RESUMEN
This paper discusses the results of an experimental study on the effect of biphase calcium phosphate ceramics (BCPC) on laboratory-isolated polyresistant Gram-negative bacteria. Monitoring of this effect in a dynamical regimen was carried out upon Enterobacter cloacae 313, Klebsiella pneumoniae 227, Serratia marcescens 206, Klebsiella oxytoca 202, as well as on the standard strain Klebsiella pneumoniae 52145 (Institute Pasteur, Paris). The results show a significant antimicrobial effect of the ceramics. Antimicrobial properties are manifested during direct contact with BCPC and these depend on the quantity and grain size of the particles, as well as on the microbiological characteristics of the test microorganisms, and particularly on their cell size.
Asunto(s)
Sustitutos de Huesos/farmacología , Fosfatos de Calcio/farmacología , Cerámica/farmacología , Farmacorresistencia Microbiana , Bacterias Gramnegativas/efectos de los fármacos , Enterobacter cloacae/efectos de los fármacos , Klebsiella/efectos de los fármacos , Klebsiella pneumoniae/efectos de los fármacos , Tamaño de la Partícula , Serratia marcescens/efectos de los fármacosRESUMEN
An in vitro study of the influence of Biphase Calcium Phosphate Ceramics (BCPCs) on bacterial strains is presented. The test procedures were carried out in saline test solution to which different quantities (2,4,50,100, and 200 mg) of BCPCs(samples A and B) were added. The influence of BCPCs on standard bacterial strains has been determined by measuring bacterial contamination using the plate count method. The testing method was validated with membrane filtration and direct inoculation in culture media of sediment and supernatant. The test demonstrated reduction of the bacterial cell population in from 1 to 24 h in all experiments. The antibacterial effect revealed the specific inherent properties of the BCPCs under investigation.