RESUMEN

Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide the sample template in a loop-mediated isothermal amplification (LAMP) reaction. This method is 10,000 times more sensitive than conventional PCR, costs under a dollar per sample, and can be completed from sampling to readout in just over half an hour.

Asunto(s)

ADN Viral/análisis , Geminiviridae/aislamiento & purificación , Técnicas de Amplificación de Ácido Nucleico , Enfermedades de las Plantas/virología , Vitis/virología , Granjas , Geminiviridae/clasificación , Geminiviridae/genética , Hojas de la Planta/virología , Reacción en Cadena de la Polimerasa , Sensibilidad y Especificidad