RESUMEN
OBJECTIVES: Recent investigations which have aimed at unraveling the etiology of multiple sclerosis (MS), have underscored the role of mitochondria in this disorder. PINK1 gene codes a serine/threonine kinase that protects mitochondria and maintains its normal function. METHODS: In the current project, we quantified expression levels of PINK1 and a long non-coding RNA which is transcribed antisense to this gene (PINK1-AS) in the peripheral blood of MS patients versus normal persons. RESULTS: Peripheral expression of PINK1-AS was remarkably higher in MS patients compared with healthy individuals. A significant difference in PINK1-AS level was also recognized in male patients compared with male controls. But, the difference was not remarkable between female subgroups. Expression of PINK1 was not different between MS patients and healthy persons. Univariate analysis showed significant differences in age, disease duration, progression index and age at disease onset between males and females (P values of 0.041, 0.001, <0.0001 and 0.007 respectively). There was a trend toward correlation between expression levels of PINK1 and PINK1-AS (r = 0.26, P = 0.074). However, expressions of either genes were correlated with any of the demographic or clinical features. CONCLUSION: Based on the altered expression of PINK1-AS in the peripheral blood of MS patients, PINK1-AS might be a putative culpript in the pathogenesis of MS. We recommend conduction of additional studies to unravel the mechanism of PINK1-AS partake in the MS.
Asunto(s)
Regulación Enzimológica de la Expresión Génica , Esclerosis Múltiple/genética , Proteínas Quinasas/genética , ARN sin Sentido/genética , Adulto , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Caracteres SexualesRESUMEN
Expressions of the Growth arrest specific 8 (GAS8) and its naturally occurring anti-sense RNA (GAS8-AS1) have been assessed in tumoral tissues of different origins. However, their association with immune-related disorders has been poorly understood. In the current study, we evaluated expression levels of these genes in 50 relapsing-remitting multiple sclerosis (RRMS) patients compared with age- and sex-matched controls. Expressions of both genes were significantly higher in total MS patients compared with controls (P = 0.001 and P < 0.0001 respectively). The difference in GAS8 expression was also significant in total female patients and females aged less than 50 when compared with the corresponding control subjects (P = 0.002 and 0.006 respectively). GAS8-AS1 was higher in male patients in both age-based subgroups compared with the corresponding healthy subjects (P < 0.0001). Expressions of both genes were inversely correlated with age of male study participants but no other subgroups. GAS8-AS1 transcript levels had 99.6% accuracy in diagnosis of disease status in male subjects. The current study shows significance of GAS8 and GAS8-AS1 in the pathogenesis of MS and the putative role of GAS8-AS1 as a diagnostic biomarker in a subset of patients.