RESUMEN
INTRODUCTION: There are hardly any publications about the outcome of cardiac rehabilitation considering patients with an increased need for medical, nursing and therapeutic care. The aim of this study, which consecutively included n=387 statutory health insurance inpatients over a period of 2 years, was to find out differences in outcome in self-care patients (Barthel index>70) as compared to patients with a need for complex care (Barthel index≤70). METHODS: Rehabilitation outcomes concerning physical capacity, emotional status and activities of daily living as measured by Barthel index, FIM index, HADS, clinical complications, exercise test, duration of rehabilitation and form of dismission were analyzed and compared between both groups. RESULTS: The inpatients with a Barthel index ≤70 at admission were older, had a longer stay in hospital and in rehabilitation, developed more complications and more often suffered from concomitant diseases. They were readmitted to hospital more often. They showed a comparatively higher increase in indices of self-care and a significant increase in physical performance tests. CONCLUSION: Higher medical care expenses of multimorbid cardiac inpatients are no contraindication against rehabilitation, because even in this group the specific rehabilitation aims of the healthcare payers can be reached.
Asunto(s)
Actividades Cotidianas/psicología , Rehabilitación Cardiaca/psicología , Rehabilitación Cardiaca/estadística & datos numéricos , Personas con Discapacidad/psicología , Personas con Discapacidad/rehabilitación , Autocuidado/estadística & datos numéricos , Anciano , Personas con Discapacidad/estadística & datos numéricos , Tolerancia al Ejercicio , Femenino , Alemania/epidemiología , Humanos , Tiempo de Internación/estadística & datos numéricos , Estudios Longitudinales , Masculino , Evaluación de Resultado en la Atención de Salud/métodos , Admisión del Paciente/estadística & datos numéricos , Prevalencia , Pronóstico , Estudios Retrospectivos , Autocuidado/psicología , Resultado del TratamientoRESUMEN
BACKGROUND: In some European countries telephone triage (TT) during out-of-hours primary care showed to be safe and effective. Other countries, such as Belgium, may not have trained auxiliary personnel while their national health services want to establish TT. OBJECTIVES: To compare urgency levels assessed by secretaries and general practitioners in one general practice cooperative in Belgium. METHODS: Percentage of correct-, under-, and over-triage were calculated in total and per reason for encounter. Inter-rater agreement was investigated. RESULTS: The secretaries correctly triaged (same urgency level) 77% of the telephone calls, under-triaged 10% and over-triaged 13%.'Shortness of breath', 'skin cuts', 'chest pain', 'feeling unwell' and 'syncope' were often under-triaged. CONCLUSION: Before introducing TT, auxiliary staff should be trained and protocols should be used.
Asunto(s)
Atención Posterior , Médicos Generales , Personal de Salud , Triaje , Bélgica , Humanos , TeléfonoRESUMEN
BACKGROUND: Distressing intrusive images are frequently experienced by sufferers from chronic and acute pain. The images (Index images) are correlated with elevations in anxiety, threat, and a cognition that the imaged event might actually happen. The over-estimation that having a negative cognition about an adverse event will increase the probability of the negative event occurring--the likelihood bias--has been observed in a variety of psychological disorders. Preliminary research indicated this cognitive bias might occur in pain sufferers. AIMS: To investigate the occurrence of a cognitive likelihood bias associated with imagery in acute and chronic pain sufferers, and to relate the postulated cognitive bias to psychological characteristics of participants, and four other important cognitive responses to their Index images. METHOD: Fifty-nine pain sufferers completed a newly developed questionnaire (Image-Event-Fusion-pain: IEF-p) to assess cognitive likelihood bias in pain sufferers. The internal consistency, reliability, factor structure and validity of the scale were evaluated. Psychological measures to assess anxiety, depression, PTSD symptoms, and levels of mental defeat were administered. RESULTS: The IEF-p was found to be psychometrically robust with satisfactory test-retest reliability, good internal consistency, single factor structure and criterion validity. The IEF-p was significantly correlated with four key cognitive appraisals of the Index Images (responsibility, likelihood, premonition, and threat). Three of these correlations were independent of depression. High cognitive bias scores were significantly associated with elevated levels of anxiety symptoms, depression, PTSD symptoms, and mental defeat. CONCLUSION: Pain Index images were significantly associated with cognitive bias (IEF-p), increased threat levels, and raised estimate of the likelihood of imaged events actually occurring. The results indicate the prevalence of a cognitive bias associated with pain imagery cognitions, comparable to that established with intrusive cognitions in OCD, notably Thought-Action- Fusion.
