RESUMEN

Abstract Objectives: This study investigated the physical and mechanical properties, antibacterial effect and biocompatibility of novel elastomeric temporary resin-based filling materials (TFMs) containing zinc methacrylate (ZM). Material and Methods: Experimental TFMs were prepared by mixing the zinc methacrylate with monomer, co-monomer, photoinitiator and fillers. A ZM concentration of 0 (control), 0.5% (Z0.5); 1% (Z1), 2% (Z2), or 5% (ZM5) wt% was added to the TFMs. Fermit-N (F) was used for comparison with the experimental material. Microleakage, water sorption/solubility, degree of conversion, depth of cure, ultimate tensile strength, and hardness were determined and compared. A modified direct contact test (DCT) with Enterococcus faecalis and a Streptococcus mutans' biofilm accumulation assay was carried out to evaluate the antimicrobial effect and cytotoxicity of the assay. Statistical comparisons were performed (α=5%). Results: The results showed that the physical and mechanical properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference and some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values. TFMs with ZM killed E. faecalis only after 1 h. Biofilm development of S. mutans was not affected by the inclusion of ZM in the experimental TFMs. Conclusions: The present findings suggest that the physical, mechanical and biological properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference. However, some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values.

Asunto(s)

Animales , Bovinos , Zinc/química , Resinas Compuestas/química , Elastómeros/química , Restauración Dental Provisional/métodos , Metacrilatos/química , Valores de Referencia , Solubilidad , Streptococcus mutans/efectos de los fármacos , Resistencia a la Tracción , Factores de Tiempo , Zinc/farmacología , Ensayo de Materiales , Recuento de Colonia Microbiana , Distribución Aleatoria , Reproducibilidad de los Resultados , Enterococcus faecalis/efectos de los fármacos , Resinas Compuestas/farmacología , Elastómeros/farmacología , Filtración Dental , Pruebas de Dureza , Metacrilatos/farmacología , Antibacterianos/farmacología

RESUMEN

OBJECTIVES: This study investigated the physical and mechanical properties, antibacterial effect and biocompatibility of novel elastomeric temporary resin-based filling materials (TFMs) containing zinc methacrylate (ZM). MATERIAL AND METHODS: Experimental TFMs were prepared by mixing the zinc methacrylate with monomer, co-monomer, photoinitiator and fillers. A ZM concentration of 0 (control), 0.5% (Z0.5); 1% (Z1), 2% (Z2), or 5% (ZM5) wt% was added to the TFMs. Fermit-N (F) was used for comparison with the experimental material. Microleakage, water sorption/solubility, degree of conversion, depth of cure, ultimate tensile strength, and hardness were determined and compared. A modified direct contact test (DCT) with Enterococcus faecalis and a Streptococcus mutans' biofilm accumulation assay was carried out to evaluate the antimicrobial effect and cytotoxicity of the assay. Statistical comparisons were performed (α=5%). RESULTS: The results showed that the physical and mechanical properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference and some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values. TFMs with ZM killed E. faecalis only after 1 h. Biofilm development of S. mutans was not affected by the inclusion of ZM in the experimental TFMs. CONCLUSIONS: The present findings suggest that the physical, mechanical and biological properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference. However, some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values.

Asunto(s)

Resinas Compuestas/química , Restauración Dental Provisional/métodos , Elastómeros/química , Metacrilatos/química , Zinc/química , Animales , Antibacterianos/farmacología , Bovinos , Recuento de Colonia Microbiana , Resinas Compuestas/farmacología , Filtración Dental , Elastómeros/farmacología , Enterococcus faecalis/efectos de los fármacos , Pruebas de Dureza , Ensayo de Materiales , Metacrilatos/farmacología , Distribución Aleatoria , Valores de Referencia , Reproducibilidad de los Resultados , Solubilidad , Streptococcus mutans/efectos de los fármacos , Resistencia a la Tracción , Factores de Tiempo , Zinc/farmacología

RESUMEN

The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic.

