RESUMEN
In order to examine the impact of Cu(II)-Cr(VI) co-pollution in printing and dyeing wastewater on the aniline biodegradation system (ABS), loading experiments were conducted on ABS at varying concentrations of Cu(II)-Cr(VI). The synergistic stress imposed by Cu(II)-Cr(VI) accelerated the deterioration of the systems, with only the C2-3 (2 mg/L Cr(VI)-3 mg/L Cu(II)) sustaining stable operation for 42 days. However, its nitrogen removal performance remained significantly impaired, resulting in a total nitrogen (TN) removal rate below 40%. High-throughput sequencing analysis revealed a stronger correlation between Cr(VI) and microbial diversity compared to Cu(II). Metagenomic sequencing results demonstrated that Cu(II) emerged as the dominant factor influencing the distribution of dominant bacteria in C2-3, as well as its contribution to contaminant degradation. The complex co-pollution systems hindered aniline degradation and nitrogen metabolism through the combined bio-toxicity of heavy metals and aniline, thereby disrupting the transport chain within the systems matrix.
Asunto(s)
Metales Pesados , Metales Pesados/metabolismo , Cromo/metabolismo , Redes y Vías Metabólicas , NitrógenoRESUMEN
In order to explore the stress principle of Cr (â ¥) on aniline biodegradation system, a control group and experimental groups with the concentration of Cr (â ¥) at 2, 5, 8 mg/L were set up. The results demonstrated that Cr (â ¥) had minimal effects on the degradation efficiency of aniline but significantly inhibited nitrogen removal function. When Cr (â ¥) concentration was below 5 mg/L, the nitrification performance recovered spontaneously, while denitrification performance was severely impaired. Furthermore, the secretion of extracellular polymeric substances (EPS) and its fluorescence substance concentration were strongly inhibited with increasing Cr (â ¥) concentration. High-throughput sequencing revealed that the experimental groups were enriched with Leucobacter and Cr (â ¥)-reducing bacteria, but the abundance of nitrifiers and denitrifiers was significantly decreased compared to the control group. Overall, the effects of Cr (â ¥) stress at different concentrations on nitrogen removal performance were more significant than those on aniline degradation.
Asunto(s)
Desnitrificación , Aguas del Alcantarillado , Aguas del Alcantarillado/microbiología , Reactores Biológicos/microbiología , Nitrificación , Compuestos de Anilina/metabolismo , Nitrógeno/metabolismoRESUMEN
Aniline is a highly bio-toxic industrial product, even at low concentrations, whose related wastewater has been flowing out worldwide on a large scale along with human production. As a green technology, aerobic biological treatment has been widely applied in industrial wastewater and exhibited various characteristics in the field of aniline wastewater. Meanwhile, this technology has shown its potential of synchronous nitrogen removal, but it still consumes energy badly. In the face of resource scarcity, this review comprehensively discusses the existing research in aerobic biodegradation of aniline wastewater to find out the developmental dawn of aerobic biological treatment. Primarily, it put forward the evolution history details of aniline biodegradation from pure culture to mixed culture and then to simultaneous nitrogen removal. On this basis, it presented the existing challenges to further expand the application of aerobic biotechnology, including the confusions of aniline metabolic mechanism, the development of co-degradation of multiple pollutants and the lack of practical experience of bioreactor operation for aniline and nitrogen removal. Additionally, the prospects of the technological shift to meet the needs of an energy-conserving society was described according to existing experiences and feasibility. Including but not limiting to the development of multifunctional bacteria, the reduction of greenhouse gases and the combination of green technologies.
Asunto(s)
Aguas del Alcantarillado , Aguas Residuales , Humanos , Bacterias/metabolismo , Biodegradación Ambiental , Reactores Biológicos/microbiología , Compuestos de Anilina , Nitrógeno/metabolismoRESUMEN
In order to optimize the degradation of high-concentration aniline wastewater, the operation of sequencing batch bioaugmentation reactors with different aniline concentrations (200 mg/L, 600 mg/L, 1000 mg/L) was studied. The results showed that the removal rates of aniline and COD in the three reactors could reach 100%. When the aniline increased to 600 mg/L, the nitrogen removal efficiency reached the peak (51.85%). The increase of aniline inhibited the nitrification, while denitrification was enhanced due to the increase of C/N ratio. But this change was reversed by the toxicity of high concentrations of aniline. The metagenomic analysis showed that when the aniline concentration was 600 mg/L, the abundance distribution of microbial samples was more uniform. The improved of aniline concentration had led to the increase of aromatic compounds degradation metabolic pathways. In addition, the abundance of aniline degradation and nitrogen metabolism genes (dmpB, xylE, norB) was also promoted.