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Cognición , Imaginación , Dolor/psicología , Adulto , Femenino , Humanos , Funciones de Verosimilitud , Masculino , Persona de Mediana Edad , Dolor/rehabilitación , Psicometría , Encuestas y CuestionariosRESUMEN
BACKGROUND: The percentage of households that delays medical assistance due to financial reasons is slowly increasing. Moreover, some groups of the population do not ever find their way to primary health care and end up unnecessarily in the emergency department or with specialists. This study wants to examine how primary health care can be made accessible to these groups. AIM: In this study, we aim to discover whether in a city such as Antwerp primary health care is accessible to everyone. METHOD: The statistics were collected from the Health Care Survey done by the Welfare Services Antwerp in cooperation with the City of Antwerp. The questions were asked in three different ways: a postal questionnaire, a telephone questionnaire and a face-to-face interview. RESULTS: We determined that people who live on social welfare delay medical help due to financial reasons more frequently than the global Antwerp population. They often do not have a regular general practitioner (GP). Especially single parents, house-wives and house-husbands, job-seekers, incapacitated people unable to work, unskilled workers and foreigners are among the vulnerable groups where accessibility to primary health care is a concern. CONCLUSION: If we hope to improve the accessibility of primary health care, we must first and foremost inform the above-mentioned groups of the insurability and how this is applied. When this is fulfilled, it will be easier for the GP to receive this vulnerable group within the primary care system, so that the help of specialized care, which is often unnecessary, can be reduced.
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Accesibilidad a los Servicios de Salud/economía , Accesibilidad a los Servicios de Salud/estadística & datos numéricos , Atención Primaria de Salud/economía , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Bélgica/epidemiología , Estudios Transversales , Humanos , Persona de Mediana Edad , Aceptación de la Atención de Salud , Factores Socioeconómicos , Encuestas y Cuestionarios , Adulto JovenRESUMEN
INTRODUCTION: In many countries out of hours (OOH) care is offered by different health care services. General practitioners (GP) tend to offer services in competition with emergency departments (ED). Patients behaviour depends on a number of factors. In this study, we highlight the knowledge and ideas of patients concerning the co-payment system. METHODS: We used a mixed methods design, combining quantitative and qualitative research. During two weekends in January 2005, all patients using the ED or the GP OOH service, were invited for an interview with a structured questionnaire. A stratified random sample of patients participated in a semi-structured interview. Both methods add complementary data to answer the research questions. RESULTS: Most mentioned reasons for seeking help at the ED are: accessibility (15.0%), proximity (6.4%) and competence of the staff (5.6%). Reasons for choosing the GP are: GP is easy to find, minor medical problem or anxiety and confidence in the GP. The odds of not knowing the co-payment system are significantly higher in patients visiting the ED (OR 1.783; 95% CI: 1.493-2.129). Mostly GP users recognize the problem of ED overuse. They suggested especially to provide clear information about the tasks of the different services and about the payment system, to reduce ED overuse. CONCLUSION AND DISCUSSION: When intending to shift from ED to GP services for minor medical problems, aiming at just one measure is no option. Information campaigns aiming to address the entire population, can clarify the role of each player in out-of-hours care.
