RESUMEN
Comparative investigations were carried out on the antibody level in test animals--sheep, vaccinated with a polyvaccine against C and D enterotoxemia and necrotic hepatitis as well as in combined application with Levamisol. It was found that with the use of the preparation the stimulation of immunogenesis with type D and necrotic hepatitis--type B antigens (included in the composition of the polyvaccine) was enhanced. No stimulation of immunogenesis with the type C specific antigen was found within the period of investigation. Immunity was better built up when the animals were treated with the combined product as compared with the controls, given the polyvaccine without Levamisol.
Asunto(s)
Adyuvantes Inmunológicos , Vacunas Bacterianas/inmunología , Clostridium/inmunología , Inmunización/veterinaria , Levamisol/uso terapéutico , Ovinos/inmunología , Tetramisol/uso terapéutico , Animales , Anticuerpos Antibacterianos/análisis , Clostridium perfringens/inmunología , Combinación de Medicamentos , Evaluación de Medicamentos/veterinaria , Inmunización/métodosRESUMEN
A comparative study was carried out on the lethal properties of dry toxins of Clostridium oedematiens Type B obtained by means of salting cultural Seitz-filtrates, containing or lacking protease inhibitors, being dried for 24 and 48 hours at 37 degrees C. It was found that when ammonium sulfate was used for salting, the dry toxins from such filtrates, containing 0.0001 gram% specific soya inhibitor, showed 2 to 300 times higher toxicity than those containing no inhibitor. The addition of 3 per cent normal horse serum to the cultural filtrates of Clostridium oedematiens Type B favoured the production of dry toxins of higher lethal properties. The decrease in the lethal properties of a dry toxin without inhibitor was found to be in a positive correlation with the drying term and the residual moisture of the toxin. When a protease inhibitor was employed the drying term had no effect on the activity of the dry toxins.