Asunto(s)
Contaminantes Ambientales , Aguas Residuales , Desnitrificación , Aguas del Alcantarillado , Reactores Biológicos , Nitrificación , Nitrógeno/metabolismo , Compuestos de Anilina/metabolismo , Redes y Vías Metabólicas/genéticaRESUMEN
To evaluate the ecological risk of aniline wastewater biodegradation, the aniline wastewater (200 mg/L) was treated in this work under the stress of Cu (II) at 3, 6 and 10 mg/L, respectively. The slight fluctuation of aniline-degrading performance and the significant inhibition of nitrogen removal was caused by the Cu (II) stress at below 6 mg/L. Meanwhile, the tolerance of nitrifying performance to Cu (II) was higher than denitrifying. The collapse of biosystem was caused by the Cu (II) stress at 10 mg/L and the decontamination function was disabled within 8 days. The activity and stability of sludge declined under the increase of Cu (II) content. Microbial diversity results demonstrated that the genera with heavy-metal tolerance represented by Zoogloea and Azospira significantly dominated under the continuously Cu (II) stress. Whereas, the biosystem with these dominant genera did not achieve the comparable aniline and nitrogen removal performance as the control group.
Asunto(s)
Microbiota , Aguas del Alcantarillado , Compuestos de Anilina , Reactores Biológicos , Depresión , Nitrificación , Nitrógeno/análisis , Aguas ResidualesRESUMEN
To investigate the effect of residual coagulant after coagulation pretreatment on activated sludge system of wastewater treatment plants (WWTPs), comparative evaluation of lab-scale sequencing batch reactors under different poly-aluminum chloride (PAC) concentrations (20 and 55 mg/L), presenting the performance differences of reactors. Results showed that the PAC concentration of 20 mg/L slightly enhanced the average removal efficiencies of chemical oxygen demand (COD) and total nitrogen (TN), up to 93.43% and 72.52%. Whereas, an inhibition effect was exerted at the PAC concentration of 55 mg/L, the average removal efficiencies decreased to 88.56% and 57.80% respectively. Similarly, the residual aluminum salts showed a concentration effect of low promotion and high inhibition on sludge activity index. The content of specific oxygen utilization rate (SOUR) and dehydrogenase (DHA) sharply decreased by 30.17% and 53.56% under the high PAC concentration of 55 mg/L. Activity recovery phase showed that the suppression of aluminum salt coagulant on biological system was reversible. High-throughput sequencing presented that the relative abundance of microbes showed obvious variations at different PAC concentrations, and certain bacteria in Chloroflexi and Bacteroidota exhibited better adaptability to the high PAC concentration environment. Nevertheless, the antagonism action between denitrifying genera and other genera as well as the downregulation of functional enzymes regarding nitrogen metabolism gave rise to the deterioration of denitrification under the high PAC concentration of 55 mg/L. This study revealed the influence mechanism of residual aluminum salt coagulant on activated sludge system, providing strategies for efficient decontamination and long-term stable operation of biological system in wastewater treatment plant under the condition of adding PAC.
Asunto(s)
Microbiota , Aguas del Alcantarillado , Aluminio , Reactores Biológicos/microbiología , Nitrógeno/análisis , Aguas del Alcantarillado/química , Eliminación de Residuos Líquidos/métodosRESUMEN
Due to the inhibition of nitrification from aniline toxicity, exogenous N-acyl-homoserine lactones (AHLs) addition was attempted to enhance nitrogen removal in this work. Two sequencing batch biofilm reactors (SBBRs): S1 (the control) and S2 (C6-HSL and 3-oxo-C8-HSL dosing) were used to treat aniline wastewater. The NH4+-N and TN removal rates of S2 were 42.50% and 26.99% higher than S1 in the aerobic phase, respectively. It revealed the nitrogen removal performance of S2 much better than S1. High-throughput sequencing results indicated that many nitrifiers and denitrifiers of S2, such as Nitrosomonas and Thauera, transferred from sludge to biofilm significantly and built closer relationships each other. Overall, main nitrogen removal was contributed by biofilm rather than sludge with the regulation of AHLs. A mild and collaborative environment of biofilms for microorganisms enhanced nitrogen removal. The work provided a new idea for reconciling the contradiction between nitrification and denitrification in aniline wastewater treatment.
Asunto(s)
Acil-Butirolactonas , Microbiota , Compuestos de Anilina , Biopelículas , Reactores Biológicos , Desnitrificación , Nitrificación , Nitrógeno , Aguas del Alcantarillado , Aguas ResidualesRESUMEN
To break the contradiction between aniline and nitrogen metabolism in activated sludge reactor by influencing microbial interspecific communication, Auto-inducer C6-HSL and 3-oxo-C8-HSL were selected in this study to interfere with aniline degradation system. The two Auto-inducers enhanced the aniline degradation rate and ammonia removal efficiency of the systems, especially C6-HSL. Meanwhile, the main ammonia removal way was assimilation. Exogenous Auto-inducer effectively stabilized the sludge structure and activity from the destruction of aniline, and promoted EPS secretion. Microbial diversity analysis showed that most of functional microflora of seed sludge gradually deactivated with the operation of the reactor, while Rhodococcus, Leucobacter, g_norank_f_Saprospiraceae proliferated wildly under the action of Auto-inducer. Additionally, the interspecific relationship also demonstrated a different trend. Exogenous Auto-inducer was proved to exert positive effects on aniline degradation system to a certain extent, providing new insights in the field of aniline wastewater bio-degradation.