Asunto(s)

Antibacterianos/química , Antibacterianos/farmacología , Resinas Compuestas/química , Resinas Compuestas/farmacología , Enterococcus faecalis/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Streptococcus mutans/efectos de los fármacos , Animales , Bisfenol A Glicidil Metacrilato/química , Bisfenol A Glicidil Metacrilato/farmacología , Bovinos , Supervivencia Celular/efectos de los fármacos , Filtración Dental , Restauración Dental Provisional/métodos , Cementos de Ionómero Vítreo/química , Cementos de Ionómero Vítreo/farmacología , Pruebas de Dureza , Ensayo de Materiales , Metilmetacrilatos/química , Metilmetacrilatos/farmacología , Ratones , Ácidos Polimetacrílicos/química , Ácidos Polimetacrílicos/farmacología , Reproducibilidad de los Resultados , Materiales de Obturación del Conducto Radicular/química , Materiales de Obturación del Conducto Radicular/farmacología , Solubilidad , Resistencia a la Tracción , Factores de Tiempo , Cemento de Óxido de Zinc-Eugenol/química , Cemento de Óxido de Zinc-Eugenol/farmacología

RESUMEN

Abstract The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic.

Asunto(s)

Animales , Bovinos , Ratones , Streptococcus mutans/efectos de los fármacos , Enterococcus faecalis/efectos de los fármacos , Resinas Compuestas/farmacología , Resinas Compuestas/química , Fibroblastos/efectos de los fármacos , Antibacterianos/sangre , Antibacterianos/farmacología , Ácidos Polimetacrílicos/farmacología , Ácidos Polimetacrílicos/química , Materiales de Obturación del Conducto Radicular/farmacología , Materiales de Obturación del Conducto Radicular/química , Solubilidad , Resistencia a la Tracción , Factores de Tiempo , Cemento de Óxido de Zinc-Eugenol/farmacología , Cemento de Óxido de Zinc-Eugenol/química , Ensayo de Materiales , Supervivencia Celular/efectos de los fármacos , Reproducibilidad de los Resultados , Bisfenol A Glicidil Metacrilato/farmacología , Bisfenol A Glicidil Metacrilato/química , Restauración Dental Provisional/métodos , Cementos de Ionómero Vítreo/farmacología , Cementos de Ionómero Vítreo/química , Pruebas de Dureza , Metilmetacrilatos/farmacología , Metilmetacrilatos/química

RESUMEN

INTRODUCTION: The aim of this study was to evaluate the physical properties, the antimicrobial effect, and the biocompatibility of dual polymerization experimental sealers after the incorporation of dibutyltin (ET) or calcium (EC) methacrylate at concentrations of 0.5%, 1%, 2%, and 5%. METHODS: RealSeal (RS; SybronEndo, Glendora, CA) was used as a commercial control. Materials were evaluated regarding film thickness, degree of conversion, radiopacity, antimicrobial effect against Enterococcus faecalis using the modified direct contact test, and cell viability. Data were analyzed using analysis of variance followed by the Tukey test or the Student-Newman-Keuls test in SigmaPlot 12.0 (Systat Software, Inc, Point Richmond, CA) (P = .05). RESULTS: The film thickness of the dibutyltin and calcium were greater than experimental sealers following the standards given by ISO 6876:2012. For degree of conversion, dual polymerization was not influenced by the addition of metal methacrylate. Regarding the modified direct contact test, calcium and dibutyltin at all concentrations showed antimicrobial activity when compared with the positive control after 48 hours of contact (P < .05). In cell viability, ET at all concentrations showed high cytotoxicity similar to RS, and EC at concentrations of 0.5%, 1%, and 2% showed moderate cytotoxicity that was less than 5% of calcium and RS. CONCLUSIONS: It was concluded that calcium and dibutyltin methacrylate incorporation in experimental sealers promoted the antimicrobial effect. The incorporation of calcium methacrylate at 0.5%, 1%, and 2% seemed to be a good treatment option to provide antimicrobial activity associated with moderate cytotoxicity and adequate physical properties.

