickness to TN in preparing 3D-printed C-shaped canals.
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Introduction: Nanoparticulate Ca(OH)2 had greater antibacterial effect than conventional Ca(OH)2. Conversely, a study reported that nanoparticulate Ca(OH)2 had toxicity against murine fibroblast. However, the study of nanoparticulate Ca(OH)2, involving human dental pulp cells (DPCs) and apical papilla cells (APCs) is lacking. The aim of this study is to compare the effects of conventional Ca(OH)2 and nanoparticulate Ca(OH)2 on the viability of DPCs and APCs. Methods: Primary human DPCs/APCs from the 3rd to 5th passage were divided into control and experimental groups. In the control group, cells were cultured in complete media. In the experimental group, cells were cultured in complete media containing 10, 100, or 1000 µg/mL of either conventional Ca(OH)2 or nanoparticulate Ca(OH)2 for 1, 3, 5, and 7 days. After the treatment period, the cells were tested for viability using MTT assay. Results: DPCs treated with conventional Ca(OH)2 in all concentrations at day 5 revealed significantly higher proliferation compared to nanoparticulate Ca(OH)2 treated groups. In additions, DPCs treated with 1000 µg/ml nanoparticulate Ca(OH)2 at day7 were significantly lower proliferation compared to DPCs treated with conventional Ca(OH)2. In contrast, APCs treated with 1000 µg/ml nanoparticulated Ca(OH)2 were significantly higher proliferation than APCs treated with 1000 µg/ml conventional Ca(OH)2 at day7. Conclusions: Nanoparticulate Ca(OH)2 increased the viability of APCs and can be an alternative choice of intracanal medication for regenerative endodontic procedures. However, Nanoparticulate Ca(OH)2 exerted some effects on DPCs. The use of nanoparticulate Ca(OH)2 has no advantages over the conventional Ca(OH)2 for vital pulp therapy.
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OBJECTIVE: This study examined the chemical surface modification methods of resin composite repaired with resin-modified glass-ionomer cement (RMGIC). MATERIALS AND METHODS: Ninety aged resin composite rods were produced and sorted into 9 groups of 10 specimens and surface modified with silane agent and/or bonding agent as follows: group 1, no surface modified; group 2, etch + single bond 2 (SB2); group 3, SB2; group 4, etch + RelyX ceramic primer (RXP) + SB2; group 5, RXP + SB2; group 6, etch + single bond universal (SU); group 7, SU; group 8, etch + RXP + SU; and group 9, RXP + SU. A clear silicone mold was placed on the top of specimen center, and then filled with RMGIC. The specimens' shear bond strengths (SBSs) were examined in mechanical testing equipment. To determine failure types, the fractured specimen surfaces were inspected using a stereomicroscope. STATISTICAL ANALYSIS: The data collected were analyzed using one-way analysis of variance, and significance level was operated using Tukey's test (p < 0.05). RESULTS: Group 8 had the greatest SBS, but it was statistically indistinguishable from groups 4, 5, and 9. The most frequent fracture mode was adhesive failure. High SBS was commonly associated with mixed failure. CONCLUSION: The use of bonding agents enhances the resin composite's wettability and allows it to bond to RMGIC. Moreover, the use of the silane coupling agent before applying bonding agent showed significantly higher bonding ability of resin composite and RMGIC interface.
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This study determined the effect of lip thickness, lipstick color, and tooth shade on the smile attractiveness perceptions of dentists, laypersons, dental students, and other faculty students. A set of 27 smile photographs was prepared with different lip thicknesses (Tk, thick; M, medium; and Tn, thin), lipstick shade (R, red; P, pink; and O, orange), and tooth shades (0M1, 0M3, and A1). A total of 212 Thai participants in four rater groups (dentists, laypersons, dental students, and other faculty students) rated smile attractiveness using a visual analog scale (VAS). Statistical analyses were performed using the Kruskal-Wallis test and pairwise analysis at a 0.05% level of significance. Tk or M lip thickness was associated with more smile attractiveness than Th lip thickness. The R lipstick is more attractive than the P and O lipsticks. The 0M1 tooth shade appeared to be the most attractive for laypersons and other faculty students, whereas tooth shades (0M1, 0M3, or A1) did not influence the smile attractiveness perception of dentists and dental students. The smile attractiveness perception was influenced by the lip appearance and tooth shade for each rater group, which are essential for an attractive smile design.
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INTRODUCTION: A 3-antibiotic combination (3Mix) has been widely used in regenerative endodontics. Recent studies recommend that a safe concentration of 3Mix is in the range of 0.39 µg/mL and 1 mg/mL because higher concentrations may limit tissue regeneration. The aim of this study was to determine the regenerative capacity of isolated human dental pulp cells (DPCs) and apical papilla cells (APCs) after a 7-day treatment with selected doses of 3Mix. METHODS: Primary human DPCs/APCs from the third passage were divided into control and experimental groups. In the control group, cells were cultured in regular complete media. In the experimental group, cells were cultured in complete media containing 0.39 µg/mL or 1 mg/mL of 3Mix for 7 days. After the treatment period, the media were changed, and the cells were further tested for proliferation and differentiation potential. For cell proliferation, a colorimetric qualification of 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide was used on days 1, 3, 5, and 7. For differentiation potential, a dentinogenic differentiation medium was added into treated cells and cultured for 7, 14, and 21 days. Results were analyzed using quantitative alizarin red S staining and real-time reverse-transcription polymerase chain reaction. RESULTS: After 7 days of treatment, 100% cell death was discovered in the 1-mg/mL 3Mix group. The proliferative capacity of 0.39 µg/mL 3Mix-treated DPCs and APCs was significantly lower than that of untreated cells at all time points (P < .05). Mineralized nodule formation was found both in the 3Mix-treated and control groups, but it was significantly less in the 3Mix-treated groups at 7, 14, and 21 days (P < .01). Quantitative reverse-transcription polymerase chain reaction showed no statistically significant difference (95% confidence interval) in bone sialoprotein, alkaline phosphatase, and dentin matrix protein 1 gene expression in either 3Mix-treated DPCs or APCs compared with control groups. CONCLUSIONS: One milligram per milliliter of 3Mix had strong toxicity to DPCs/APCs when applied for 7 days, whereas 0.39 µg/mL 3Mix showed no toxicity but still affected cell proliferation and mineralization potential. However, no differences in dentinogenic gene expressions were observed between the 3Mix-treated and untreated groups.