RESUMEN

Transfer of human papillomavirus type 11 (HPV11) DNA and a neo(r) marker into primary rat embryo cells (REC) led to colony formation in G418-selective medium. About 20% of HPV11 clones were eventually established in culture but displayed low growth rates. Cotransfection of HPV11 DNA and an activated ras oncogene led to formation of both drug-resistant flat colonies and phenotypically transformed clones which grew efficiently when expanded in culture. A number of transformants reverted to a flat, "normal" morphology shortly after isolation. Nontransformed clones expressed only HPV11 genes, while those maintaining a transformed phenotype transcribed both ras and HPV11 genes efficiently and were highly tumorigenic. Expression of HPV11 thus seems, necessary for induction of colony formation, but efficient long-term growth seems to require at least the transient presence of ras.

Asunto(s)

Transformación Celular Viral , ADN Viral/genética , Genes ras , Papillomaviridae/genética , Transfección , Animales , Antibacterianos/farmacología , Northern Blotting , Southern Blotting , División Celular , Células Clonales , ADN Viral/aislamiento & purificación , Resistencia a Medicamentos/genética , Embrión de Mamíferos , Femenino , Gentamicinas/farmacología , Humanos , Cinética , Neoplasias Experimentales/patología , Plásmidos , ARN/aislamiento & purificación , Ratas , Ratas Endogámicas F344