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Atención Posterior/estadística & datos numéricos , Servicios Médicos de Urgencia/estadística & datos numéricos , Conocimientos, Actitudes y Práctica en Salud , Atención Primaria de Salud/estadística & datos numéricos , Adolescente , Adulto , Atención Posterior/economía , Anciano , Anciano de 80 o más Años , Bélgica , Niño , Preescolar , Conducta de Elección , Servicios Médicos de Urgencia/economía , Femenino , Medicina General/estadística & datos numéricos , Accesibilidad a los Servicios de Salud , Mal Uso de los Servicios de Salud/economía , Mal Uso de los Servicios de Salud/estadística & datos numéricos , Humanos , Lactante , Recién Nacido , Masculino , Persona de Mediana Edad , Atención Primaria de Salud/economía , Investigación Cualitativa , Mecanismo de Reembolso/economía , Encuestas y Cuestionarios , Adulto JovenRESUMEN
Pulses from detectors used for radioactivity measurement can vary in size by several orders of magnitude. Large pulses will lead to saturation at the preamplifier output and extension of the pulse length. As a consequence, the dead time of the system increases and pulses may be lost. Electronic design techniques employed to protect against overloading tend to increase the amplifier noise level. However, an optoelectronic method of overload protection has been devised which has only a negligible effect on noise. An infrared light emitting diode interfaced to the output of the preamplifier is linked by fibre optic cable to an ultra-low leakage photodiode at the input. The conduction of the photodiode increases with the amplitude of the preamplifier output signal. Excess current is thereby prevented from entering the preamplifier and causing saturation. The preamplifier has been tested on 4π beta-gamma and gas counting systems and found to give good protection against overloading.
Asunto(s)
Amplificadores Electrónicos , Iluminación/instrumentación , Dispositivos Ópticos , Radiometría/instrumentación , Semiconductores , Diseño de Equipo , Análisis de Falla de Equipo , Dosis de RadiaciónRESUMEN
BACKGROUND: There is a dearth of information about imagery in pain sufferers. AIM: The aim of this study was to collect data on the characteristics, prevalence, and potency of imagery associated with pain. METHOD: The images of 59 pain sufferers were assessed by means of a semi-structured interview. The emotional, cognitive, behavioural, and pain-inducing properties (potency) of their index images were assessed by an image induction procedure and self-report scales of anxiety, depression and trauma symptoms. RESULTS: The results showed a remarkably high incidence of images in pain sufferers, with 78% of participants reporting one or more repetitive images when in pain. Exposure to their most powerful/distressing image (Index image) resulted in significant increases in negative emotions, negative cognitive appraisals, and in pain levels. In a sub-group of sufferers with significant levels of trauma symptoms, the index images elicited significantly higher levels of emotion and pain increment than did those respondents in a low/no trauma group. CONCLUSION: It was concluded that imagery is a prevalent, often "unobserved" but potent cognition in pain sufferers. The implications for CBT approaches to chronic pain, including image rescripting, are considered.
Asunto(s)
Dolor Crónico/psicología , Imágenes en Psicoterapia , Adulto , Trastornos de Ansiedad/diagnóstico , Trastornos de Ansiedad/psicología , Dolor Crónico/clasificación , Dolor Crónico/rehabilitación , Estudios Transversales , Cultura , Trastorno Depresivo/diagnóstico , Trastorno Depresivo/psicología , Femenino , Humanos , Entrevista Psicológica , Masculino , Persona de Mediana Edad , Terapia Ocupacional , Dimensión del Dolor/psicología , Proyectos Piloto , Centros de Rehabilitación , Rehabilitación Vocacional , Trastornos por Estrés Postraumático/diagnóstico , Trastornos por Estrés Postraumático/psicologíaRESUMEN
INTRODUCTION: In many countries, a reassessment of after-hours primary care has become necessary. In particular, centralised general practitioner deputizing services (GPDS) have emerged. In this study, consumers' preferences for after-hours medical care were obtained and the use of the new GPDS was predicted. METHOD: On the basis of the Theory of Reasoned Action, a survey was developed that was used at the Free Newborn and Child health care services in Antwerp. Consumers were asked about their knowledge, experience and perceptions concerning the performance of different medical services. RESULTS: 350 questionnaires were used for analysis. 98.6% of the respondents knew about the existence of the emergency department, whereas the GPDS was known by 81.7% of the respondents. The main reasons for preferring emergency department over the other services were an easy access, good explanation by the doctor and a late due time of the payment. Respondents preferred the GPDS mainly because of an expected shorter waiting time. Experience had a strong positive influence on choosing a particular after-hours medical service. CONCLUSION: In our study, the consumers' preferences concerning after-hours medical care were assessed. The following items are crucial for choosing after-hours care: experience with the services, easy access to the service, explanation by the doctor about the illness and the treatment and waiting time.