Asunto(s)
Microbiota , Aguas del Alcantarillado , Compuestos de Anilina , Reactores Biológicos , DescontaminaciónRESUMEN
The strategy of adjusting aeration time (5 h/6 h/7 h) was applied to the sequential batch reactors to optimize the treatment of aniline wastewater (600 mg/L) conveniently and economically. Three reactors degraded aniline effectively. The nitrogen removal ability of system with 6 h aeration time was better, performing the similar denitrification property as 5 h and nitrification performance as 7 h. Meanwhile, longer aeration time potentially damaged the sludge structure. The metagenomic analysis explained the micro-mechanism for the better performance of the system with 6 h aeration time. Appropriate aeration time was conducive to the enrichment of synergistic microflora, including aniline degrading-bacteria, heterotrophic nitrifiers and denitrifiers. Then, the tilt of environmental resources to these floras in the system was beneficial to the maximum value utilization of living substrates. Accordingly, these bacteria were more closely related to genes, resulting in higher expression of functional genes in the system.
Asunto(s)
Reactores Biológicos , Desnitrificación , Compuestos de Anilina , Nitrificación , Nitrógeno , Aguas del AlcantarilladoRESUMEN
Comparative evaluation of SBRs under different modes (AX/O, AN/AX/O, AN/O/AX, O/AX) with same aniline wastewater arrangements, presenting the startup and performance differences of reactors. The results revealed that the four systems realized the efficient aniline and NH4+-N removal on the basis of sufficient aerobic time. Anaerobic aniline degradation was also achieved in the first three reactors after acclimation. The denitrification efficiency was the highest in O/AX reactor and the lowest in AN/O/AX due to mode sequence setup. Pollutants variations in the typical cycles experimental data combined with microbial diversity analysis were highlighted that aerobic denitrification contributed the most under O/AX mode, while the other three modes relied on anoxic denitrification. Meanwhile, low nitrifiers and aerobic denitrifiers abundance might be another reason for the poor denitrification of AN/O/AX mode. It was inferred that denitrification was most susceptible to operation mode sequences.
Asunto(s)
Reactores Biológicos , Contaminantes Ambientales , Compuestos de Anilina , Desnitrificación , Nitrificación , Nitrógeno/análisis , Aguas del Alcantarillado , Aguas ResidualesRESUMEN
To cross nitrogen removal barrier, carbon sources (sodium succinate (Z1), sodium acetate (Z2) and glucose (Z3)) were applied in aniline degradation reactor to enrich heterotrophic nitrifiers and denitrifiers. The aniline was degraded almost completely and the nitrogen removal performance was improved in three systems. The total nitrogen (TN) removal efficiency of Z2 was the highest. The dominant bacteria were phylum Proteobacteria, class BetaProteobacteria, and genus Thauera (Z1, Z3), Leptothrix (Z2). Different aniline degrading bacteria, heterotrophic nitrifiers and denitrifiers were enriched, and Z2 had more high-abundance communities. Three systems followed the meta-cleavage pathway for the aniline degradation according to the genes annotation. Particularly, the contribution of each genus to nitrogen metabolism and aromatic compounds degradation in the Z2 was more evenly distributed, rather than relying mainly on the contribution of Thauera in Z1 and Z3 so that more functional genes related nitrogen metabolism and aniline degradation were more abundant in Z2.
Asunto(s)
Desnitrificación , Nitrógeno , Compuestos de Anilina , Reactores Biológicos , Carbono , Procesos HeterotróficosRESUMEN
An enhanced sequencing batch reactor (SBR) system was developed to treat synthetic wastewater rich in 600 mg/L aniline. The aniline degradation efficiency was almost 100%, and the total nitrogen (TN) removal rate was more than 50%. Metagenomics technology revealed the community structure, functional genes and metabolic mechanism during the start-up of the enhanced reactor. Sequencing results showed that Proteobacteria, Bacteroidetes, Chloroflexi and Actinobacteria were dominant phylum. The proportion of degradation of aromatic compounds function increased gradually, but the proportion of nitrogen metabolism function changed little. Functional genes involved in aniline degradation including benA-xylX and dmpB/xylE were detected. The functional genes of nitrogen metabolism were involved in complete nitrification, traditional denitrification, assimilation nitrate reduction and dissimilation nitrate reduction. The functional contribution analysis and network analysis showed that the cooperation and competition of Thauera, Delftia, Diaphorobacter, Micavibrio and Azoarcus ensured the effective removal of aniline and nitrogen.