RESUMEN
The phenomenon of induction of antibodies to morphine in morphine-treated animals and opiate addicts was shown by means of ELISA. The reaction of the immune system to injections of a morphine solution had the characteristics of a primary immune response. Opiate addicts differed from normal controls in the level of antibodies to morphine of IgM class. Elevated levels of such antibodies could be detected in about 70-80% of addicts with regular longterm opiate use when the time since the last drug intake did not exceed 60 days. Antibodies to morphine could serve as indicators of chronic opiate use especially in periods long after the last drug intake.
Asunto(s)
Anticuerpos/análisis , Dependencia de Morfina/inmunología , Morfina/inmunología , Adulto , Animales , Especificidad de Anticuerpos/inmunología , Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunoglobulinas/análisis , Masculino , Ratones , Dependencia de Morfina/diagnóstico , Trastornos Relacionados con Opioides/diagnóstico , Trastornos Relacionados con Opioides/inmunología , RatasRESUMEN
In human brain tumors of neuro-ectodermal and meningo-vascular series, using the method of enzymoelectrophoresis and specific tetrazole blue staining, three isoforms of hexokinase were revealed, these differ from each other by their activity and electrophoretic mobility in agar gel. Three isoforms of hexokinase were also found in benign and malignant uterine tumors in females, in 22 A mice hepatoma and homologous intact tissues. Morever, in muscles and muscle tumors (MOP, CRM-1) of rats and of these animals embryos two isoforms of hexokinase were found. The increased rate of hexose phosphorylation in malignant uterine tumors of female patients and in blastomas of mice liver and rat muscles is associated with the increased activity of I and II isoforms of hexokinase. An analogous phenomenon is observed in muscles of rat embryos. On the other hand, the decreased activity of phosphotransferases in blastomas of human brain depends on a decrease in the activity of separate isoforms of hexokinase.
Asunto(s)
Hexoquinasa/metabolismo , Isoenzimas/metabolismo , Neoplasias/enzimología , Animales , Femenino , Humanos , Ratones , Neoplasias Experimentales , RatasRESUMEN
Thyrosine-I-C14 and serine-3-C14 show a higher level of incorporation into total soluble proteins of human gastric blastoma hemogenates than those of the mucous membrane. Moreover, a low radioactivity of pepsinogen was detected in the tumors. Also, there was a different rate of incorporation of these aminoacids both into separate fractions of soluble protein and pepsinogen of the mucous membrane and cancer of the stomach. These differences seem to be conditioned by alterations of the process of regulation of protein biosynthesis in the tumors. Simultaneously, there is a direct dependence of the proteolytic activity of pepsin isoforms on the rate of incorporation of the mucous membrane and cancer of human stomach.