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Atención Posterior/normas , Conducta de Elección , Comportamiento del Consumidor , Adulto , Bélgica , Competencia Clínica , Femenino , Encuestas de Atención de la Salud , Accesibilidad a los Servicios de Salud , Humanos , Masculino , Listas de EsperaRESUMEN
Palliative chemotherapy for inoperable/metastatic oesophageal cancer has limited activity. This study assesses the feasibility and activity of gemcitabine and cisplatin in this group of patients. In total, 42 patients with locally advanced/metastatic squamous or adenocarcinoma of the oesophagus were treated with gemcitabine 1250 mg m(-2) days 1 and 8 and cisplatin 75 mg m(-2) day 1 in a 21-day cycle. Interim safety analysis was carried out after the first 19 patients suggested significant toxicity. The dose of gemcitabine was subsequently reduced to 1000 mg m(-2). Patients were assessed for toxicity and response. The median number of treatment cycles per patient was 4 (range 1-6). Grade 3-4 neutropenia occurred in 37% of cycles; however, there was only one episode of neutropenic fever. Nonhaematological toxicities included fatigue, nausea and vomiting. Among 32 patients eligible for response, there were three complete responses and 16 partial responses (overall response rate of 45%); nine patients had stable disease. Median survival was 11 months. The response rate appears to be greatest in those with squamous carcinoma compared to adenocarcinoma (71 vs 33%, P=0.036). The combination of gemcitabine and cisplatin in this schedule has manageable toxicity and significant activity in patients with locally advanced/metastatic oesophageal cancer and is worthy of further study.
Asunto(s)
Cisplatino/uso terapéutico , Desoxicitidina/análogos & derivados , Neoplasias Esofágicas/tratamiento farmacológico , Neoplasias Esofágicas/patología , Metástasis de la Neoplasia/patología , Adulto , Anciano , Cisplatino/efectos adversos , Desoxicitidina/efectos adversos , Desoxicitidina/uso terapéutico , Quimioterapia Combinada , Femenino , Humanos , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia/tratamiento farmacológico , Estadificación de Neoplasias , Tasa de Supervivencia , GemcitabinaRESUMEN
BACKGROUND: Numerous residents of Shelby County, Alabama, were infected with Salmonella when a restaurant unknowingly served food tainted with the bacterium. Because of the similarity in symptoms caused by other gastrointestinal pathogens and the variability in time of presentation, an outbreak such as this could be confused with one of another pathogenic origin. The pathogen identified, Salmonella bredeney, is a particularly rare cause of food poisoning. It makes up only 0.1% of the Salmonella isolates identified by the Centers for Disease Control and Prevention (CDC) each year. METHODS: We analyzed patient presentations through chart review and combined this information with that obtained from the state laboratories in Montgomery and the Shelby County Health Department. RESULTS: Symptoms were mostly gastrointestinal and ranged greatly in severity. The total number of patients affected in this incident exceeded 170, making it the largest epidemic of its kind in the recent history of Alabama. CONCLUSIONS: The outbreak in Shelby County was caused by an exceedingly rare species of Salmonella. At this time, it is the only outbreak of S bredeney reported in MEDLINE-accessible literature since 1983.