Asunto(s)

Antiinfecciosos/farmacología , Metacrilatos/farmacología , Materiales de Obturación del Conducto Radicular/farmacología , Antiinfecciosos/química , Calcio , Resinas Compuestas/química , Resinas Compuestas/farmacología , Ensayo de Materiales , Metacrilatos/química , Compuestos Orgánicos de Estaño , Materiales de Obturación del Conducto Radicular/química

RESUMEN

OBJECTIVE: To determine the color stability (CS) and hardness' decrease (HD) of two commercial resin-based composites (RBCs), after in situ staining conditions (SC). METHODS: Six disc-shaped specimens (6 × 2 mm) of two RBCs-Filtek Z350 XT (FZX) and IPS Empress Direct (IED)-were randomly distributed into palatal devices. Eight volunteers (aged 20-30 years) participated in this study. The in situ SC consisted of 400 mL consumption (2 × 200 mL/day) of water, orange juice, cola soda, and coffee and 140 mL consumption of red wine, 10 days each. CS was obtained by CIELAB color difference (ΔE*) and HD (in %) by the Knoop method (50 g for 15 s), after curing (baseline) and after the in situ SC. Data were statistically analyzed at α = 0.05 significance level. RESULTS: For both RBCs, the coffee (5.7 ± 0.5 for FZX; 5.9 ± 1.3 for IED) and red wine (6.1 ± 1.1 for FZX; 6.2 ± 1.6 for IED) groups showed the highest ΔE* while water the lowest (1.6 ± 0.6 for FZX; 1.8 ± 0.2 for IED). The highest HD was induced by red wine group for FZX (40.7 ± 5.4) and by cola soda for IED (40.6 ± 3.2) while the water groups (8.3 ± 1.7 for FZX; 7.8 ± 2.1 for IED) presented the lowest values for both RBCs. CONCLUSION: Both RBCs were affected by the in situ SC, but no significant differences for CS and HD were observed between them. Red wine and coffee showed higher discoloration potential. CLINICAL SIGNIFICANCE: This in situ study suggests that color stability and hardness' decrease of resin-based composites were dependent on the type of consumed beverage and materials' composition. Patients with esthetic direct restorations should be aware of the degradative potential of them.

Asunto(s)

Color , Resinas Compuestas/química , Adolescente , Silicatos de Aluminio , Bebidas Gaseosas , Citrus , Café , Colorimetría , Materiales Dentales/química , Porcelana Dental , Estética Dental , Femenino , Dureza , Humanos , Masculino , Ensayo de Materiales , Propiedades de Superficie , Vino

RESUMEN

The pursuit of esthetic excellence in dentistry today requires dental practitioners to enhance their knowledge and technique in restorations of the anterior teeth. Due to the high incidence and prevalence of fractures in this dental area, mimicking the optical properties and characteristics of the dental structure in young patients (aged 6 to 15 years) is a major challenge. This case report describes the minimally invasive, non-beveled restoration of two fractured anterior teeth (class IV). After diagnosing fractures of teeth 11 and 21 in a 10-year-old patient, a composite mock-up was performed, followed by a diagnostic wax-up. With the aid of a silicon guide, the restorations were prepared without beveling the enamel surface. We performed color stratification by using different types of composite resins. Finally, finishing and polishing procedures were carried out.

---

A busca da excelência estética na odontologia atual exige, por parte do cirurgião-dentista, o aprimoramento de seus conhecimentos e técnicas para a confecção de restaurações em dentes anteriores. Em razão da alta incidência e prevalência de fraturas nesta região dentária, o desafio de mimetizar as propriedades e características ópticas da estrutura dental em pacientes jovens (entre 6 e 15 anos) torna-se um desafio. O presente caso clínico relata a restauração minimamente invasiva, sem utilização de bisel, na superfície dentária de duas classes IV. Após a constatação de fratura nos elementos 11 e 21 do paciente com 10 anos, realizou-se o ensaio restaurador do mesmo, com posterior moldagem e enceramento diagnóstico. A partir da confecção do guia de silicone, confeccionaram-se as restaurações através da técnica da muralha e posteriormente foi realizada a estratificação de cores mediante o uso de diferentes tipos de resina composta. Finalmente, realizaram-se os procedimentos de acabamento e polimento.