RESUMEN
Intracellular dialysis and membrane voltage clamping were used to show that He-Ne laser irradiation of a pond snail neuron at a dose of 0.7 x 10(-4) J (power density 1.5 x 10(2) W/m2) increases the amplitude of the potential-dependent slow potassium current, while a dose of 0.7 x 10(-3) J decreases this current. Bupivacaine suppresses the potassium current. Combined application of laser irradiation at a dose of 0.7 x 10(-3) J increased the blocking effect of 10 microM bupivacaine on the slow potassium current, while an irradiation dose of 0.7 x 10(-4) J weakened the effect of bupivacaine.
Asunto(s)
Rayos Láser , Lymnaea/fisiología , Potenciales de la Membrana/fisiología , Neuronas/fisiología , Canales de Potasio/fisiología , Anestésicos Locales/farmacología , Animales , Bupivacaína/farmacología , Ganglios de Invertebrados/citología , Ganglios de Invertebrados/efectos de los fármacos , Ganglios de Invertebrados/efectos de la radiación , Helio , Terapia por Luz de Baja Intensidad , Lymnaea/citología , Lymnaea/efectos de los fármacos , Lymnaea/efectos de la radiación , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/efectos de la radiación , Neón , Neuronas/efectos de los fármacos , Neuronas/efectos de la radiación , Técnicas de Placa-Clamp , Canales de Potasio/efectos de los fármacos , Canales de Potasio/efectos de la radiaciónRESUMEN
Using clamp method it had been shown that He-Ne laser irradiation of the snail neurons increases the amplitude of voltage-gated slow potassium currents in dose of 0.7 x 10(-4) (fluence 1.5 x 10(2) Wt/m2) and decreases it in dose 0.7 x 10(-3). Bupivacaine and lidocaine suppressed potassium currents. Laser irradiation in dose 0.7 x 10(-3) enhanced the inhibitory effect of bupivacaine (10 mcM) and in dose 0.7 x 10(-4) it decreased the inhibitory effect of bupivacaine. The results of the study suggest mechanisms of the He-Ne laser irradiation effect in combination with pharmacological substances on ion channels of electrically excitable cells.