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Brotes de Enfermedades , Enfermedades Gastrointestinales/microbiología , Intoxicación Alimentaria por Salmonella/epidemiología , Intoxicación Alimentaria por Salmonella/microbiología , Salmonella/clasificación , Adulto , Anciano , Alabama/epidemiología , Antibacterianos/uso terapéutico , Niño , Enfermedades Gastrointestinales/tratamiento farmacológico , Enfermedades Gastrointestinales/epidemiología , Humanos , Persona de Mediana Edad , Intoxicación Alimentaria por Salmonella/tratamiento farmacológico , SerotipificaciónRESUMEN
Pregnant ewes were infected in midpregnancy with three isolates of Chlamydia pecorum derived from the feces of healthy lambs from three different farms. Oral infection, alone or together with Fasciola hepatica, did not result in tissue invasion, since all placental and fecal samples were negative for chlamydiae. Intravenous infection resulted in placental infection in 16 of 18 ewes in that chlamydiae were cultured from placentas or vaginal swabs. Two ewes bore dead lambs after a shortened gestation time. The chlamydiae isolated were all C. pecorum. There were no significant differences between the weights of the lambs from the infected groups and those from uninfected control ewes. Most ewes showed no serological evidence of infection by the complement fixation test; therefore, it is unlikely that the enteric subtype of C. pecorum is responsible for the cross-reactions sometimes seen in flocks being tested for C. psittaci infection.
Asunto(s)
Infecciones por Chlamydia/veterinaria , Complicaciones Infecciosas del Embarazo/veterinaria , Enfermedades de las Ovejas/microbiología , Animales , Chlamydia/clasificación , Chlamydia/patogenicidad , Infecciones por Chlamydia/complicaciones , Infecciones por Chlamydia/etiología , Fascioliasis/complicaciones , Heces/microbiología , Femenino , Genitales Femeninos/microbiología , Intestinos/microbiología , Placenta/microbiología , Embarazo , Complicaciones Infecciosas del Embarazo/etiología , Complicaciones Parasitarias del Embarazo , Ovinos , Enfermedades de las Ovejas/etiologíaRESUMEN
Faeces samples were taken per rectum from sheep on 26 farms in England and Wales and examined for the presence of chlamydia by culture in McCoy cell monolayers. Thirteen of the farms were known to have had abortion outbreaks associated with Chlamydia psittaci (enzootic abortion) and 13 were free of this infection. The chlamydia isolated were characterized by cultural techniques. Chlamydia were isolated from the faeces of lambs on all 26 farms and the prevalence of infection varied form 5-50% on individual farms. There was no significant difference between the proportion of infected lambs on farms where enzootic abortion was present or absent. Lambs first showed infection when they were 3 months old and the prevalence rate of infection increased up to 9 months old. No chlamydia were isolated from the faeces of 316 adult ewes. The chlamydia were identified as enteric rather than abortion type and thus were C. pecorum rather than C. psittaci.
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Infecciones por Chlamydia/veterinaria , Chlamydia/aislamiento & purificación , Heces/microbiología , Enfermedades de las Ovejas/diagnóstico , Aborto Veterinario/epidemiología , Aborto Veterinario/microbiología , Animales , Chlamydia/clasificación , Infecciones por Chlamydia/epidemiología , Infecciones por Chlamydia/microbiología , Chlamydophila psittaci/aislamiento & purificación , Femenino , Embarazo , Prevalencia , Estaciones del Año , Ovinos , Enfermedades de las Ovejas/epidemiología , Enfermedades de las Ovejas/microbiología , Especificidad de la Especie , Reino Unido/epidemiologíaRESUMEN
Analysis of the genomic DNAs of chlamydial isolates from sheep, cattle, and pigs was performed by Southern blot hybridization and by restriction endonuclease (RE) profiling of DNA amplified by PCR. The hybridization probes were derived from whole genomic DNA, the major outer membrane protein (MOMP) gene, the 16S rRNA gene, and an avian Chlamydia psittaci isolate plasmid. The PCR analysis used targets in the MOMP gene, the 16S rRNA gene, and the 60-kDa cysteine-rich protein gene. Together, the results showed that although there was considerable heterogeneity in the DNA sequence in the MOMP gene region, all the isolates had the same underlying total genomic RE profiles and yielded identical RE profiles for the rRNA and 60-kDa-protein gene regions. Most of the isolates were found to hybridize with the plasmid probe. Comparison of the MOMP sequence of one of the isolates (P787) with that of a known Chlamydia pecorum strain together with the results of the RE analyses allowed the conclusion that the isolates should all be classified within this new species.
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Enfermedades de los Bovinos/microbiología , Infecciones por Chlamydia/veterinaria , Chlamydia/genética , Genoma Bacteriano , Enfermedades de las Ovejas/microbiología , Enfermedades de los Porcinos/microbiología , Secuencia de Aminoácidos , Animales , Artritis Infecciosa/microbiología , Artritis Infecciosa/veterinaria , Secuencia de Bases , Southern Blotting , Bovinos , Infecciones por Chlamydia/microbiología , ADN Bacteriano/genética , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Reacción en Cadena de la Polimerasa , Rumiantes/microbiología , Ovinos , PorcinosRESUMEN
Some isolates of Chlamydia pecorum from sheep feces failed to produce inclusions on passage in cycloheximide-treated monolayers, but chlamydiae could be recovered several weeks later. Chlamydia psittaci from sheep abortions did not show this phenomenon.
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Chlamydia/fisiología , Cicloheximida/farmacología , Animales , Células Cultivadas , Heces/microbiología , Ratones , OvinosRESUMEN
We describe studies in whole kidney, cortical and medullary homogenates and, glomerular cells in culture to determine the relative levels of expression of alpha (Ya, Yc, Yk), mu (Yb1/Yb2), pi (Yf) glutathione S-transferases (GST) and CuZn superoxide dismutase (CuZn SOD) in different regions of the nephron. Immunoblotting and immunohistochemistry were used to demonstrate relatively weak expression of alpha, mu GST and, CuZn SOD in the glomerulus compared to that in particularly distal tubules. Whilst expression of Ya was found within glomerular cells, Yc, Yk and Yf were not detected. Immunofluorescence showed that Ya and Yb1/Yb2 but not Yf were expressed in cultured epithelial and mesangial cells studied between passages 1 and 3. While Ya was distributed in cytosol, Yb1/Yb2 was primarily located in nuclei.
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Glutatión Transferasa/análisis , Riñón/enzimología , Superóxido Dismutasa/análisis , Animales , Células Cultivadas/enzimología , Técnica del Anticuerpo Fluorescente , Glutatión Transferasa/clasificación , Immunoblotting , Corteza Renal/enzimología , Médula Renal/enzimología , Nefronas/enzimología , Ratas , Ratas Endogámicas WKYRESUMEN
Abortion and enteric isolates of Chlamydia psittaci from sheep differed in their growth in a fibroblastic cell culture derived from the small intestine of a lamb. Twenty abortion isolates, each from a different farm, produced large inclusions which could be passaged several times whereas 10 enteric isolates each from different farms (but from some of the farms of origin of the abortion isolates) produced sparse inclusions which could not be passaged. This appears to be a rapid method of distinguishing abortion and enteric isolates and may indicate different nutritional requirements or be related to the invasiveness of the isolates.
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Chlamydophila psittaci/crecimiento & desarrollo , Fibroblastos/microbiología , Ovinos/microbiología , Aborto Veterinario/microbiología , Animales , Medios de Cultivo , Duodeno/microbiología , Femenino , Embarazo , Enfermedades de las Ovejas/microbiologíaRESUMEN
This is a preliminary investigation into a recently defined urological disorder occurring in a subgroup of women with "urethral syndrome" suggestive of pelvic floor muscular (PFM) dysfunction. Symptoms include straining to void, urgency, frequency, hesitation, incontinence and/or retention, and subpubic pain. Finding neither bladder nor urological abnormalities, urologists may consider these women emotionally unstable without organic cause for their symptoms. However, their distress may be a consequence rather than a cause of their voiding problems. Sixteen female urological patients were matched with 16 asymptomatic controls to investigate PFM functioning, psychological status, and symptomatology. Results showed heterogeneity of symptomatology and little elevation of depression or anxiety when comparing patients with controls. Hypotheses of muscular abnormality were confirmed. Patients evidence poor control over testing and relaxing PFM, elevations of PFM activity under various conditions, and chronic pain as a prominent symptom. Treatment approaches specifically designed to address PFM dysfunction are discussed.
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Músculos/fisiopatología , Trastornos Psicofisiológicos/fisiopatología , Enfermedades Uretrales/fisiopatología , Urodinámica/fisiología , Adulto , Nivel de Alerta/fisiología , Enfermedad Crónica , Electromiografía , Femenino , Humanos , Relajación Muscular/fisiología , Dolor/fisiopatología , Dolor/psicología , Trastornos Psicofisiológicos/psicología , Síndrome , Enfermedades Uretrales/psicologíaRESUMEN
Twenty women diagnosed with functional urinary incoordination were randomly assigned to one of two treatment groups: biofeedback or progressive muscle relaxation. Ten subjects who were placed on a waiting list prior to treatment allocation served as a comparison group. The biofeedback intervention focused specifically on retraining of pelvic floor musculature (PFM). Patients were assessed pretreatment, posttreatment, and at 2-month follow-up. Outcome measures included self-reported symptomatology, psychological functioning, psychophysiological assessment of the PFM, and urologist ratings of problem severity and treatment efficacy. Both treatment approaches proved effective in improving symptomatology and psychological state. Subjects on the waiting list demonstrated no change in urological difficulties. No differences were found between the two treatment groups on any of the outcome measures. Theoretical and practical implications of the results are discussed.
Asunto(s)
Biorretroalimentación Psicológica , Relajación Muscular/fisiología , Trastornos Urinarios/terapia , Adulto , Nivel de Alerta/fisiología , Biorretroalimentación Psicológica/fisiología , Electromiografía , Femenino , Humanos , Dimensión del Dolor , Trastornos Urinarios/fisiopatología , Urodinámica/fisiologíaRESUMEN
Ewe placental and lamb intestinal isolates of Chlamydia psittaci recovered from flocks affected with ovine enzootic abortion were examined by inclusion morphology, indirect immunofluorescence (IIF) and immunoblot analysis. Chlamydiae recovered from the faeces of sheep from two flocks free of clinical disease were also examined. In cell culture ovine abortion (OA) and intestinal isolates were distinguishable by inclusion development and morphology. Similarly, in two-way IIF tests with one week mouse antisera isolates fell into two distinct groups: abortion or intestinal. Immunoblotting with convalescent sheep abortion antiserum identified 30 out of at least 40 silver staining polypeptides as antigenic both in OA and intestinal isolates. The serum produced a similar reaction pattern to the resolved proteins of each OA isolate, indicating a higher degree of antigenic conservation among these isolates. Considerable cross reactivity between the OA and intestinal isolates was identified, but the serum also showed apparent molecular weight differences between antigens of the two types in the 87-116 kDa, 38-44 kDa and 26-28 kDa regions. Furthermore, the immunoblotting analysis revealed heterogeneity among the intestinal isolates, particularly in antigens between 87-116 kDa and 38-44 kDa.